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Cd4 t cell isolation kit 2 for mouse

Manufactured by Miltenyi Biotec
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The CD4+ T Cell Isolation Kit II for mouse is a lab equipment product that enables the isolation of CD4+ T cells from mouse cell suspensions. It provides a simple and efficient method to obtain a highly pure population of CD4+ T cells for downstream applications.

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Dynal dynabeads, by Thermo Fisher Scientific (3 mentions)

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Isolation kits (10 citations) CD4 isolation kit II (9 citations) Cell isolation kits (5 citations) Isolation Kit II (3 citations)

4 protocols using cd4 t cell isolation kit 2 for mouse

1

Isolation of Murine CD4+ T Cells

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Single-cell suspensions were prepared from the spleens of normal CBA and BL/6 mice, RBCs were lysed, and CD4+ T cells were purified by negative selection using CD4+ T cell isolation Kit II for mouse (Miltenyi Biotec). CD4+ T cell purity was >95% by flow cytometric analysis.
Ponichtera H.E., Shainheit M.G., Liu B.C., Raychowdhury R., Larkin B.M., Russo J.M., Salantes D.B., Lai C.Q., Parnell L.D., Yun T.J., Cheong C., Bunnell S.C., Hacohen N, & Stadecker M.J. (2014). CD209a Expression on Dendritic Cells is Critical for the Development of Pathogenic Th17 Cell Responses in Murine Schistosomiasis. Journal of immunology (Baltimore, Md. : 1950), 192(10), 4655-4665.
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2

Activation of CD4+ T Cells by BMDCs

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CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions. Purified CD4+ T cells (2×105) were cultured with 105 syngeneic BMDCs and stimulated with 100 live eggs plus 8 × 104 anti-CD3/CD28 coated beads (Gibco Dynal Dynabeads) for 72h.
Suresh Kumar Meena Kumari M., Liu P., Jump K., Morales Y., Miller E.A., Shecter I., Stadecker M.J, & Kalantari P. (2024). NLRP3 and AIM2 inflammasomes exacerbate the pathogenic Th17 cell response to eggs of the helminth Schistosoma mansoni. bioRxiv.
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3

CD4+ T Cell Activation Assay

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CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions. Purified CD4+ T cells (2 × 105) were cultured with 105 syngeneic BMDCs and stimulated with 100 live eggs plus 8 × 104 anti-CD3/CD28-coated beads (Gibco Dynal Dynabeads) for 72 h.
Kalantari P., Shecter I., Hopkins J., Pilotta Gois A., Morales Y., Harandi B.F., Sharma S, & Stadecker M.J. (2023). The balance between gasdermin D and STING signaling shapes the severity of schistosome immunopathology. Proceedings of the National Academy of Sciences of the United States of America, 120(13), e2211047120.
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4

Activation of Mouse CD4+ T Cells

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CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions. Purified CD4+ T cells (2 × 105) were cultured with 105 syngeneic BMDCs and stimulated with 80 live eggs plus 8 × 104 anti-CD3/CD28-coated beads (Gibco Dynal Dynabeads) for 72 hr.
Kalantari P., Morales Y., Miller E.A., Jaramillo L.D., Ponichtera H.E., Wuethrich M.A., Cheong C., Seminario M.C., Russo J.M., Bunnell S.C, & Stadecker M.J. (2018). CD209a Synergizes with Dectin-2 and Mincle to Drive Severe Th17 Cell-Mediated Schistosome Egg-Induced Immunopathology. Cell reports, 22(5), 1288-1300.
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