Anti goat igg a5420

Manufactured by Merck Group

Sourced in United States

Anti-Goat IgG (A5420) is a laboratory reagent that binds to immunoglobulin G (IgG) antibodies derived from goats. It is commonly used in various immunoassays and techniques that require the detection or isolation of goat-derived IgG.

Automatically generated - may contain errors

Lab products found in correlation

Actin d35e4, by Santa Cruz Biotechnology (2 mentions) Anti guinea pig igg a7289, by Merck Group (2 mentions) Gfap mab360, by Merck Group (1 mentions) Tau 5 ab80579, by Abcam (1 mentions) Mab360, by Merck Group (1 mentions) Anti rabbit igg a0545, by Merck Group (1 mentions) Iblot system, by Thermo Fisher Scientific (1 mentions) Ripa buffer, by Merck Group (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) Anti rabbit igg a6154, by Merck Group (1 mentions) Biotinylated anti mouse igg, by Vector Laboratories (1 mentions) Anti mouse igg a4416, by Merck Group (1 mentions)

3 protocols using anti goat igg a5420

Immunohistochemical Analysis of Cortex Samples

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cortex samples were prepared using standard techniques and probed using the following primary antibodies: GFAP (MAB360, 1:2,000; Millipore, Bedford, MA)(Buckman, et al., 2013 (link)), Neprilysin/CD10 (Ab79423, 1:1,000), Receptor for Advanced Glycation End Products (AGEs), RAGE (Ab37674, 1:1,000), Tau-5 (ab80579, 1:400) and phospo-Tau (S396; ab109390, 1:5,000) all from Abcam (Cambridge, MA). TNFa (D2D4, 1:500-1:1,000), and Synaptophysin (D35E4, 1:1,000) Cell Signaling (Danvers, MA), and Actin (D35E4, 1:400-1,5,000; Santa Cruz Biotechnology, Dallas Texas). Appropriate secondary antibodies were selected from Anti-Goat IgG (A5420, 1:5,000), Anti-Guinea Pig IgG (A7289, 1:5,000) and Anti-Rabbit IgG (A0545, 1:5,000) all from Sigma-Aldrich (USA).

Walker J., Dixit S., Saulsberry A., May J, & Harrison F. (2017). Reversal of high fat diet-induced obesity improves glucose tolerance, inflammatory response, β-amyloid accumulation and cognitive decline in the APP/PSEN1 mouse model of Alzheimer’s disease. Neurobiology of disease, 100, 87-98.

+ Open protocol

+ Expand

Protein Expression Analysis in Cortical Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cortical tissue was homogenized using RIPA buffer (Sigma Aldrich, USA) with protease inhibitors (cOmplete protease inhibitor cocktail, Roche, Switzerland). Gels were loaded with 10 μg of protein and membranes were prepared using the iBlot system (LifeTechnologies, USA). Membranes were incubated with GLT-1 (AB1783, 1:4,000) and GFAP (MAB360, 1:2,000; both Millipore, Bedford, MA), xCT (NB-300–318, Novus Biologicals, Littleton, CO, USA), and Actin (D35E4, 1:5,000; Santa Cruz, USA). Appropriate secondary antibodies were selected from Anti-Goat IgG (A5420, 1:5,000), Anti-Guinea Pig IgG (A7289, 1:5,000) and Anti-Rabbit IgG (A0545, 1:5,000) all from Sigma-Aldrich (USA)

Mi D.J., Dixit S., Warner T.A., Kennard J.A., Scharf D.A., Kessler E.S., Moore L.M., Consoli D.C., Bown C.W., Eugene A.J., Kang J.Q, & Harrison F.E. (2018). Altered glutamate clearance in ascorbate deficient mice increases seizure susceptibility and contributes to cognitive impairment in APP/PSEN1 mice. Neurobiology of aging, 71, 241-254.

+ Open protocol

+ Expand

Immunoblotting for cell junction proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies were purchased and used at a dilution of 1:500 (unless indicated otherwise) as follows: HAVcR-1 Pab13202, (Abnova, Heyford, Oxon, UK); TIM-1/HAVcR-1 AF1817, E-cadherin 17029 (R&D Systems, Abingdon, Oxon, UK); TIM-1 (N-13 HAVcR-1) SC47495, GAPDH SC32233, Claudin-1 SC17658, Claudin-7 SC177670, Occludin SC8145, ZO-1 SC8146, PKM2 SC65176, cyclin D1 SC753 (Insight Biotechnology LTD, Middlesex, UK); α-catenin C1620 (BD Transduction Laboratories, San Jose, CA, USA); β-catenin 8415 (Sigma-Aldrich, Gillingham, Dorset, UK); Eplin A300-103A (Bethyl Labs, Montgomery, TX, USA). Secondary antibodies were prepared as per manufacturer’s instructions and were purchased as follows: Anti-Mouse IgG A4416, anti-Rabbit IgG A6154, anti-Goat IgG A5420 (Sigma-Aldrich, Gillingham, Dorset, UK); Biotinylated anti-Mouse IgG (Vector Labs, Orton Southgate, Peterborough, UK); AlexaFluor 488 anti-Rabbit, anti-Mouse, anti-Goat and AlexaFluor 594 anti-Rabbit, anti-Goat, DAPI (Thermo Fisher Scientific, Cramlington, UK).

Telford E.A., Sanders A.J., Owen S., Ruge F., Harrison G.M., Jiang W.G, & Martin T.A. (2022). Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity. Biomolecules, 12(2), 338.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!