Fluorescein isothiocyanate fitc conjugated annexin 5 apoptosis detection kit

Dulbecco's modified Eagle's medium (DMEM), trypsin-EDTA, and penicillin-streptomycin were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Fetal bovine serum (FBS), TRIzol^® Reagent, JC-1, and Caspase-3 and -9 Colorimetric Protease Assay kits were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). SuperScript II reverse transcriptase was purchased from Thermo Fisher Scientific, Inc. The B-cell lymphoma 2 (Bcl-2; #2872; 1:1,000), Bcl-2-associated X protein (Bax; #2772; 1:1,000) and β-actin (#4967; 1:1,000) primary antibodies, and the horseradish peroxidase (HRP)-conjugated secondary antibodies (#7075; 1:2,000) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The fluorescein isothiocyanate (FITC)-conjugated Annexin V Apoptosis Detection kit was purchased from BD Biosciences (San Jose, CA, USA). All the other reagents, unless otherwise stated, were obtained from Sigma-Aldrich (St. Louis, MO, USA).

α-mangostin (chemical structure shown in Fig. 1) was purchased from Sigma-Aldrich (St. Louis, MO, USA), dissolved in dimethyl sulfoxide (DMSO) and stored at −20°C. RPMI-1640 medium, penicillin-streptomycin, trypsin-EDTA and fetal bovine serum (FBS) were purchased from HyClone Laboratories, Inc. (Logan, UT, USA). 3-(4,5-Dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and DMSO were obtained from Sigma-Aldrich. Cell lysis buffer and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). The fluorescein isothiocyanate (FITC)-conjugated Annexin V Apoptosis Detection kit was purchased from BD Biosciences (San Diego, CA, USA). Anti-β-actin (#4967), anti-Bax (#2772), anti-Bcl-2 (#2876), anti-caspase-3 (#9662), anti-cleaved caspase-3 (#9661), anti-caspase-9 (#9502), anti-poly(ADP-ribose) polymerase (PARP; #9542), anti-ERK1/2 (#9102), anti-phosphorylated (p)-ERK1/2 (#4376), anti-p38 (#9212), anti-p-p38 (#4631), anti-c-myc (#9027), anti-Ki-67 (#9027) and goat anti-rabbit horseradish peroxidase (HRP)-conjugated (#7074) antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). The DeadEnd™ fluorometric terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay kit was purchased from Promega Corp. (Madison, WI, USA).