Mouse anti β actin mab

PEDV-permissive Vero cells and human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific) and 100 U/mL of penicillin plus 100 g/mL streptomycin. IPEC-DQ cells were cultured in Roswell Park Memorial Institute (RPMI) medium (Hyclone Laboratories, Logan, UT, USA) containing 10% FBS with penicillin/streptomycin. All cells were maintained at 37 °C with 5% CO₂ in a humidified incubator. The PEDV strain used in this study was a mutant strain of PEDV AJ1102 (GenBank: JX188454.1). Poly(I:C)-LMW, poly(I:C)-HMW, 2′3′-cGAMP and poly(dA:dT) were all obtained from InvivoGen (Hongkong, China). Mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mAb, mouse anti-green fluorescence protein (GFP) mAb, and mouse anti-β-Actin mAb were all brought from TransGen (Beijing, China). Mouse anti-MAVS mAb was from Santa Cruz Biotechnology (Dallas, TX, USA), rabbit anti-TRIF pAb was from Biodargon (Beijing, China), and rabbit anti-STING pAb was bought from Proteintech (Wuhan, China).

Baby hamster kidney cells (BHK-21) were obtained from the American Type Culture Collection (ATCC) and were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco Life Technologies, shanghai, China) supplemented with 10% fetal bovine serum (FBS; Gibco Life Technologies, shanghai, China) in 5% CO₂ at 37°C.
Mouse anti-TMUV serum was produced by our laboratory previously (Chen et al., 2018a (link)). Antibody against TMUV E mAb was produced by our laboratory. Rabbit anti-Calnexin pAb was obtained from Abcam (ab22595). Mouse anti-β-actin mAb was obtained from Transgen. Goat anti-mouse IgG [horseradish peroxidase (HRP), fluorescein isothiocyanate (FITC)] used as the secondary antibody was obtained from Thermo Fisher Scientific (USA).