Waters acquity uplc

Manufactured by Waters Corporation

Sourced in United States, France, United Kingdom, Italy

The Waters Acquity UPLC is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It utilizes ultra-high pressure liquid chromatography (UPLC) technology to achieve enhanced separation efficiency, sensitivity, and speed compared to traditional HPLC systems.

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Acquity UPLC (716 citations) Waters Acquity UPLC system (131 citations) Waters Acquity UPLC H-Class system (15 citations) Waters Acquity UPLC BEH C18 (14 citations) Waters Acquity UPLC HSS T3 column (13 citations) Waters Acquity I-Class UPLC (6 citations) Waters Acquity UPLC CSH Phenyl Hexyl column (4 citations) Waters ACQUITY UPLC-Xevo TQ-S (3 citations) Waters ACQUITY-UPLC CLASS system (3 citations) Waters Acquity UPLC Shield RP18 column (2 citations)

80 protocols using waters acquity uplc

Triple Quadrupole Mass Spectrometry Protocols

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Two different triple quadrupole instruments (QqQ) operated in MS/MS mode were used:
 Waters Acquity UPLC TM (Waters Corp.), equipped with a quaternary pump system, interfaced to triple quadrupole mass spectrometer TQD TM with orthogonal (ESI) Z-spray (Waters Corp.).
 Waters Acquity UPLC TM (Waters Corp.), equipped with a binary pump system, interfaced to triple quadrupole mass spectrometer Xevo TQ-S TM (Waters Corp.) equipped with TWave devices and an orthogonal ESI source.
For further details, see Supplementary Information.

Pitarch E., Cervera M.I., Portolés T., Ibáñez M., Barreda M., Renau-Pruñonosa A., Morell I., López F., Albarrán F, & Hernández F. (2016). Comprehensive monitoring of organic micro-pollutants in surface and groundwater in the surrounding of a solid-waste treatment plant of Castellón, Spain. The Science of the total environment, 548-549.

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Quantification of Analytes in DBS by UPLC-MS/MS

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Analytes under investigation in DBS were detected using an ultra-high performance liquid chromatography system (Waters Acquity UPLC, Waters Corporation, Milan, Italy) coupled with a triple quadrupole mass spectrometer (Waters Xevo TQ, Waters Corporation). Chromatography was carried out in reversed phase Acquity UPLC HSS C18 column (2.1 mm × 150 mm, 1.8 μm, Waters
Acquity UPLC, Waters Corporation, Milan, Italy) using a linear gradient elution with two solvents: 0.1% formic acid in acetonitrile (solvent A) and 5 mM ammonium formate pH 3.0 (solvent B).
Solvent A was maintained at 5% for the first 0.50 min. It was increased to 55% from 0.50 to 10.00 min, then increased to 90% from 10.00 to 10.75 min, held at 90% from 10.75 to 12.85 min, and then decreased back to 5% from 12.85 to 13.00 min and held at 5% from 13.00 to 1.

Kyriakou C., Marchei E., Scaravelli G., García-Algar O., Supervía A, & Graziano S. (2016). Identification and quantification of psychoactive drugs in whole blood using dried blood spot (DBS) by ultra-performance liquid chromatography tandem mass spectrometry. Journal of pharmaceutical and biomedical analysis, 128.

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UPLC-MS Analysis of Polyphenols

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One hundred micrograms of internal standard (methyl 4-hydroxybenzoate) was added to an aliquot of 4 ml of the polyphenol extract, which was concentrated to dryness by speed vac at 45ºC. After the addition of 1 ml of the mixture of MeOH/formic acid (95:5 v/v), the solubilized polyphenolic extract was filtered (RC 0.2 µm Phenomenex, Le Pecq, France), and 1 µL was directly injected into a Waters Acquity UPLC equipped with a photodiode array detector (280, 330, and 520 nm) and a Waters Acquity HSS T3 × 8 μ by W which was housed in a 45°C heated oven. The identification of the phenolic compounds was also achieved through the coupling of a Waters Acquity UPLC with an ion trap mass spectrometer Bruker Daltonics HCT ultra equipped with an electrospray ionization source. Details about the separation, characterization and quantification of phenolic compounds were previously described by Aubert et al. (Aubert et al., 2014) .

Saidani F., Giménez R., Aubert C., Chalot G., Betrán J.A, & Gogorcena Y. (2017). Phenolic, sugar and acid profiles and the antioxidant composition in the peel and pulp of peach fruits. Journal of food composition and analysis : an official publication of the United Nations University, International Network of Food Data Systems, 62.

