Co2 incubator

Manufactured by Sanyo

Sourced in Japan, United States, China

The CO2 incubator is a laboratory equipment designed to provide a controlled environment for cell culture and other applications requiring a specific atmospheric composition. The incubator maintains a stable temperature and regulates the carbon dioxide (CO2) level within the chamber to support the growth and development of cells or organisms.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

CO2 humidified incubator (6 citations) IR direct heat CO2 incubator (5 citations) MCO-18A1C CO2 incubator (4 citations) MCO-5AC CO2 Incubator (4 citations) MCO-15AC CO2 incubator (3 citations) MCO-18AIC CO2 Incubator (3 citations) SANYO AUTOMATIC CO2 INCUBATOR (2 citations) MCO-20AIC CO2 incubator (2 citations) MCO-18M O2/CO2 incubator (2 citations)

88 protocols using co2 incubator

Micromolded Scaffolds for Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Scaffolds were made by micromolding. On the day of the experiment, 12 PVA frames were placed in sterile Petri dishes (Corning, USA) of 35 mm in diameter, 3 pieces in each dish. Then, 6 frames were filled with chilled collagen-based hydrogel, added with warm nutrient medium, and placed in a CO₂ incubator (Sanyo, Japan) (37°C, 5% CO₂) for polymerization. The remaining 6 frames were placed on an ice pack, filled with warm GelMA-based hydrogel, kept for 30 min, then filled with cold nutrient medium, and irradiated with ultraviolet light (365 nm) in the internal space of the printer for 10 min at a temperature of the printing table of 0…+4°C. After irradiation, the frames were kept for another 30 min in cold, then placed in a CO₂ incubator (Sanyo, Japan).
A few hours later, when the PVA frames were subjected to dissolution, the obtained scaffolds were washed with phosphate-buffered saline to remove PVA remains, placed in new sterile Petri dishes, added with DMEM medium (glucose content — 4.5 g/L) and 10% fetal bovine serum, and left in the incubator until the next day. After polymerization of hydrogels and PVA dissolution, the finished structures had a lattice form (Figure 2).

Isaeva E.V., Kisel A.A., Beketov E.E., Demyashkin G.A., Yakovleva N.D., Lagoda T.S., Arguchinskaya N.V., Baranovsky D.S., Ivanov S.A., Shegay P.V, & Kaprin A.D. (2023). Effect of Collagen and GelMA on Preservation of the Costal Chondrocytes’ Phenotype in a Scaffold in vivo. Modern Technologies in Medicine, 15(2), 5-16.

+ Open protocol

+ Expand

Culturing Human Breast MCF-7 Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human breast MCF-7 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS and 1% penicillin/streptomycin mixture. The cells were maintained in a humidified atmosphere of 5% CO₂ at 37 °C (Sanyo CO2 Incubator; Sanyo, Kitanagoya, Aichi)^{37 (link)}.

Khodayari H., Khodayari S., Khalighfard S., Tahmasebifar A., Tajaldini M., Poorkhani A., Nikoueinejad H., Hamidi G.A., Nosrati H., Kalhori M.R, & Alizadeh A.M. (2021). Gamma-radiated immunosuppressed tumor xenograft mice can be a new ideal model in cancer research. Scientific Reports, 11, 256.

+ Open protocol

+ Expand

Culturing Human Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used cell lines of human fibrosarcoma (cell culture HT-1080) and breast cancer (MCF-7) obtained at the Research Institute of Virology (D. I. Ivanovsky Institute of Virology, Moscow, Russia). Tumor cells were cultured according to the recommendations specified in the cell culture certificates. Cells were incubated in DMEM culture media supplemented with 2 mM L-glutamine and 10% FBS in 25 cm² and 75 cm² culture flasks (Corning, Glendale, AZ, USA) at 37 °C in a humid atmosphere with 5% CO₂ in a Sanyo CO₂ incubator (Sanyo, Moriguchi, Japan). Cell lines were used in the study after 3 to 10 passages.

Kostryukova L.V., Tereshkina Y.A., Tikhonova E.G., Khudoklinova Y.Y., Bobrova D.V., Gisina A.M., Morozevich G.E., Pronina V.V., Bulko T.V, & Shumyantseva V.V. (2023). Effect of an NGR Peptide on the Efficacy of the Doxorubicin Phospholipid Delivery System. Nanomaterials, 13(15), 2229.

+ Open protocol

+ Expand

Culturing MCF-7 and MDA-MB-231 Breast Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

MCF-7 cells (ATCC^® HTB-22™, ATCC, Manassas, VA. USA) and MDA-MB-231 cells (ATCC^® HTB-26™; ATCC, Manassas, VA, USA) were grown in Dulbecco’s modified Eagle’s medium (Gibco^® Life Technologies, Carlsbad, CA. USA) supplemented with 10% fetal bovine serum (DMEM 10% FBS) and 1% penicillin/streptomycin mixture. Cells were maintained in a humidified atmosphere of 5% CO₂ at 37 °C (Sanyo CO₂ Incubator; Sanyo, Japan). All experiments were performed in triplicate and repeated three times.

