3 tert butyl 4 hydroxyanisole bha

All the reagents were purchased from Sigma Chemical (St. Louis, MO), including dimethyl sulfoxide (DMSO), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyl, tetrazolium bromide (MTT), 3-tert-butyl-4-hydroxyanisole (BHA), ethylenediaminetetraacetic acid (EDTA), FeCl₃, FeCl₂·4H₂O, kojic acid, L-tyrosine, mushroom tyrosinase, potassium ferricyanide [K₃Fe(CN)₆], trichloroacetic acid and vitamin C, and other highest purity chemical buffers and reagents. Cell culture reagents were purchased from GIBCO BRL (Gaithersburg, MD), including fetal bovine serum (FBS) and Dulbecco's modified Eagle medium (DMEM).

Green soybeans were obtained from a local farm (Harbin, China). Salt, paprika, and ginger powder were purchased from a supermarket (Beijing, China). B. velezensis CAU263 (CGMCC NO: 20318) isolated from Chinese traditional douchi was used in this study. Fibrinogen from bovine plasma, thrombin, DPPH, ABTS, angiotensin I‐converting enzyme (ACE), 2,4,6‐tris (2‐pyridyl)‐s‐triazine (TPTZ), (±)‐6‐hydroxy‐2,5,7,8‐tetramethyl‐chromane‐2‐carboxylic acid (trolox), 3‐tert‐butyl‐4‐hydroxyanisole (BHA), and hippuryl‐L‐histidyl‐L‐leucine (HHL) were obtained from Sigma‐Aldrich Co. Ltd. Peptone and yeast extract were obtained from Oxoid Co. Ltd. Rutin was gained from Shanghai Yuanye Biotechnology Co. Ltd. Gallic acid was obtained from Shanghai Macklin Biochemical Co. Ltd. L (+)‐ascorbic acid (VC) was acquired from Fujifilm Wako Pure Chemical Corporation. Other chemical reagents were obtained from Beijing Chemical Works.