Ncbp1

The protein was extracted with western blot (WB) lysis buffer (BMP2020, Abbkine) containing PMSF and quantified with a BCA kit (SW101‐02, Severn Biotech). An equal amount (20 μg) of protein extracts was separated on 10% SDS‐PAGE gels then transferred to PVDF membranes (Immobilon‐P, Millipore). The cell membrane was treated with the appropriate initial antibodies at 4°C overnight after being blocked in 5% non‐fat milk for 1.5 h. After washing three times with TBST for 10 min, the membranes were incubated with the secondary antibody (1: 5000 dilution; Biosharp, China) for 2 h at room temperature. After washing with TBST for half an hour, the signals were visualized using ECL reagent (BMU102‐CN, Abbkine, China) and quantified using Image Lab Software (Bio‐Rad). Primary antibodies were listed as follows: EIF4E (AB33766, Abcam), NCBP1 (10349‐1‐AP, Proteintech), WDR4 (PAB40170, Bioswamp), GAPDH (BM1623, Boster Biological Technology Co. ltd).