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Vitalview

Manufactured by Philips
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VitalView is a versatile laboratory equipment designed to monitor and record vital signs. It provides real-time data collection and visualization of physiological parameters such as heart rate, respiration, and temperature.

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Lab products found in correlation

Mini mitter, by Philips (2 mentions) Clocklab, by Actimetrics (2 mentions) G2 e mitter, by Starr Life Sciences (1 mentions) Rodent diet 5001, by Land O'Lakes (1 mentions)

7 protocols using vitalview

1

Circadian Rhythm Monitoring in Mice

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Mice were housed individually in cages with a 4.5 inch wheel and their activity was measured by wheel rotations collected in 5-min bins with VitalView software (MiniMitter; Respironics). Mice were placed in 12 h light – 12 h dark (12:12 LD) cycles at ~700 lux for at least 2 weeks, followed by darkness (DD) for 2–3 weeks, and placed in 12:12 LD again for 2 weeks. Custom MATLAB scripts were used to generate actograms and measure activity onset to calculate period lengths. The wheel running activity of mice exposed to changing light paradigms is recorded in 5-min bins double plotted.
Beier C., Bocchero U., Levy L., Zhang Z., Jin N., Massey S.C., Ribelayga C.P., Martemyanov K., Hattar S, & Pahlberg J. (2022). Divergent outer retinal circuits drive image and non-image visual behaviors. Cell reports, 39(13), 111003.
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2

Acclimatization and Surgical Procedures for Rat Studies

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Rats were handled for 1 week each day to become accustomed to the researchers and experimental procedures. Three days before surgery, rats were individually housed in a cage to measure food intake and body weight. These cages record every interaction with the feeder by the interruption of an infrared sensor, which is detected by a computerized data acquisition system (VitalView, Respironics, Inc., Murraysville, PA, United States). Prior to surgery, the rats were kept at an ambient temperature of 22°C in a clean cage. All boxes were located at the same height, same light intensity and free of noise pollution. Rats were anesthetized via an intraperitoneal injection of a ketamine/xylazine (90 and 10 mg/kg, respectively) cocktail. Reflex loss was assessed through leg retraction, corneal response, and whisker movement. Subsequently, the hair was shaved from the head, and a 1% ketoprofen (10 mg/mL) and tramadol (2 mg/kg) cocktail was injected into the loin.
Barahona M.J., Rojas J., Uribe E.A, & García-Robles M.A. (2020). Tympanic Membrane Rupture During Stereotaxic Surgery Disturbs the Normal Feeding Behavior in Rats. Frontiers in Behavioral Neuroscience, 14, 591204.
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3

Circadian Rhythms in Mice After LPS

Cited 1 time
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For telemetry, G2 emitters (Starr Life Sciences) were implanted into the IP cavity of anesthetized mice. CBT was recorded in Vitalview (Respironics) and analyzed in Clocklab (Actimetrics). Wheel running was assayed as described [17 (link)]. After pre-conditioning with 1X LPS in 12:12 LD during entrainment, mice were moved to DD for 2-3 weeks, then given 3X LPS, followed by 1X LPS 2-3 weeks later. See Supplement for LPS protocol details.
Bedont J.L., LeGates T.A., Buhr E., Bathini A., Ling J.P., Bell B., Wu M.N., Wong P.C., Van Gelder R.N., Mongrain V., Hattar S, & Blackshaw S. (2016). An Lhx1-Regulated Transcriptional Network Controls Sleep/Wake Coupling and Thermal Resistance of the Central Circadian Clockworks. Current biology : CB, 27(1), 128-136.
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4

