Trypsin edta 0.05

All chemicals were purchased from Sigma-Aldrich unless indicated otherwise. HS-PEG-OMe and HS-PEG-COOH MW 5000 Da were provided by JenKem Technology (Plano, TX, USA). Human hippocampal astrocytes, human brain-vascular pericytes, poly-L-lysine, trypsin/EDTA 0.05%, and astrocyte and pericyte media were supplied by Sciencell (Carlsbad, CA, USA). The human brain endothelial cell line (hCMEC/D3) and EndoGRO™ MV cell medium kit were purchased from Merck-Millipore (Billerica, MA, USA), and the MTS reagent from PROMEGA. Aβ Amyloids 1–42 were purchased from Genecust. Alexa 647 hydrazide (1 mg) was obtained from Thermo Fischer Scientific (Waltham, MA, USA). The SYLGARD™ 184 Silicone Elastomer Kit for polydimethyl siloxane (PDMS) was purchased from Ellsworth (Pierce County, WI, USA). Deionized Milli-Q water of 18 MW x cm was obtained using Milli-Q^® purification equipment from Merck Millipore (Darmstadt, Germany), using previously deionized conventional water (acid and basic columns).

All chemicals were purchased from Sigma-Aldrich unless indicated otherwise. HS-PEG-OMe and HS-PEG-COOH MW 5000 Da were provided by JenKem Technology (Plano, TX, USA). Human hippocampal astrocytes, human brain-vascular pericytes, poly-L-lysine, trypsin/EDTA 0.05%, and astrocyte and pericyte media were supplied by Sciencell (Carlsbad, CA, USA). The human brain endothelial cell line (hCMEC/D3) and EndoGRO™ MV cell medium kit were purchased from Merck-Millipore (Billerica, MA, USA), and the MTS reagent from PROMEGA. Aβ Amyloids 1–42 were purchased from Genecust. Alexa 647 hydrazide (1 mg) was obtained from Thermo Fischer Scientific (Waltham, MA, USA). The SYLGARD™ 184 Silicone Elastomer Kit for polydimethyl siloxane (PDMS) was purchased from Ellsworth (Pierce County, WI, USA). Deionized Milli-Q water of 18 MW x cm was obtained using Milli-Q^® purification equipment from Merck Millipore (Darmstadt, Germany), using previously deionized conventional water (acid and basic columns).