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Quantification of Metformin and Dapagliflozin in Plasma

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Human plasma concentrations of metformin HCl and dapagliflozin l-proline were determined using LC–MS/MS methods. To measure the concentrations of metformin HCl, the LC–MS/MS system consisted of a Micromass Quattro micro API (Waters) coupled with a Waters ACQUITY UPLCTM (Waters). Samples were prepared by protein precipitation with acetonitrile. Chromatographic separation was achieved on an ACQUITY UPLC^® BEH HILIC Silica (2.1 mm × 50 mm, 1.7 μm) (Waters) with an isocratic solvent system. Using electron spray ionization in the positive ion mode with mass transitions, the mass spectrometer was operated at m/z 130.1→59.9 for metformin HCl and m/z 136.2→59.9 for metformin HCl-d₆ (IS). The calibration range of metformin HCl in plasma was 20–5000 ng/mL. The process parameters to determine dapagliflozin l-proline concentrations were the same as those for metformin HCl. Using electron spray ionization in the positive ion mode with mass transitions, the mass spectrometer was operated at m/z 426.2→167.1 for dapagliflozin l-proline and m/z 431.2→167.1 for dapagliflozin l-proline-d₅ (IS). The calibration range of dapagliflozin l-proline in the plasma was 1–400 ng/mL.

Chun M.H., Kim J.Y., Park E.S, & Choi D.H. (2021). Development of a Robust Control Strategy for Fixed-Dose Combination Bilayer Tablets with Integrated Quality by Design, Statistical, and Process Analytical Technology Approach. Pharmaceutics, 13(9), 1443.

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Quantitative Bile Acid Analysis by UPLC-MS/MS

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A total of 66 bile acids (Supplementary Table S2) were scanned and quantified on a Waters Acquity UPLC (Waters Corp., Milford, United States) coupled with a Sciex 5500+ triple quadrupole (QQQ) mass spectrometer (AB Sciex, Singapore). The bile acids were chromatographically resolved on an C18-PFP column (3 μm, 2.1 × 50 mm; ACE, United Kingdom) after 2.5 μl aliquots of bile acid extract was injected. Water containing 2 mM ammonium acetate was used as phase A, and acetonitrile was used as phase B. The chromatographic gradient ramped from 17% phase B to 30% in 10 min, ramped to 55% in 3 min, rapidly climbed to 95% in 1 min and remained for 3 min; another 5 min was used for column washing and equilibration. The flow rate was set at 0.4 ml min⁻¹. The metabolites were ionized by a TurboV heated electrospray ionization source and then detected by scheduled multiple reaction monitoring mode. The main parameters were optimized as follows: negative ion spray voltage was −4.5 kV, curtain gas pressure was 35 psi, ion gas 1 and 2 pressure were 50 psi, and heater temperature was 550°C.

Deng D., Pan C., Wu Z., Sun Y., Liu C., Xiang H., Yin P, & Shang D. (2021). An Integrated Metabolomic Study of Osteoporosis: Discovery and Quantification of Hyocholic Acids as Candidate Markers. Frontiers in Pharmacology, 12, 725341.

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UPLC Quantification of Compound A

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Example 9

About 5 mg of microspheres are dissolved in 10 mL of a 1:2 v/v dichloromethane/acetonitrile mixture. 1 mL of the solution is transferred into 1.5 mL microtube and solvent is removed using the Savant Speed Vac; then the residue is reconstituted in 95% acetonitrile/5% water. This solution is filtered (Waters 0.2 μm Nylon syringe filter) and measurements are conducted in triplicate via Waters Acquity UPLC with a PDA UV absorbance detector set at 314 nm. The mobile phase is gradient acetonitrile-water with v/v 0.1% formic acid. Waters Acquity UPLC HSS T3 (2.1×50 mm) column is used with the flow rate at 0.3 ml/min. A standard curve is prepared to include about 0.1-0.7 mg/mL of the expected Compound A concentration.

Drug loading is determined as: percent drug loading=100×(10× concentration of filtered solution)/weight of microspheres used. The results are reported as the mean±SD.

US10960010B2. Pharmaceutical compositions for sustained or delayed release (2021-03-30). INTRA-CELLULAR THERAPIES, INC. [US]. Inventors: Kimberly Vanover [US], Peng Li [US], Sharon Mates [US], Robert Davis [US], Lawrence P. Wennogle [US].

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UPLC-MS/MS Analysis of Triacetylfusarinine C

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UPLC-MS/MS analysis was performed using Waters Acquity UPLC with tandem Waters Acquity PDA and TQD detectors (Waters Limited, Ontario, Canada). Separation of TAFC was done using the Waters Acquity BEH reverse phase C18 column (1.7 μm, 2.1 mm x 50 mm). Mobile phase A consisted of 0.1% formic acid in water while mobile phase B was 0.1% formic acid in acetonitrile. A gradient separation, using 10%–90% mobile phase B was performed at a flow rate of 0.3 ml/min. The injection volume was 5 μL and the column temperature was set at 30°C. The eluted TAFC was injected directly into the tandem quadrupole mass spectrometer operated in the positive electrospray ionization (ESI+) mode with a capillary voltage of 0.50 kV with nitrogen gas at a temperature of 400°C. Data was acquired in the multiple reaction monitoring (MRM) mode and Waters Empower chromatography software was used for control of the equipment and data acquisition (Waters Limited, Ontario, Canada). TAFC product ions were extracted with a span of 0.1Da.

