Inferior nasal turbinate swabs were collected and placed in 1 ml of Zymo-DNA/RNA shield reagent (Zymo Research). RNA was extracted from 250 μL of sample and eluted into a volume of 50ul using the KingFisher Flex sample purification system (ThermoFisher) and the quick DNA-RNA MagBead kit (Zymo Research) following the manufacturer’s instructions. Each sample was extracted twice in parallel. The 2 eluted RNA samples were pooled and aliquoted into 20 μL aliquots using a Rainin Liquidator 96 pipettor for downstream RT-qPCR, RNA-sequencing, and viral sequencing.
Kingfisher flex sample purification system
Manufactured by Thermo Fisher Scientific
The KingFisher Flex sample purification system is a versatile automated platform designed for high-throughput sample preparation. It utilizes magnetic separation technology to efficiently capture, wash, and elute target analytes from a variety of sample types. The system is capable of processing multiple samples simultaneously, making it a suitable solution for diverse applications that require consistent and reproducible sample preparation.
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Lab products found in correlation
3 protocols using kingfisher flex sample purification system
1
Nasal Swab RNA Extraction and Aliquoting
2
Nasal Swab RNA Extraction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Inferior nasal turbinate swabs were collected and placed in 1mL of Zymo-DNA/RNA shield reagent (Zymo Research). RNA was extracted from 250 μL of sample and eluted into a volume of 50ul using the KingFisher Flex sample purification system (ThermoFisher) and the quick DNA-RNA MagBead kit (Zymo Research) following the manufacturer’s instructions. Each sample was extracted twice in parallel. The 2 eluted RNA samples were pooled and aliquoted into 20 μL aliquots using a Rainin Liquidator 96 pipettor for downstream RT-qPCR, RNA-sequencing, and viral sequencing.
3
Nasal Swab RNA Extraction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Inferior nasal turbinate swabs were collected and placed in 1ml of Zymo-DNA/RNA shield reagent (Zymo Research). RNA was extracted from 250 μL of sample and eluted into a volume of 50ul using the KingFisher Flex sample purification system (ThermoFisher) and the quick DNA-RNA MagBead kit (Zymo Research) following the manufacturer’s instructions. Each sample was extracted twice in parallel. The 2 eluted RNA samples were pooled and aliquoted into 20 μL aliquots using a Rainin Liquidator 96 pipettor for downstream RT-qPCR, RNA-sequencing, and viral sequencing.
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