Blood was drawn in 3.2 % sodium citrate tubes for frozen plasma samples, in Tempus Blood RNA tubes (ABI) for genome-wide RNA profiling, and in BD Vacutainer K2 tubes for complete blood counts with differentials.
Vacutainer k2
Manufactured by BD
Sourced in United States
The BD Vacutainer K2 is a blood collection tube that contains the anticoagulant K2 EDTA. It is used to collect and store blood samples for various laboratory tests.
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Lab products found in correlation
6 protocols using vacutainer k2
1
Blood Sample Collection Protocol
2
Dried Blood Characterization for MRSA and E. coli Detection
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Whole venous blood samples were drawn with a syringe from healthy, consenting MRSA- and E. coli-negative adult volunteers; samples were later transferred to 6-mL BD Vacutainer K2 Ethylenediaminetetraacetic acid (EDTA) collection tubes. The tubes were stored in a sample rotisserie at 4 °C before using them for experiments.
Tenfold serial blood dilutions of DNA or bacterial stocks were done to achieve the correct concentration required for experimentation. The spiked blood was then distributed into 0.2-mL PCR tubes (4 µL in each tube). This blood was dried on a hot plate at 37 °C for 20 min.
For blood drying characterizations, we tested different drying conditions, which can be found inSI Appendix, Table S1 . Samples of the dried blood before and after thermal lysis were prepared, and SEM analysis was performed to quantify the porosity of the blood matrices. The details of the blood drying temperature, time, and corresponding porosity before and after thermal lysis are summarized in SI Appendix, Table S1 .
Tenfold serial blood dilutions of DNA or bacterial stocks were done to achieve the correct concentration required for experimentation. The spiked blood was then distributed into 0.2-mL PCR tubes (4 µL in each tube). This blood was dried on a hot plate at 37 °C for 20 min.
For blood drying characterizations, we tested different drying conditions, which can be found in
3
Blood Sample Centrifugation and Plasma Collection
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Ten-milliliter K2- (BD vacutainer K2, 367525, BD, Franklin Lakes, NJ) or K3-EDTA tubes (455036, Greiner Bio-One GmbH, Kremsmunster, Austria) were used for the blood draw. After blood collection, the blood collection tube was gently inverted 10 times immediately. Blood collection tubes were immediately centrifuged at 1,500–2,500 g for 15 min within 1 h. In this study, each participant was requested to donate two tubes of blood. One was centrifuged at RT, the other was centrifuged at 4°C. A swing-out (basket) rotor was used for centrifugation. By using a disposable 1 ml micropipette tip, every 1 ml of plasma (supernatant) was transferred to a fresh 1.5 ml Eppendorf tube. All plasma samples were frozen at –80°C before measurements.
4
Plasma cfDNA Extraction and Germline DNA Isolation
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Blood samples were collected in up to 4 × 10 mL Cell-Free DNA BCT tubes (Streck, Omaha, NE), for cfDNA analysis. Plasma was separated from whole blood within 96 h of blood draw by two sequential centrifugations (2,000 g, 10 min) and stored at −80 °C before cfDNA processing. In addition, 1 × 10 mL BD Vacutainer K2 ethylenediaminetetraacetic acid (K2EDTA) sample was taken for germline DNA extraction according to study protocols.
5
Fasting Glucose and Lipid Profiling
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Following a 10 – 12 hr overnight fast, whole blood samples were collected from the antecubital vein into a BD Vacutainer® K2 (Becton, Dickinson & Co., Franklin Lakes, New Jersey, United States) containing ethylenediaminetetraacetic acid or BD Vacutainer® Serum separator tubes with separator gel. The samples were kept at room temperature to allow clotting. The serum fraction was recovered and frozen at −20 °C until use. Samples were used for the following endpoints: fasting plasma glucose, insulin, glycated hemoglobin (HbA1c), total cholesterol, high-density lipoprotein, low-density lipoprotein, and triglycerides. An additional blood sample was obtained 2 hours after oral glucose administration (75 g) to determine tolerance. Fasting plasma glucose and oral glucose tolerance were determined, in duplicate, using the enzymatic method/spectrophotometric glucose oxidation (Beckman Coulter Inc., Brea, California, United States). The HbA1c levels were determined by the turbidimetric inhibition immunoassay. Insulin levels were determined by an automated immunoassay (Beckman Coulter Inc.). To determine total cholesterol, high-density lipoprotein, low-density lipoprotein, and triglycerides, serum samples were sent to the Central Laboratory of the Multidisciplinary Research Group, which is part of IMSS.
6
Blood Sample Aliquoting and Storage
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Whole blood is collected from 6 mL BD Vacutainer® K2 Ethylene Diamine Tetra Acetic Acid (EDTA) tubes (Cat No. 367873, Becton Dickinson, East Rutherford, NJ, USA). A maximum of 2 × 1 mL aliquots are stored in FluidX tubes (Cat No. 68-1003-11, BioTools, Keperra, QLD, Australia). Aliquots are stored at −80 °C until requested for analysis by sub-projects.
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