Carroll C.S., Amankwa L.N., Pinto L.J., Fuller J.D, & Moore M.M. (2016). Detection of a Serum Siderophore by LC-MS/MS as a Potential Biomarker of Invasive Aspergillosis. PLoS ONE, 11(3), e0151260.

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Analytical Protocol for Estrogens and Nonylphenols

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Both estrogens and nonylphenols were analyzed as described elsewhere [33 (link),34 (link)]. Briefly, for estrogens 1-L samples of settled final effluent were filtered through 1.2 μm GF/C filter paper (VWR, Lutterworth, UK); for nonylphenols, samples (250 mL of final effluent or 100 mL of settled sewage) were filtered through 1.2 μm GF/C filter paper (VWR, Lutterworth, UK). In both cases, filtrates were put through solid phase extraction cartridges and detection and quantification of target analytes were done by UPLC-MS/MS (Waters Acquity UPLC, Waters, Manchester, UK). The instrument was operated under multiple reaction monitoring (MRM) mode; fragmentation reactions and instrumental parameters are included in the supporting information.

Mensah L., Petrie B., Scrimshaw M., Cartmell E., Fletton M, & Campo P. (2023). Influence of solids and hydraulic retention times on microbial diversity and removal of estrogens and nonylphenols in a pilot-scale activated sludge plant. Heliyon, 9(9), e19461.

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Fenofibrate Pharmacokinetics in Mice

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Liver, brain, and plasma samples from C57BL/6J male mice treated for 1 or 8 days with fenofibrate (150 mg/kg; n=6 per group) were collected 2 hours after the final injection and sent to inVentiv Health Clinical Lab, Inc. (Princeton, NJ) for mass spectrometry analysis (LC-MS/MS) to measure levels of fenofibrate and its active metabolite, fenofibric acid. The parent compound, fenofibrate, was not observed at the detection limit of the bioanalytical assay. One part of plasma, brain, or liver was homogenized with 4 parts of lysate in a FastPrep (MP Biomedicals, Santa Ana, CA) homogenizer. After protein precipitation, the samples were analyzed via a Waters Acquity UPLC (Waters Corporation, Milford, MA) with a gradient of 0.1% formic acid in water and 0.1% formic acid in acetonitrile on a BEH C8 2.1×50 1.7 mcm column (Waters Corporation). The internal standards were tolbutamide and warfarin. Samples were quantified by positive LC-ESI-MS/MS-Multiple Reaction Monitoring (MRM) using an API4000 (AB SCIEX, Framingham, MA) at unit/unit resolution with the heater set at 500C, spray voltage at 5000 eV, and CAD gas at 4 and data gathered via Analyst Software (AB SCIEX) and a proprietary Excel program.

Blednov Y.A., Benavidez J.M., Black M., Ferguson L.B., Schoenhard G.L., Goate A.M., Edenberg H.J., Wetherill L., Hesselbrock V., Foroud T, & Harris R.A. (2014). Peroxisome proliferator-activated receptors α and γ are linked with alcohol consumption in mice and withdrawal and dependence in humans. Alcoholism, clinical and experimental research, 39(1), 136-145.

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Measuring Freshwater Cyanotoxins and Nutrients

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Water temperature and light intensity data were measured every 30 min (HOBO Pendant^® UA-002-64, Onset, MA, USA). Daily rainfall and river discharge data were provided by the Tasman District Council, New Zealand. Analysis of nitrite-N, nitrate-N, ammoniacal-N and DRP were carried out using a flow injection analyzer, following a modified APHA 4500 protocol [27 ]. Anatoxin-a, homoanatoxin-a, dihydroanatoxin-a, and dihydrohomoanatoxin-a were measured using LC-MS/MS [15 ]. Briefly, biofilm samples were lyophilized and 1 mL of Milli-Q water with 0.1% formic acid. Compounds were then separated by liquid chromatography (Waters Acquity UPLC, Waters Corp., MA, USA) on a BEH C18 column (1.7 μm, 1 × 50 mm, Waters Corp., MA, USA) and quantified on a Quattro Premier XE triple quadrupole mass spectrometer (Waters-Micromass, Manchester, UK).

Tee H.S., Waite D., Payne L., Middleditch M., Wood S, & Handley K.M. (2020). Tools for successful proliferation: diverse strategies of nutrient acquisition by a benthic cyanobacterium. The ISME Journal, 14(8), 2164-2178.

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