Fasting glucose, total, and high-density lipoprotein (HDL) cholesterol levels were measured in plasma using Infinity™ Glucose Hexokinase Liquid Stable Reagent, Infinity™ Cholesterol, and HDL-CHOLESTEROL Plus Direct method, respectively (Thermo Fisher Scientific Inc., Waltham, MA, USA). Insulin levels were quantified by enzyme immunoassay kits (Mercodia AB, Uppsala, Sweden). Lipopolysaccharide binding protein (LBP) was measured by mouse LBP ELSA kit (Hycult Biotech Inc., Wayne, NJ, USA). Serum amyloid A (SAA) levels were determined by “PHASE” ™ Murine Serum Amyloid A Assay (Tridelta Development Ltd., Maynooth, Ireland). Cytokine levels were measured in plasma (dilution 1:1) using Meso Scale Discovery V-PLEX proinflammatory panel one kit (#K15048D-1) as described in section 2.8. In this case, the calculated detection limits were the following: IFN-γ (0.00988–909 pg/mL), IL-1β (0.374–1,765 pg/mL), IL-2 (0.194–2,595 pg/mL), IL-4 (0.0266–1,680 pg/mL), IL-5 (0.224–1,027 pg/mL), IL-6 (0.335–5,440 pg/mL), IL-10 (0.103–3,930 pg/mL), IL-12p70 (1.79–31,400 pg/mL), KC/GRO (0.0968–1,960 pg/mL), TNF-α (0.0256–619 pg/mL).
Mouse lbp elsa kit
Manufactured by Hycult Biotech
Sourced in United States
The Mouse LBP ELSA kit is a laboratory assay used to quantitatively measure the levels of lipopolysaccharide-binding protein (LBP) in mouse samples. LBP is a protein involved in the immune response to bacterial endotoxins. The kit provides the necessary reagents and protocols to perform this measurement using an enzyme-linked immunosorbent assay (ELISA) technique.
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2 protocols using mouse lbp elsa kit
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Metabolic and Inflammatory Biomarkers
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Metabolic and Inflammatory Biomarkers
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Fasting glucose, total, and high-density lipoprotein (HDL) cholesterol levels were measured in plasma using Infinity™ Glucose Hexokinase Liquid Stable Reagent, Infinity™ Cholesterol, and HDL-CHOLESTEROL Plus Direct method, respectively (Thermo Fisher Scientific Inc., Waltham, MA, USA). Insulin levels were quantified by enzyme immunoassay kits (Mercodia AB, Uppsala, Sweden). Lipopolysaccharide binding protein (LBP) was measured by mouse LBP ELSA kit (Hycult Biotech Inc., Wayne, NJ, USA). Serum amyloid A (SAA) levels were determined by “PHASE” ™ Murine Serum Amyloid A Assay (Tridelta Development Ltd., Maynooth, Ireland). Cytokine levels were measured in plasma (dilution 1:1) using Meso Scale Discovery V-PLEX proinflammatory panel one kit (#K15048D-1) as described in section 2.8. In this case, the calculated detection limits were the following: IFN-γ (0.00988–909 pg/mL), IL-1β (0.374–1,765 pg/mL), IL-2 (0.194–2,595 pg/mL), IL-4 (0.0266–1,680 pg/mL), IL-5 (0.224–1,027 pg/mL), IL-6 (0.335–5,440 pg/mL), IL-10 (0.103–3,930 pg/mL), IL-12p70 (1.79–31,400 pg/mL), KC/GRO (0.0968–1,960 pg/mL), TNF-α (0.0256–619 pg/mL).
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