Some of the collected blood is used to examine lipid profiles (HDL, LDL, triglycerides) as a marker of dyslipidemia and Hemoglobin A1c as a marker of impaired glucose tolerance. Individuals with an A1c level at or above 6.5 % who do not report a history of diabetes are informed of the result, provided counseling, and encouraged to follow up with their primary care providers. Hypertension is assessed by measuring diastolic and systolic blood pressure with an OMRON IntelliSense blood pressure monitor using a standard protocol.
Intellisense blood pressure monitor
Manufactured by Omron
The IntelliSense blood pressure monitor is a medical device designed to measure and display a person's blood pressure. It utilizes advanced sensing technology to provide accurate and reliable readings.
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Lab products found in correlation
4 protocols using intellisense blood pressure monitor
1
Metabolic Screening and Counseling
2
Comprehensive Blood and Metabolic Profiling
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After a ≥12-h fast, fasting blood samples were drawn by standard venipuncture protocols into evacuated vacutainer tubes (Fisher Scientific). Plasma was separated by centrifugation at 1100 × g at 4°C for 20 min and archived in 0.5-mL aliquots at −80°C until analysis. ESRs were determined immediately following each blood draw. Anthropometric measurements, including weight, height, and body composition (body fat percentage, fat mass, fat-free mass, total body water, and basal metabolic rate), were measured at each visit by bioelectrical impedance (TBF 300A Tanita Body Composition Analyzer). Dietary records and physical activity questionnaires were collected and reviewed at each visit. Blood pressure and pulse rate were determined with an automated IntelliSense blood pressure monitor (Omron Healthcare, Inc.).
Total cholesterol, TGs, LDL cholesterol, HDL cholesterol, fasting glucose, and hsCRP were measured directly from plasma samples with the use of a COBAS C111 blood chemistry analyzer (Roche Diagnostics) and VLDL cholesterol was calculated as TG/5. Plasma insulin was analyzed with an Immulite 1000 automated chemiluminescent blood chemistry analyzer (Diagnostic Products Corp., Siemens). MCP-1 was analyzed by standard sandwich enzyme-linked immunosorbent assay performed with single-analyte ELISArray kits (SABiosciences Corp.).
Total cholesterol, TGs, LDL cholesterol, HDL cholesterol, fasting glucose, and hsCRP were measured directly from plasma samples with the use of a COBAS C111 blood chemistry analyzer (Roche Diagnostics) and VLDL cholesterol was calculated as TG/5. Plasma insulin was analyzed with an Immulite 1000 automated chemiluminescent blood chemistry analyzer (Diagnostic Products Corp., Siemens). MCP-1 was analyzed by standard sandwich enzyme-linked immunosorbent assay performed with single-analyte ELISArray kits (SABiosciences Corp.).
3
Automated Blood Pressure Monitoring
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Blood pressure (BP) is measured with an automated BP monitor (OMRON IntelliSense Blood Pressure Monitor) by trained study staff. Three consecutive measurements are taken with a one-minute rest between each measure. The American Heart Association guidelines for blood pressure were used to classify the results: normal BP (<120/80 mmHg), elevated BP (120–129/<80 mmHg), stage 1 hypertension (130–139/80–89 mmHg), and stage 2 hypertension (≥140/90 mmHg) (Flack et al., 2020 (link)). Participants are emailed and advised by our study’s physician to consult with their primary care physician, if their blood pressure fell in any of the following categories: Elevated, Stage 1, Stage 2, or Hypertension Crisis.
4
Cardiovascular Physiological Assessment
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Blood Pressure. BP data were collected using an IntelliSense blood pressure monitor (Omron Corporation). The device measures BP three times, and the average of these three systolic and diastolic blood pressures is displayed. BP measurements occurred following five minutes of seated rest.
Heart Rate and High Frequency-Heart Rate Variability. Biopac MP150 for Windows (Biopac Systems Inc.) was used to collect resting cardiovascular physiological data (e.g. HR and HF-HRV) at a sampling rate of 1 kHz, amplified and digitized using the Biopac system. Following BP assessments, these data were collected over the course of a five-minute resting state recording. These data were processed using MindWare software (MindWare Technologies, Inc.). To obtain HR and HF-HRV data, MindWare identifies electrocardiogram R-peaks and R-R intervals (i.e. the time between successive heart beats). The system flags R-peaks that have been identified as improbable, and these R-peaks can then be manually examined and corrected. HF-HRV was derived by spectral analysis of one-minute epochs with a Hamming windowing function, a baseline and muscle noise filter, and log transformed. Settings for the high frequency band were based on standard recommendations for HF-HRV data (0.12–0.40 Hz; Task Force, 1996 (link)).
Heart Rate and High Frequency-Heart Rate Variability. Biopac MP150 for Windows (Biopac Systems Inc.) was used to collect resting cardiovascular physiological data (e.g. HR and HF-HRV) at a sampling rate of 1 kHz, amplified and digitized using the Biopac system. Following BP assessments, these data were collected over the course of a five-minute resting state recording. These data were processed using MindWare software (MindWare Technologies, Inc.). To obtain HR and HF-HRV data, MindWare identifies electrocardiogram R-peaks and R-R intervals (i.e. the time between successive heart beats). The system flags R-peaks that have been identified as improbable, and these R-peaks can then be manually examined and corrected. HF-HRV was derived by spectral analysis of one-minute epochs with a Hamming windowing function, a baseline and muscle noise filter, and log transformed. Settings for the high frequency band were based on standard recommendations for HF-HRV data (0.12–0.40 Hz; Task Force, 1996 (link)).
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