Marinello P.C., Panis C., Silva T.N., Binato R., Abdelhay E., Rodrigues J.A., Mencalha A.L., Lopes N.M., Luiz R.C., Cecchini R, & Cecchini A.L. (2019). Metformin prevention of doxorubicin resistance in MCF-7 and MDA-MB-231 involves oxidative stress generation and modulation of cell adaptation genes. Scientific Reports, 9, 5864.

+ Open protocol

+ Expand

Ac-KEKK-NH2 Enhances Neurite Growth

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Ac-KEKK-NH₂ effect on neurite growth was studied on DRG explants from 10 to 12-day-old chicken embryos. Explants were placed in collagen-coated sterile Petri dishes and cultured in the medium composed of 45% Hank’s solution and 40% Eagle’s medium supplemented with insulin (0.5 U/mL), glucose (0.6%), L-glutamine (2 mM), gentamicin (100 U/mL), 10% fetal bovine serum, and Ac-KEKK-NH₂ at the studied concentrations for three days in the CO₂ incubator (Sanyo, Osaka, Japan) at 37 °C and 5% CO₂. Control explants were cultured without Ac-KEKK-NH₂. Explants were visualized with an Axio Observer Z1 microscope (Carl Zeiss, Oberkochen, Germany) and analyzed with ImageJ (National Institutes of Health, Bethesda, MD, USA) and ZEN_2012 (Carl Zeiss, Germany) software to evaluate the neurite growth using the area index (AI), which uses the ratio of the peripheral growth zone area to the central zone area. The average AI value in the control explants was taken as 100% [9 (link)]. Experiments were conducted using the equipment of the Confocal Microscopy Collective Use Center at the Pavlov Institute of Physiology, Russian Academy of Sciences.

Kalinina A.D., Rogachevskii I.V., Samosvat D.M., Zegrya G.G., Butkevich I.P., Mikhailenko V.A., Plakhova V.B., Penniyaynen V.A., Podzorova S.A, & Krylov B.V. (2023). Analgesic Effect of the Lysine-Containing Short Peptide Is Due to Modulation of the NaV1.8 Channel Activation Gating System. Life, 13(9), 1800.

+ Open protocol

+ Expand

In Vitro Culture of Human Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cells were obtained from the Bioresource Collection and Research Center (Hsinchu, Taiwan). Human colon cancer cells (HT-29) was cultured in RPMI 1640 (HyClone, South Logan, UT, USA) medium, supplemented with 10% fetal bovine serum (FBS, Gibco, Life Technologies, Carlsbad, CA, USA) and antibiotics (100 mg/mL streptomycin and 100 U/mL penicillin, Gibco, Life Technologies, USA). Human bladder cancer cells (BFTC 905) and human liver carcinoma (HepG2) cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM, HyClone, USA) medium, supplemented with 10% fetal bovine serum (FBS) and antibiotics. Human cancer cells were maintained in humidified atmosphere with 5% CO₂ and 95% air at 37 °C in a carbon dioxide incubator (SANYO, CO₂ incubator, Osaka, Japan).

Lin H.J., Ho J.H., Tsai L.C., Yang F.Y., Yang L.L., Kuo C.D., Chen L.G., Liu Y.W, & Wu J.Y. (2020). Synthesis and In Vitro Photocytotoxicity of 9-/13-Lipophilic Substituted Berberine Derivatives as Potential Anticancer Agents. Molecules, 25(3), 677.

+ Open protocol

+ Expand

Isolation and Culture of Intervertebral Disc Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue samples were weighed and washed twice in phosphate-buffered saline (PBS). The NP and AF were separated based on their macroscopic morphologies by omitting the transitional zone, as previously described (6 (link)). Cells from each patient were isolated and cultured separately to avoid interference. The NP tissue samples were sectioned (~1 mm²) prior to being digested with 0.25% trypsinase (GE Healthcare Life Science) at 37°C under gentle agitation. After 20 min, digestion was stopped using DMEM-F12 supplemented with 15% fetal calf serum, and the tissue samples were centrifuged at 560 × g for 5 min. Subsequently, 0.5% collagenase Type II (ICN Biomedicals Inc., Costa Mesa, CA, USA) was used to treat the cells at 37°C for ~4 h. The tissue samples were centrifuged at 560 × g for 5 min and washed three times with DMEM-F12 supplemented with 15% fetal calf serum.
The cells were transferred to a 12.5-cm² culture flask at a density of 10⁵ cells/ml. The cells were maintained in DMEM-F12 supplemented with 15% fetal calf serum and 1% penicillin/streptomycin (Invitrogen; Thermo Fisher Scientific, Inc.), and cultured in a CO₂ incubator (SANYO Electric Co. Ltd., Moriguchi, Japan) at 37°C with humidity (95%). The growth medium was changed every three days following cell adherence. All experiments were conducted during passage two, and sub-confluent cultures were used.

