Dulbecco s modified eagle medium low glucose dmem lg

The present study was approved by the Biosafety Board at The University of Lahore, Lahore, Pakistan. Samples were collected after obtaining written informed consent from mothers and all samples were screened for the absence of hepatitis B virus, hepatitis C virus, and human immunodeficiency virus. Samples were collected in sterile tubes and transported to the tissue culture laboratory immediately after collection. WJMSCs were isolated from umbilical cords by an explant culturing method as we previously described.⁸ (link) Briefly, cord pieces were incubated in 3 mg/ml collagenase solution (Invitrogen, Carlsbad, CA, USA). After 3 h, Dulbecco's modified eagle medium-low glucose (DMEM LG) (Sigma Aldrich, St. Louis, MO, USA) containing 10% human serum, 100 U/ml penicillin, and 100 μg/ml streptomycin (Sigma–Aldrich) was added to the same flasks (Corning Inc., Corning, NY, USA) containing collagenase solution and placed at 37 °C in an incubator at 95% humidity and 5% CO₂. The medium was renewed after three days. Cells were used at passage 3 for all further experiments.