Uas reaper

Manufactured by Bloomington Drosophila Stock Center

The UAS-reaper is a genetic construct that can be used to induce apoptosis, or programmed cell death, in Drosophila melanogaster. It contains the UAS (Upstream Activating Sequence) regulatory element, which allows for targeted expression of the reaper gene, a key regulator of the apoptotic pathway in Drosophila. This construct provides a tool for researchers to study the mechanisms of cell death in fruit fly models.

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Lab products found in correlation

Uas dicer2, by Bloomington Drosophila Stock Center (1 mentions) Delta lacz, by Bloomington Drosophila Stock Center (1 mentions) Vglut gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas mgfp, by Bloomington Drosophila Stock Center (1 mentions) Uas tetxlc, by Bloomington Drosophila Stock Center (1 mentions) Tdc2 gal4, by Bloomington Drosophila Stock Center (1 mentions)

3 protocols using uas reaper

Genetic Manipulation of Drosophila Larvae

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The following strains were used: w¹¹⁸ (control), pcol-Gal4;UAS-mCD8-GFP (pcol>GFP) [11 (link)], PG125 dome-gal4 (dome>) [29 (link)], Cg25C-GFP [30 (link)], hhF4fGFP [31 (link)], UAS-col RNAi [20 (link)], UAS-srp RNAi (GD12779 Vienna Drosophila RNAi Center), UAS-Myc and UAS-TCF^DN [20 (link)], UAS-reaper and UAS-Dicer2 (Bloomington Stock Center). Larvae were raised, in uncrowded conditions from eggs collected for 8 or 12 hours, at 27°C except for RNAi interference experiments which were performed at 29°C. UAS-Dicer2 was co-expressed with RNAi constructs. Larvae were developmentally staged at 29°C as follows: early third larval instar (EL3): 66H after egg laying (AEL); mid third larval instar (ML3): 85H AEL, late third larval instar (LL3): 105H AEL.

Oyallon J., Vanzo N., Krzemień J., Morin-Poulard I., Vincent A, & Crozatier M. (2016). Two Independent Functions of Collier/Early B Cell Factor in the Control of Drosophila Blood Cell Homeostasis. PLoS ONE, 11(2), e0148978.

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Fly Genetic Toolkit for Stem Cell Research

Cited 2 times

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Fly stocks used in this study are as follows: w; esg-Gal4, Tub-Gal80^ts, UAS-GFP ; UAS-Flp, Act>CD2>Gal4 (esg^tsF/O) (25 (link)), w; esg-Gal4, Tub-Gal80^ts, UAS-GFP (52 (link)), esg-Gal4, Tub-Gal80^ts, Su(H)GBE-Gal80, 2xYFP (31 (link)), Delta-Gal4 (26 (link)), Delta-LacZ (Bloomington 11651), Gbe+Su(H)-lacZ [Su(H)-LacZ] (53 (link)), Raptor-RNAi (Bloomington 31529), Akt-RNAi (Bloomington 33615), Notch-RNAi (Bloomington 27988), UAS-GFP-E2F1; UAS-mRFP1-NLS-CycB (Fly-FUCCI) (29 (link)), UAS-Reaper (Bloomington 5824). Flies were maintained at 25°C, on medium containing agar 0.6% (w/v), malt 6.5% (w/v), semolina 3.2% (w/v), baker’s yeast 1.8% (w/v), nipagin 2.4%, and propionic acid 0.7%. In experiments using the temperature sensitive Gal80^ts, flies were reared at +18°C and then kept at +29°C to inactivate the Gal80^ts protein allowing the UAS-Gal4–driven transgene expression. The exact timings of the dietary treatment and temperature switch in each experiment are provided in the figure panels.

Mattila J., Viitanen A., Fabris G., Strutynska T., Korzelius J, & Hietakangas V. (2024). Stem cell mTOR signaling directs region-specific cell fate decisions during intestinal nutrient adaptation. Science Advances, 10(6), eadi2671.

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Drosophila Neuronal Lineage Tracing

Cited 1 time

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The following strains were used: Janelia Gal4 lines 24G08 Gal4 (Bloomington stock 49316) and 31B08 Gal4 (Bloomington stock 49351), Tdc2 Gal4 (Bloomington stock 9313), VGlut Gal4 (Bloomington stock 26160), VGlut MiMIC RMCE line with EGFP reporter (and GFDTF tagged) (Nagarkar-Jaiswal et al., 2015 (link)) (Bloomington stock 59411), UAS mGFP (Bloomington stocks 35839), UAS reaper (Bloomington stock 5824), UAS TeTxLC (Bloomington stock 28997). All crosses were raised at 25°C. The white Canton S (w^CS) strain (outcrossed to CS, from Jean-Maurice Dura, IGH Montpellier, France) was used as wild type control and was crossed with the different Gal4 lines for control experiments.

Pons M., Soulard C., Soustelle L., Parmentier M.L., Grau Y, & Layalle S. (2017). A New Behavioral Test and Associated Genetic Tools Highlight the Function of Ventral Abdominal Muscles in Adult Drosophila. Frontiers in Cellular Neuroscience, 11, 371.

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