Orion star a211

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Orion Star A211 is a benchtop pH/mV/Temp meter designed for basic pH measurement and analysis. It features a large, easy-to-read LCD display, automatic buffer recognition, and temperature compensation. The Orion Star A211 is compatible with a wide range of pH, reference, and temperature electrodes for versatile applications.

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Lab products found in correlation

Icap 7000 icp oes spectrometer, by Thermo Fisher Scientific (2 mentions) Orion star a212, by Thermo Fisher Scientific (2 mentions) Inlab surface, by Mettler Toledo (2 mentions) Talos f200c g2, by Thermo Fisher Scientific (2 mentions) K alpha, by Thermo Fisher Scientific (2 mentions) Alex 500, by Anton Paar (1 mentions) Gf c filter paper, by Cytiva (1 mentions) Tnm l, by Shimadzu (1 mentions) Toc l cph, by Shimadzu (1 mentions) Digital micrometer, by Mitutoyo (1 mentions) Alexafluor647 hydrazide, by Thermo Fisher Scientific (1 mentions) Synergy water purification system, by Merck Group (1 mentions) Polytron pt 2500 e, by Kinematica (1 mentions) Toc 5000a, by Shimadzu (1 mentions) Pal 1, by Atago (1 mentions) 0.1 m hcl, by Merck Group (1 mentions) 0.1 m naoh, by Merck Group (1 mentions) Galaxy s21, by Samsung (1 mentions) Synergy h1, by Agilent Technologies (1 mentions) Orion star a222, by Thermo Fisher Scientific (1 mentions)

71 protocols using orion star a211

Measuring pH of Salted Chicken

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A total of 3 g of sample were homogenized with 27 mL of distilled water (DW) at 8,000 rpm for 1 min. The pH value of pre-/post-rigor salted chicken breasts was measured in triplicate using an electric pH meter (Orion star A211, Thermo scientific, Beverly, MA).

Song D.H., Ham Y.K., Ha J.H., Kim Y.R., Chin K.B, & Kim H.W. (2019). Impacts of pre-rigor salting with KCl on technological properties of ground chicken breast. Poultry Science, 99(1), 597-603.

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Measuring Wine pH and Alcohol

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The pH of wines was measured with a digital pH meter (ThermoScientific^®, model Orion Star A211 (Waltham, MA, USA). The alcohol content in red wines was analyzed using an alcohol and extract meter, Alex 500 (Anton Paar, Vernon Hills, IL, USA). The alcohol and extract meter is based on the near-infrared technique, and the alcohol content was evaluated from 20 mL of red wines at room temperature.

, & Watrelot A.A. (2021). Tannin Content in Vitis Species Red Wines Quantified Using Three Analytical Methods. Molecules, 26(16), 4923.

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Soil Geochemical Analysis Protocol

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Sediment pH was determined for all samples according to the method described in reference 71 (link). Specifically, 5 g of soil and 5 g of deionized (DI) water were placed in a 15-mL conical tube and shaken for 2 h at 200 rpm. Sediment pH was then measured with an Orion Star A211 benchtop pH meter (Thermo Fisher Scientific, Waltham, MA, USA). Other geochemical measurements were performed on freeze-dried and crushed aliquots of each sample. Total nitrogen content (TN) and total organic carbon (TOC) measurements for all samples were measured by the Arikaree Laboratory at the University of Colorado Boulder using a Shimadzu TOC-L/TNM-L TOC/TN analyzer. Additional geochemical analyses were performed on 25 of the samples by Activation Laboratories Ltd. (Ancaster, ON, Canada) with the composition of trace elements measured via lithium borate fusion inductively coupled plasma mass spectrometry (ICP-MS) and instrumental neutron activation analysis (INAA). Statistical tests to assess differences in the environmental and geochemical properties across sample categories were conducted using Mann-Whitney U tests for two groups or Kruskal-Wallis one-way analysis of variance tests with the post hoc Dunn’s multiple-comparison test for three or more.

Dragone N.B., Whittaker K., Lord O.M., Burke E.A., Dufel H., Hite E., Miller F., Page G., Slayback D, & Fierer N. (2023). The Early Microbial Colonizers of a Short-Lived Volcanic Island in the Kingdom of Tonga. mBio, 14(1), e03313-22.

