E. coli Stbl3 transformed with pZsGreen-N1 (Clontech laboratories) that encodes ZsGreen, a green fluorescent protein (GFP), were grown in LB medium and the plasmid was purified by PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen), following the manufacturer’s instructions and quantified by NanoDrop-OneC-UV-Vis (Thermo Fisher).
Nanodrop onec uv vis
Manufactured by Thermo Fisher Scientific
The NanoDrop-OneC-UV-Vis is a laboratory instrument designed for the quantification and analysis of nucleic acids and proteins. It utilizes UV-Vis spectroscopy to measure sample concentrations and purity. The instrument requires only a small sample volume, typically 1-2 μL, to perform these measurements.
Automatically generated - may contain errors
2 protocols using nanodrop onec uv vis
1
Purification of pZsGreen-N1 Plasmid
2
Bacterial Plasmid Preparation and Quantification
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E. coli Stbl3 transformed with pZsGreen-N1 (Clontech laboratories, no. 632448) encoding ZsGreen, a green fluorescent protein (GFP), or recombinant HexaPro Spike plasmid (Addgene, no. 154754) (Supplementary Fig. 2 ) were grown in LB medium (Sigma-Aldrich, no. L3022) containing kanamycin (50 μg/ml) or ampicillin (100 μg/mL), respectively, at 37 °C for 16 h. The plasmids were purified by PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen, no. K210007), following the manufacturer’s instructions, and quantified using a NanoDrop-OneC-UV-Vis (ThermoFisher, no. ND-ONE-W).
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