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Model 315c

Manufactured by Aurora Scientific
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Sourced in Canada

The Model 315C is a precision force transducer designed for scientific and industrial applications. It features a high-resolution strain gauge sensor that measures force with accuracy and repeatability. The device's compact size and rugged construction make it suitable for a variety of laboratory and test environments.

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Lab products found in correlation

Aluminum t clips, by Aurora Scientific (2 mentions) Model 400a, by Aurora Scientific (2 mentions) Model 403a, by Aurora Scientific (2 mentions) Danicamtiv, by MedChemExpress (1 mentions) Brij 58, by Merck Group (1 mentions)

Spelling variants of this product

Model 315C-I (2 citations)

4 protocols using model 315c

1

Cardiac Muscle Contractility Measurements

Cited 1 time
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Permeabilized trabeculae (mouse) or thin strips (pig) were dissected and mounted between a force transducer (Aurora Scientific, model 400A) and a motor (Aurora Scientific, model 315C) using aluminum T-clips (Aurora Scientific).14 (link) Sarcomere length (SL) was set to ~2.3 μm for the experiments. Experiments were conducted in physiological solution (pH 7.0) at 15°C (mouse) or 21°C (pig) containing a range of pCa (= −log10[Ca2+]) values from 9.0 to 4.0 with and without 1μM Dani (MedChemExpress). Tissue was allowed to reach stead-state force (F) at each pCa. F-pCa curves were collected and analyzed with custom code using LabView software and fit to the Hill equation. The rate of tension redevelopment (ktr) and high frequency stiffness (HFS) were measured at each pCa (details in the supplement).
Keller C.P, & Van Volkenburgh E. (1998). Evidence That Auxin-Induced Growth of Tobacco Leaf Tissues Does Not Involve Cell Wall Acidification. Plant Physiology, 118(2), 557-564.
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2

Contractile Properties of Cardiac Muscle

Cited 1 time
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Permeabilized trabeculae (mouse) or thin strips (pig) were dissected and mounted between a force transducer (Aurora Scientific, model 400A) and a motor (Aurora Scientific, model 315C) using aluminum T-clips (Aurora Scientific).14 (link) Sarcomere length was set to ≈2.3 µm for the experiments. Experiments were conducted in physiological solution (pH 7.0) at 15 °C (mouse) or 21 °C (pig) containing a range of negative log of calcium concentration (pCa; −log10[Ca2+]) values from 9.0 to 4.0 with and without 1 μM Danicamtiv (MedChemExpress). Tissue was allowed to reach stead-state force (F) at each pCa. F-pCa curves were collected and analyzed with custom code using LabView software and fit to the Hill equation. The rate of tension redevelopment (ktr) and high-frequency stiffness was measured at each pCa (details in the Supplemental Material).
Kooiker K.B., Mohran S., Turner K.L., Ma W., Martinson A., Flint G., Qi L., Gao C., Zheng Y., McMillen T.S., Mandrycky C., Mahoney-Schaefer M., Freeman J.C., Costales Arenas E.G., Tu A.Y., Irving T.C., Geeves M.A., Tanner B.C., Regnier M., Davis J, & Moussavi-Harami F. (2023). Danicamtiv Increases Myosin Recruitment and Alters Cross-Bridge Cycling in Cardiac Muscle. Circulation Research, 133(5), 430-443.
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3

Measuring Single Muscle Fiber Dimensions

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Single fibre dimensions were determined as previously described [35 (link)]. Briefly, the skinned fibre bundles were placed in relaxing Brij solution containing 0.5% Brij-58 (polyoxyethylene 20 cetyl ether; Sigma-Aldrich, St. Louis, MO) for 30 min at 4°C. Then, the bundles were transferred to relaxing solution for the isolation of single fibres. Each fibre was transferred to an experimental apparatus (1400A Permeabilized Fiber Test System, Aurora Scientific Inc., Ontario, Canada) and mounted on the stage of an inverted microscope (Olympus IX71, Tokyo, Japan). The fibre was attached between connectors leading to a force transducer (Model 403A, Aurora Scientific Inc., ON, Canada) and a direct-current torque motor (Model 315C, Aurora Scientific, ON, Canada). Immediately, the fibre segment was submerged in relaxing solution at 15°C to proceed with morphological measurements. The sarcomere length (set to 2.75-2.85 μm) and the fibre's diameter, depth, and length were measured using the Video Sarcomere Length Image Analysis System (Aurora Scientific Inc., ON, Canada). Fibre cross-sectional area (CSA) was calculated using width and depth of the fibre, assuming an elliptical circumference and corrected for the 20% swelling that follows skinning [36 (link), 37 (link)].
Javadov S., Jang S., Rodriguez-Reyes N., Rodriguez-Zayas A.E., Hernandez J.S., Krainz T., Wipf P, & Frontera W. (2015). Mitochondria-targeted antioxidant preserves contractile properties and mitochondrial function of skeletal muscle in aged rats. Oncotarget, 6(37), 39469-39481.
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4

Calcium-Dependent Isometric Tension in Permeabilized Myocytes

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Calcium-dependent isometric tension was measured in permeabilized non-treated controls and myocytes expressing cTnI and cTnIS45D after 4 days post-gene transfer. Individual myocytes were mounted to a piezoelectric motor (Model 315C, Aurora Scientific, Aurora,ON) and a force transducer (Model 403A, Aurora Scientific) in high relaxing (HR) buffer composed of pCa (−log [Ca2+]) 9.0, 10 mM EGTA, 20 mM Imidazole, 1 mM free Mg2+, 4 mM free ATP, 14.5 mM creatine phosphate and sufficient KCl for an ionic strength of 180 mM at 15°C. Ion concentrations for each pCa were calculated using MATLAB [39 (link)]. Myocytes were permeabilized in HR with 0.1% Triton X-100, sarcomere length (SL) was set to 2.0 or 2.3 μm, and active tension measured over a pCa (−log [Ca2+]) range of 9.0 to 4.5 using the slack test approach [29 (link)]. Tension-pCa curves were fit to the Marquardt-Levenburg non-linear, least squares algorithm for the Hill equation, where P is the fractional tension, K is the midpoint or –log [Ca2+] producing 50% peak tension (pCa50) and nH is the Hill coefficient in the equation: P=[Ca2+]nH∕(KnH+[Ca2+]nH).
Lang S.E., Schwank J., Stevenson T.K., Jensen M.A, & Westfall M.V. (2014). Independent Modulation of Contractile Performance by Cardiac Troponin I Ser43 and Ser45 in the Dynamic Sarcomere. Journal of molecular and cellular cardiology, 79, 264-274.
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