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Macroalga S. japonica Growth Under CO2 and Salinity

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S. japonica were grown at two CO₂ concentrations (low CO₂, LC:400 µatm; high CO₂, HC:1000 µatm) and three salinities (30, 25 and 20 psu) for 4 days. Each treatment was carried out in three 500 mL side-arm flasks, and each flask contained 7 thalli with 500 mL freshly collected and sterilized seawater. The two pCO₂ levels were achieved using incubators (GXZ-380C-02, Jingnan Instruments Factory, Ningbo, China) by automatically regulating the fluxes of the ambient air only (for 400 µatm) and combining the gases of air and pure CO₂ (for 1000 µatm). The salinities that were lower than that of the collected seawater (30 psu) were gained by diluting with Milli-Q water. The flasks were placed in incubators with the corresponding pCO₂ under 60 μmol photon m⁻² s⁻¹ (12 h:12 h light/dark cycle) at 9 °C. In order to avoid nutrient depletion, the thalli were cultured in fresh seawater that was enriched with 25% PESI medium [66 (link)]. Before the cultivation, the TA and pH of medium were examined after 24 h of aeration. The pH was measured using a pH meter (Orion STAR A211; Thermo Scientific), and the TA was determined by titration. The carbonate system parameters were calculated using a CO2SYS software [67 ] based on the known temperature, salinity, TA and pH.

Zhang W., Shi Y., He L., Chen X., Hu F., Chen Y., Pang Y., Li S, & Chu Y. (2022). Decreased Salinity Offsets the Stimulation of Elevated pCO2 on Photosynthesis and Synergistically Inhibits the Growth of Juvenile Sporophyte of Saccharina japonica (Laminariaceae, Phaeophyta). Plants, 11(21), 2978.

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Microalgal Biomass Productivity and Nutrient Analysis

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Samples (50–100 mL) were collected at defined time intervals for analysis. First, the pH value was analyzed using a pH meter (Orion Star™ A211, Thermo scientific, USA). The samples were then vacuum-filtered through a pre-weighed GF/C filter paper (47 mm, Whatman, USA) to analyze the biomass based on the procedures in Standards Methods¹⁹. The productivity of microalgal biomass was calculated as follows^{20 (link)}:

P_{Biomass} (g L^{- 1} d^{- 1}) = (C_{2} - C_{1}) / (t_{2} - t_{1})

where P_biomass is the biomass productivity (g L^-1d^-1), C₁ and C₂ are the concentrations of biomass at times t₁ and t₂.
Water analysis was conducted using the filtrates. Filtrates were filtered again using a 0.45 µm polyvinylidene fluoride (PVDF) syringe filter for total nitrogen (TN) and total phosphorus (TP) analysis. The TN values were analyzed using a TN analyzer (TNM-L, Shimadzu, Japan) equipped with a Total organic carbon (TOC) analyzer (TOC-LCPH, Shimadzu, Japan). The TP concentration was analyzed using water test kits (HS-TP-H and HS-TP-L, Humas, Korea) according to the manufacturer’s instructions.

Lee J.C., Moon K., Lee N., Ryu S., Song S.H., Kim Y.J., Lee S.M., Kim H.W, & Joo J.H. (2023). Biodiesel production and simultaneous treatment of domestic and livestock wastewater using indigenous microalgae, Chlorella sorokiniana JD1-1. Scientific Reports, 13, 15190.

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Collagen Fibril Formation Protocol

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Bovine derived atelo-COL1 (Nutragen, Advanced BioMatrix, CA, USA) (416μL) was diluted to a final concentration of 2.5mg/mL⁻¹using 10X PBS (100μL) (Thermo Fisher Scientific, MA, USA), ultrapure water (430μL), and 0.1M NaOH (VWR, PA, USA). The volume of NaOH was adjusted to ensure a pH between 8.8–9.0 to increase the rate of fibril formation. pH measurements were carried out on a 25μL aliquot of the prepared COL1 solution, using a pH probe (Orion Star A211, Thermo Fisher Scientific, USA). All reagents were placed on ice and COL1 was injected within 10 min of neutralization.
Collagen injection was carried out in a sterile biosafety cabinet. Collagen solution was loaded into a 1mL syringe (10148–330, VWR, PA, USA) and the syringe was gently tapped to remove air bubbles. The syringe was loaded into a syringe pump (New Era Pump Systems, NY, USA), for controlling the flow rate. A 20-gauge dispensing elbow needle (Grainger Industrial Supply, IL, USA) was attached to the syringe via a luer-lock and used to inject COL1 at specified flow rates into the channels. A minimum volume of 100μL was injected through the channel to minimize effects of the air-liquid interface. Channels were placed in a petri-dish with a damp lint-free wipe to maintain local humidity and prevent evaporation and transferred to a 37°C incubator to induce COL1 self-assembly.