LIN Y., YUE B., XIANG H., LIU Y., MA X, & CHEN B. (2015). Survivin is expressed in degenerated nucleus pulposus cells and is involved in proliferation and the prevention of apoptosis in vitro. Molecular Medicine Reports, 13(1), 1026-1032.

+ Open protocol

+ Expand

Culturing HepG2 Cells in RPMI Medium

Check if the same lab product or an alternative is used in the 5 most similar protocols

HepG2 cells were cultured in RPMI 1640 medium supplemented with 10% of inactivated FBS, 100 U/mL penicillin, and 100 µg/mL streptomycin in a CO 2 incubator (SANYO, Tokyo, Japan) at 37°C and 5% CO 2 . The culture medium was replaced once every 3 to 4 days.

Tsubonoya T., Inoue E., Sudo K., & Shimizu Y. (2021). Induction of Antioxidant and Detoxifying Enzymes via the Nrf2-ARE Pathway by Oriental Bezoar.

+ Open protocol

+ Expand

Gastric Cancer Cell Line Bile Acid Exposure

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For our purposes, we required well-established, acid-stable gastric cancer cell lines with comparable levels of c-Myc expression (10 (link)). Accordingly, we purchased AGS (ATCC^® CRL-1739^™) and NCI-N87 (ATCC^® CRL-5822^™) cell lines from the American Type Culture Collection (Manassas, VA, USA). These gastric cancer cell lines were grown in Dulbecco's modified Eagle's medium (DMEM) (GIBCO Invitrogen) containing 4.5 mg/l glucose, 100 mg/l streptomycin, and 2 mM L-glutamine supplemented with 10% fetal bovine serum (FBS) (GIBCO Invitrogen). They were maintained at 37°C under a humidified 5% CO₂ atmosphere in a CO₂ incubator (Sanyo). Solutions of bile acids (Sigma-Aldrich) were prepared using appropriate solvents according to the manufacturer's protocols (Table SI). AGS and NCI-N87 cells were cultured in the growth medium for 24 h and then transferred to fresh, serum-free medium containing 100 µM of a bile acid for 48 h, with the bile acid being cholic acid (CA; Sigma-Aldrich, C9377), chenodeoxycholic acid (CDCA; Sigma-Aldrichl, C1129), taurocholic acid (TCA; Sigma-Aldrich, T4009), or glycochenodeoxycholic acid (GCDCA; Sigma-Aldrich, G0759). Afterward, we extracted the total RNA and total protein from the cells.

Lee S.M., Park M.S., Park S.Y., Choi Y.D., Chung J.O., Kim D.H., Jung Y.D, & Kim H.S. (2022). Primary bile acid activates Egr-1 expression through the MAPK signaling pathway in gastric cancer. Molecular Medicine Reports, 25(4), 129.

+ Open protocol

+ Expand

Bioluminescence Imaging of Clathrin in HeLa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HeLa (RIKEN BRC) cells were cultured on collagen-coated 35 mm glass-bottom dishes in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS). The next day, HeLa cells (∼70% confluency) were transfected with 4.0 μg plasmid DNA using Lipofectamine 2000 Transfection Reagent (Life Technologies) according to the manufacture's recommended protocol. Medium was replaced after 8 h, and the cells grown for an additional 16 h in a CO₂ incubator (Sanyo) at 37 °C in 5% CO₂. HeLa cells were washed with phenol red-free DMEM/F12 and imaged in phenol red-free DMEM/F12. Just before observation, 20 μM furimazine was added to the imaging medium. To observe eNL signals in living HeLa cells, an inverted microscope (LV-200, Olympus) equipped with a × 100 objective (Olympus, UPlanSApo, numerical aperture 1.4) and × 0.5 relay lens was used. Emission signals were detected by an EM-CCD camera (ImagEM, Hamamatsu Photonics) with 1 × 1 (for eNL) or 2 × 2 (for GeNL(Ca²⁺)) binning settings. To observe the localization of clathrin light chain labelled with CeNL in living HeLa cells, an inverted microscope (IX83, Olympus, Japan) equipped with a × 100 objective (Olympus, UPlanSApo, numerical aperture 1.4) was used. Emission signals were detected by an EM-CCD camera (Evolve 512, Photometrics) with 1 × 1 binning.

Suzuki K., Kimura T., Shinoda H., Bai G., Daniels M.J., Arai Y., Nakano M, & Nagai T. (2016). Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nature Communications, 7, 13718.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!