Ab2415

Manufactured by Abcam

Sourced in United Kingdom

Ab2415 is a laboratory equipment product. It is a device used for scientific research purposes. The core function of this product is to perform specific tasks required in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Pa1 186, by Thermo Fisher Scientific (1 mentions) Ab29045, by Abcam (1 mentions) Ab133566, by Abcam (1 mentions) Haematoxylin and bluing reagent, by Roche (1 mentions) Cell conditioning 1, by Roche (1 mentions) Ultraview dab detection kit, by Roche (1 mentions) Ab17147, by Abcam (1 mentions) Axio imager a1 fluorescent microscope, by Zeiss (1 mentions) A21422, by Thermo Fisher Scientific (1 mentions) Dp70 microscope camera, by Olympus (1 mentions) Ab47435, by Abcam (1 mentions) Ab138005, by Abcam (1 mentions)

3 protocols using ab2415

Antibody Panel for Cerebrovascular Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies: Rat monoclonal anti-Pecam-1/CD31 (Clone MEC 13.3, BD Pharmingen, San Jose, CA, USA); rabbit polyclonal anti-STAT1 (ab2415, Abcam, Cambridge, UK); mouse monoclonal anti-STAT1 pY701 (ab29045, Abcam, Cambridge, UK); rabbit polyclonal anti-NFκB/p65 (PA1–186, Thermo Fisher, Waltham, MA, USA); rabbit polyclonal anti-NFκB/p65 pThr435 (PA5–37724, ThermoFisher, Waltham, MA, USA); mouse monoclonal Anti-γ Interferon (ab133566, Abcam, Cambridge, UK).
Secondary antibodies: Alexa594-conjugated goat anti-rat antibodies (Pecam-1 immunolabeling on brain slices), Horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit (Western-blot).

Boulay A.C., Gilbert A., Oliveira Moreira V., Blugeon C., Perrin S., Pouch J., Le Crom S., Ducos B, & Cohen-Salmon M. (2018). Connexin 43 Controls the Astrocyte Immunoregulatory Phenotype. Brain Sciences, 8(4), 50.

+ Open protocol

+ Expand

Evaluating STAT1 Expression in High-Grade Serous Ovarian Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumour STAT1 expression was evaluated using immunohistochemistry (IHC) on an HGSC tissue microarray (TMA) (TFRI‐COEUR) consisting of 0.6 mm diameter FFPE tumour cores, in duplicate, from a cohort of 734 chemotherapy naïve HGSC cases. This large multicentre TFRI‐COEUR cohort contained 550 chemotherapy naïve HGSC cases that formed our STAT1 second independent validation cohort in addition to duplicates for the 184 cases (designated as CHUM cohort; first independent validation 7) from our previous study. The rabbit anti‐human polyclonal STAT1 primary antibody (1:2000 dilution, Abcam #ab2415) 7 and mouse anti‐human monoclonal CD8 primary antibody (1:25 dilution, Abcam # ab17147) were applied on separate TMA slides using the Ventana automated immunostaining system. In brief, antigen retrieval was carried out with Cell Conditioning 1 (Ventana Medical System Inc.) for 60 min. The slides were incubated with primary STAT1 or CD8 antibody with appropriate negative controls, at 37°C for 60 min. Reactions were carried out using the ultraView DAB detection kit (Ventana Medical System Inc.). Slides were counterstained with haematoxylin and bluing reagent (Ventana Medical System Inc.) for 4 min. The IHC stained TMAs were scanned on Aperio Scanscope and digitally conserved.

Au K.K., Le Page C., Ren R., Meunier L., Clément I., Tyrishkin K., Peterson N., Kendall‐Dupont J., Childs T., Francis J., Graham C.H., Craig A.W., Squire J.A., Mes‐Masson A, & Koti M. (2016). STAT1‐associated intratumoural TH1 immunity predicts chemotherapy resistance in high‐grade serous ovarian cancer. The Journal of Pathology: Clinical Research, 2(4), 259-270.

+ Open protocol

+ Expand

Immunofluorescence Analysis of JAK-STAT Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

COV434 cell slides were prepared and probed with antibodies specific for JAK1 (ab47435, Abcam), pJAK1 (ab138005, Abcam), STAT1 (ab2415, Abcam) and STAT3 (79D7, Cell Signalling Technologies). Primary antibodies were visualised using either a goat-anti-rabbit Alexa 555 secondary antibody (ab150078, Life Technologies) or a goat-anti-mouse Alexa 555 secondary antibody (a21422, Life Technologies) at a 1:100 dilution and 4′-6-Diamidino-2-phenylindole (DAPI) was used as a nuclear counterstain. Slides were imaged using the Axio Imager A1 fluorescent microscope (Carl Zeiss MicroImaging, Inc, Thornwood, NY) and Olympus DP70 microscope camera (Olympus America, Center Valley, PA).

Frost E., Ford E., Peters A., Reed N., McLaughlin E., Baker M., Lovell-Badge R, & Sutherland J. (2020). STAT1 and STAT3 are expressed in the human ovary and have JAK1-independent functions in the COV434 human granulosa cell line. Reproduction, fertility, and development, 32(12), 1027-1039.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!