Cells were grown on 35-mm glass-bottom dishes in phenol red-free DMEM containing 10% FBS. Cells were subjected to long-term, time-lapse imaging using a computer-assisted fluorescence microscope (Olympus, LCV100) equipped with an objective lens (Olympus, UAPO 40×/340 N.A. = 0.90), a halogen lamp, a red LED (620 nm), a CMOS camera (Hamamatsu Photonics, ORCA-Flash4.0), differential interference contrast (DIC) optical components, and interference filters. The halogen lamp was used with BrightLine® single-band filter set (Semrock): “FITC-2024B” for observing the h2-3 fluorescence, and “mCherry-C” for observing the AxaleaB5 fluorescence. For DIC imaging, the red LED was used with a filter cube containing an analyzer. Image acquisition and analysis were performed using MetaMorph 6.37 and 7.10 software (Molecular Devices), respectively. See Table II for details.
Brightline single band filter set
Manufactured by IDEX Corporation
The BrightLine® single-band filter set is a collection of optical filters designed for laboratory equipment. The filters are engineered to selectively transmit a specific wavelength of light while blocking all other wavelengths. This core function allows for precise isolation and measurement of the desired spectral component in various scientific and analytical applications.
Automatically generated - may contain errors
2 protocols using brightline single band filter set
1
Time-lapse Imaging of Fluorescent Proteins
2
Time-lapse Imaging of Fluorescent Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were grown on 35-mm glass-bottom dishes in phenol red-free DMEM containing 10% FBS. Cells were subjected to long-term, time-lapse imaging using a computerassisted fluorescence microscope (Olympus, LCV100) equipped with an objective lens (Olympus, UAPO 40×/340 N.A. = 0.90), a halogen lamp, a red LED (620 nm), a CMOS camera (Hamamatsu Photonics, ORCA-Flash4.0), differential interference contrast (DIC) optical components, and interference filters. The halogen lamp was used with BrightLine® single-band filter set (Semrock): "FITC-2024B" for observing the h2-3 fluorescence, and "mCherry-C" for observing the AxaleaB5 fluorescence. For DIC imaging, the red LED was used with a filter cube containing an analyzer. Image acquisition and analysis were performed using MetaMorph 6.37 and 7.10 software (Molecular Devices), respectively. See Table 2 for details.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!