Circadian Rhythm Monitoring in Mice

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Mice were housed individually in cages with a 4.5 inch wheel and their activity was measured by wheel rotations collected in 5-min bins with VitalView software (MiniMitter; Respironics). Mice were placed in 12 h light – 12 h dark (12:12 LD) cycles at ~700 lux for at least 2 weeks, followed by darkness (DD) for 2–3 weeks, and placed in 12:12 LD again for 2 weeks. Custom MATLAB scripts were used to generate actograms and measure activity onset to calculate period lengths. The wheel running activity of mice exposed to changing light paradigms is recorded in 5-min bins double plotted.
Beier C., Bocchero U., Levy L., Zhang Z., Jin N., Massey S.C., Ribelayga C.P., Martemyanov K., Hattar S, & Pahlberg J. (2022). Divergent outer retinal circuits drive image and non-image visual behaviors. Cell reports, 39(13), 111003.
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5

Voluntary Wheel Running as Fatigue Proxy

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We used voluntary wheel running activity (VWRA) as an objective proxy for fatigue. We monitored VWRA for each mouse continuously throughout the experimental procedure using the VitalView data acquisition system (Respironics, Sunriver, OR) with an epoch of 60 min. We measured food intake and body weight twice each week (Tuesday and Friday) between 6:00–7:00 a.m. Rodent Diet 5001 (PMI Nutrition International, Brentwood, MO) was placed in the food hopper, and the amount eaten was determined by weighing the food remaining in the hopper. After a two-week acclimation period, each mouse was allowed to establish a 10-day baseline VWRA, after which they were separated into groups. In experiment 1, wild-type mice were divided into two groups. Mice in group 1 (n = 20) received EBRT (as described below) while mice in group 2 (n = 20) were sham irradiated.
In experiment 2, wild-type and TNF-α-deficient mice were separated into two groups, respectively (n = 10/group). Wild-type and TNF-α-deficient mice received EBRT as described below while the remaining wild-type and TNF-α-deficient mice were sham irradiated. After treatment mice were returned to their home cages.
McDonald T.L., Hung A.Y., Thomas CR J.r, & Wood L.J. (2015). Localized External Beam Radiation Therapy (EBRT) to the Pelvis Induces Systemic IL-1Beta and TNF-Alpha Production: Role of the TNF-Alpha Signaling in EBRT-Induced Fatigue. Radiation research, 185(1), 4-12.
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6

Continuous Behavioral Rhythm Monitoring

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Free-running behavior was recorded continuously. Wheel revolutions per 10 min bin were stored on a computerized data acquisition system (VitalView, Respironics, Inc, Murraysville, PA: currently Starr Life Sciences, Oakmont, PA). Period and daily onset of activity bouts was performed using Clocklab (Actimetrics, Wilmette, IL, USA). To calculate the precision of onset of activity, the daily difference between the actual and the projected free-running onset time was tracked, using Clocklab. The standard deviation of these daily differences is reported as the precision. Thus, the smaller the standard deviation, the more precise is the animal’s onset from day to day.
Model Z., Butler M.P., LeSauter J, & Silver R. (2015). Suprachiasmatic Nucleus as the Site of Androgen Action on Circadian Rhythms. Hormones and behavior, 73, 1-7.
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7

Feeding Behavior Microstructure Analysis in Rats

Cited 3 times
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Feeding behavior evaluated in rats was recorded by a computerized data acquisition system (VitalView, Respironics, Inc.), which individually registers the number of times each rat interacted with the feeder and the amount of time that they remained in the trough. A meal event (ME) was defined as one or more episodes longer than 5 s and no longer than 15 min, followed by a meal interval (MI) (Barahona et al., 2018 (link)). The minimum MI was defined as 10 min, as previously described (Elizondo-Vega et al., 2016 (link); Uranga et al., 2017 (link); Barahona et al., 2018 (link)). Microstructure parameters were evaluated. The mean meal size was determined as the total food intake (g) divided by meal frequency. The mean meal duration was calculated by dividing the total meal duration (min) by total meal events, and the eating rate was estimated by dividing total food intake (g) by total meal duration (min).
Barahona M.J., Rojas J., Uribe E.A, & García-Robles M.A. (2020). Tympanic Membrane Rupture During Stereotaxic Surgery Disturbs the Normal Feeding Behavior in Rats. Frontiers in Behavioral Neuroscience, 14, 591204.
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