Otto K., Norbeck J., Larsson T., Karlsson K.A, & Hermansson M. (2001). Adhesion of Type 1-Fimbriated Escherichia coli to Abiotic Surfaces Leads to Altered Composition of Outer Membrane Proteins. Journal of Bacteriology, 183(8), 2445-2453.

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Bacterial Growth Across pH Levels

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LAB were grown at different pH values (2.9, 5.0, 6.4, and 7.4) following the protocol given by Abushelaibi et al. [15 (link)], with some modifications. The strains were grown for 24 h in MRS broth. Each strain was replicated in tubes containing MRS broth with pH adjusted to 2.9, 5.0, 6.4, and 7.4 (pH-meter Orion Star A211, equipped with pH/ATC Ultra Triode probe, Thermo Scientific). The replicated tubes were incubated for 2 h at 37 °C. The absorbance (600 nm) of each tube was measured before and after the incubation. Replicates were performed in triplicates, and the results were expressed as percent growth using the equation (%C).

Pinchao Y.A., Serna-Cock L, & Mora O.O. (2024). Probiotic capacity of commensal lactic acid bacteria from the intestine of Guinea pigs (Cavia porcellus). Heliyon, 10(8), e29431.

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Gelatin Homogenization and pH Measurement

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Five grams of gelatin gel sample (6.67%, w/v) were homogenized with four
volumes of distilled water for 60 s using a homogenizer (UltraTurrax SK15,
Janke & Kunkel, Staufen, Germany; Park et al., 2013 (link)). A pH meter (Orion Star A211, Thermo Fisher
Scientific, Beverly, MA, USA) was calibrated at room temperature using
standard pH buffers (pH 4.01, 7.00, and 10.01).

Ham Y.K., Noh S.W., Lee J.H., Yang N.E., Choi Y.S, & Kim H.W. (2023). Optimization of Gelatin Extracting Condition from Korean Native Black Goat Skin and Quality Comparison with Commercial Gelatin. Food Science of Animal Resources, 43(1), 61-72.

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pH Determination of Yeast Cultures

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pH determination of YC samples was performed according to Zaika et al. [1 (link)] with slight modification. Briefly, 20 g of YC samples were added to 160 mL of distilled water and mixed thoroughly. The solution was filtered, and 50 mL of this solution was further diluted 1:1 with 50 mL of distilled water. The pH was measured using a digital pH meter (Orion Star A211, ThermoFisher Scientific, Waltham, MA, USA) by immersing the electrode into the solution.

Pakroo S., Tarrah A., da Silva Duarte V., Corich V, & Giacomini A. (2020). Microbial Diversity and Nutritional Properties of Persian “Yellow Curd” (Kashk zard), a Promising Functional Fermented Food. Microorganisms, 8(11), 1658.

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Evaluating Emulsion Stability Through pH Monitoring

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Since a changing pH value indicates unstable dissolution of the solute in the solvent, the stability of the emulsions can be indirectly determined by performing pH measurements. Unstable pH can also result in inconsistent drug efficacy and discomfort to the patient during administration of the drug. [17 (link)] Therefore, drug pH was monitored 30 min after brief vortexing at 25°C and 35°C using a benchtop pH meter (Orion Star A211; Thermo Fisher Scientific, Waltham, MA, USA). The pH value of each CsA emulsion was read every minute.

Bang S.P., Yeon C.Y., Adhikari N., Neupane S., Kim H., Lee D.C., Son M.J., Lee H.G., Kim J.Y, & Jun J.H. (2019). Cyclosporine A eyedrops with self-nanoemulsifying drug delivery systems have improved physicochemical properties and efficacy against dry eye disease in a murine dry eye model. PLoS ONE, 14(11), e0224805.

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