Agilent 7683 automatic liquid sampler

The dentification of the chemical constituents of the essential oil was carried out using an Agilent gas chromatograph (GC) (6890N series, Agilent Technologies, Santa Clara, CA, USA). For the quantitative analysis gas chromatograph was equipped with a flame ionization detector (FID) and for qualitative analysis gas chromatograph was coupled to a mass spectrometer (quadrupole) detector (MS) (model Agilent 5973 inert series, Agilent Technologies, Santa Clara, CA, USA). The GC-FID and GC-MS analyses were performed according to the procedure described by Valarezo et al. [35 (link)]. The injection of the samples was carried out by an automatic injector (Agilent 7683 automatic liquid sampler, Agilent Technologies, Santa Clara, CA, USA) in split mode. Chromatographic runs were performed using a nonpolar and a polar column. The nonpolar was an Agilent J&W DB-5ms Ultra Inert GC column with stationary phase 5%-phenyl-methylpolyxilosane and the polar was an Agilent J&W HP-INNOWax GC column with stationary phase polyeth-ylene glycol. Both columns with a length of 30 m, an outer diameter of 0.25 mm and a stationary phase thickness of 0.25 µm. Identification of the EO compounds was based on a comparison of relative retention indices (RIs) and mass spectra data with those of the published literature [38 ,39 ] according as described by Valarezo et al. [36 (link)].

For quantitative analysis, an Agilent gas chromatograph (GC) (model 6890N series, Agilent Technologies, Santa Clara, CA, USA) equipped with a flame ionization detector (FID) was used. The GC-FID analyses were performed using a nonpolar Agilent J&W DB-5 ms Ultra Inert GC column (30 m, 0.25 mm, 0.25 µm) (5%-phenyl-methylpolyxilosane), a polar Agilent J&W HP-INNOWax GC column (30 m, 0.25 mm, 0.25 µm) (polyethylene glycol), and an automatic injector (Agilent 7683 automatic liquid sampler, Agilent Technologies, Santa Clara, CA, USA) in split mode. The samples, 1 µL of solution (1/100, v/v, EO/DCM), were injected with a split ratio of 1:50. Helium was used as a carrier gas at 1 mL/min in constant flow mode and at an average velocity of 25 cm/s. The initial oven temperature was held at 50 °C for 3 min and then it was heated to 230 °C with a ramp of 3 °C/min, and the temperature was maintained for 3 min until the end. The injector and detector temperatures were 250 °C. Quantification was done by external standard method using calibration curves generated by running GC analysis of representative compounds.

For the quantitative analysis, an Agilent gas chromatograph (GC) (model 6890N series, Agilent Technologies, Santa Clara, CA, USA) equipped with a flame ionization detector (FID) was used. The GC-FID analyses were performed using a nonpolar Agilent J&W DB-5ms Ultra Inert GC column (30 m, 0.25 mm, 0.25 µm) (5%-phenyl-methylpolyxilosane) and an automatic injector (Agilent 7683 automatic liquid sampler, Agilent Technologies, Santa Clara, CA, USA) in split mode. The samples, 1 µL of solution (1/100, v/v, EO/DCM), were injected with a split ratio of 1:50. Helium was used as a carrier gas at 1 mL/min in constant flow mode and an average speed of 25 cm/s. The initial oven temperature was maintained at 50 °C for 3 min and then it was heated to 230 °C with a ramp of 3 °C/min, and the temperature was maintained for 3 min until the end. The injector and detector temperatures were 250 °C. Quantification was done by the external standard method using calibration curves generated by running GC analysis of representative compounds.

The GC-MS system was an Agilent 6890N GC/5975C VL MSD system equipped with an Agilent 7683 Automatic Liquid Sampler (Agilent technologies, Inc., Santa Clara, CA, USA). The column was a DB-WAX column (30 m × 0.25 mm, 0.25 µm; Agilent J&W Scientific, Folsom, CA, USA). The inlet temperature of the GC was kept at 240 °C. Helium (≥99.999%) was used as carrier gas with a constant linear velocity of 1.0 mL/min. 1-μL aliquot was injected in splitless mode. The temperature program that was optimized for GC was as follows: the initial oven temperature as 70 °C, held for 1 min; 20 °C /min to 150 °C; 10 °C/min to 190 °C; 3 °C/min to 220 °C; 5 °C/min to 230 °C; 230 °C held for 12 min. The MS conditions were as follows: electron impact mode at ionization energy of 70 eV; ion source temperature at 230 °C; transfer line temperature at 240 °C; full scan mode in m/z range 35–550 with 0.3 s/scan velocity. The solvent delay was 3 min.