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Cd15 bv785 clone w6d3

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CD15-BV785 (clone W6D3) is a fluorochrome-conjugated antibody that binds to the CD15 antigen expressed on the surface of certain cell types. This product is suitable for flow cytometry applications.

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Lab products found in correlation

Cd34 pe clone 563, by BD (2 mentions) Cd45 apc clone hi30, by BD (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Fortessa, by BD (2 mentions) Mcd45 pe cy7 clone 30 f11, by BD (1 mentions) Facsaria 2, by BD (1 mentions) Cd19 pe clone hib19, by BD (1 mentions) Cd33 bv421 clone wm53, by BD (1 mentions) Cd38 pe cf594 clone hit2, by BD (1 mentions) Cd14 apc clone m5e2, by BD (1 mentions)

2 protocols using cd15 bv785 clone w6d3

1

Comprehensive Immune Cell Profiling

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The following antibodies were used: CD34-PE (clone 563, BD Pharmingen), CD45-APC (clone HI30, BD PharMingen), CD33-BV421 (clone WM53, BD Horizon), mCD45-PE-Cy7 (clone 30-F11, BD Biosciences), CD19-PE (clone HIB19, BD Pharmingen), CD38-PE-CF594 (clone HIT2, BD Horizon), CD15-BV785 (clone W6D3, BioLegend), CD14-APC (clone M5E2, BD Horizon), and CD44-APC. Cell viability was assessed with DAPI (Life Technologies). Cells were assayed on a BD Fortessa and data were analyzed with FlowJo software (Tree Star). Cell sorting was performed on a BD FACSAria II.
Kotini A.G., Carcamo S., Cruz-Rodriguez N., Olszewska M., Wang T., Demircioglu D., Chang C.J., Bernard E., Chao M.P., Majeti R., Luo H., Kharas M.G., Hasson D, & Papapetrou E.P. (2023). Patient-Derived iPSCs Faithfully Represent the Genetic Diversity and Cellular Architecture of Human Acute Myeloid Leukemia. Blood Cancer Discovery, 4(4), 318-335.
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2

Flow Cytometry Immunophenotyping Protocol

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The following antibodies were used: CD34-PE (clone 563, BD Pharmingen, RRID:AB_393871), CD45-APC (clone HI30, BD Pharmingen, RRID:AB_398600), CD14-APC (clone M5E2, BD Pharmingen, RRID:AB_398596), CD15-BV785 (clone W6D3, BioLegend, RRID:AB_2632921), CD16-BV510 (clone 3G8, BD Horizon, RRID:AB_2744296). Cell viability was assessed with DAPI (Life Technologies). Cells were assayed on a BD Fortessa and data were analyzed with FlowJo software (Tree Star, RRID:SCR_008520).
Wheeler E.C., Vora S., Mayer D., Kotini A.G., Olszewska M., Park S.S., Guccione E., Teruya-Feldstein J., Silverman L., Sunahara R.K., Yeo G.W, & Papapetrou E.P. (2022). Integrative RNA-omics discovers GNAS alternative splicing as a phenotypic driver of splicing factor-mutant neoplasms. Cancer discovery, 12(3), 836-855.
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