Tissue was dissected and cultured based on previous protocols with modifications for the pupal hindgut (Fox et al., 2010 (link); Prasad et al., 2007 (link)). For colcemid live imaging experiments, pupae were dissected and imaged in media containing 50 μg/ml of colcemid (Sigma, St. Louis, MO) from the initiation of dissection to the first frame was at least 15 min and up to 1 hr. Imaging was performed on a spinning disc confocal (Yokogawa CSU10 scanhead) on an Olympus IX-70 inverted microscope using a 60x/1.3 NA UPlanSApo Silicon oil, 100x/1.4 NA U PlanSApo oil, or a 40x/1.3 NA UPlanFl N Oil objective, a 488 nm and 568 nm Kr-Ar laser lines for excitation and an Andor Ixon3 897 512 EMCCD camera. The system was controlled by MetaMorph 7.7.
Csu10 scanhead
Manufactured by Olympus
The CSU10 scanhead is a laboratory equipment component designed for microscopy applications. It features a high-speed confocal scanning unit that enables real-time imaging of samples. The scanhead provides a core function of acquiring high-resolution, low-noise images through its specialized optical and scanning mechanisms.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using csu10 scanhead
1
Pupal Hindgut Live Imaging Protocol
2
Live-Imaging of Cellular Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Live-imaging preparations were prepared as previously described (Prasad et al., 2007 (link); Fox et al., 2010 (link)). Imaging was performed on a spinning disk confocal (Yokogawa CSU10 scanhead) on an Olympus IX-70 inverted microscope using a 60×/1.3 NA UPlanSApo Silicon oil, 488 and 568 nm Kr-Ar laser lines for excitation, and an Andor Ixon3 897 512 electron-multiplying charge-coupled device camera. The system was controlled by MetaMorph 7.7. Images were analyzed in ImageJ (Schneider et al., 2012 (link)).
3
Live Cell Imaging with Spinning Disk Confocal
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissues were prepared for live imaging as described in previous studies (Fox et al., 2010; Schoenfelder et al., 2014) Images were acquired on spinning disk confocal (Yokogawa CSU10 scanhead) on an Olympus IX-70 inverted microscope using a 60×/1.3 NA UPlanSApo Silicon oil, 488 and 568 nm Kr-Ar laser lines for excitation, and an Andor Ixon3 897 512 electron-multiplying charge-coupled device camera. The system was controlled by MetaMorph 7.7
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!