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α macroh2a1

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α-macroH2A1.2 is a histone variant protein that is a component of chromatin in eukaryotic cells. It is involved in the regulation of gene expression and chromatin structure.

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Lab products found in correlation

α h2b, by Abcam (4 mentions) α gapdh, by Santa Cruz Biotechnology (4 mentions) Normal mouse igg, by Merck Group (3 mentions) α h2ax, by Abcam (3 mentions) α brca1, by Santa Cruz Biotechnology (3 mentions) α pcna, by Santa Cruz Biotechnology (2 mentions) Goat anti rabbit igg h l hrp, by Thermo Fisher Scientific (2 mentions) Goat anti mouse igg h l hrp, by Thermo Fisher Scientific (2 mentions) α brdu, by BD (2 mentions) α γh2ax, by Merck Group (2 mentions) α phospho s139 h2ax, by Merck Group (2 mentions) α h2a, by Abcam (2 mentions) Img 124a, by Merck Group (2 mentions) α phospho s139 h2ax s139, by Merck Group (2 mentions) α atrx, by Cell Signaling Technology (2 mentions) Img 124a, by Novus Biologicals (2 mentions) Alexa fluor 488 or 647, by Thermo Fisher Scientific (2 mentions) α γ h2ax, by Abcam (1 mentions) α flag m2, by Merck Group (1 mentions) α phospho h2ax, by Merck Group (1 mentions)

4 protocols using α macroh2a1

1

Chromatin Immunoprecipitation (ChIP) Assay

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The following Abs were used for ChIP: α-macroH2A1.2 (Millipore MABE61), α-macroH2A1 (Millipore 07–219), α-phospho-H2AX (Millipore 05–636), α-H2AX (Abcam ab20669), α-H2AZ (Abcam ab4174), α-H2B (Abcam ab52484), normal mouse IgG (Millipore 12–371). Antibodies for IP were α-Flag M2 (Sigma F1804) and α-BRCA1 (Santa Cruz sc-6954). Primary antibodies for western blotting were: α-macroH2A1.2 (Millipore MABE61), α-phospho-H2AX (S139) (Millipore 05–636), α-BRCA1 (Santa Cruz sc-6954), α-PCNA (Santa Cruz sc-56), α-GAPDH (Santa Cruz sc-32233), α-SPT16 (Santa Cruz sc-28734), α-EZH2 (CST 5246s), α-SSRP1 (CST 134215), α-phospho-ATM (CST 4526s), α-phospho-CHK2 (CST 2661s), α-phospho-RPA S4/8 (Bethyl A300-245A), α-RPA2 (Millipore NA19L), α-H2AX (Abcam ab20669), α-H2AZ (Abcam ab4174), α-H2A (CST 12349s, Abcam ab15653). Secondary antibodies were goat anti-mouse IgG-HRP (Santa Cruz sc-2005, Invitrogen 31430) and goat anti-rabbit IgG-HRP (Santa Cruz sc-2004, Invitrogen 31460, CST 7074). Primary antibodies for IF were: α-γ-H2AX (Abcam ab11174, Milipore 05–636), α-BRCA1 (Santa Cruz, sc-6954), α-macroH2A1.2 (Millipore, MABE61), α-pRPA S33 (Bethyl, A300-246A), α-FANCD2 (Novus NB100-182). Secondary antibodies were from Life Technologies (goat-α-mouse or goat-α-rabbit conjugated to Alexa Fluor 488, 568, or 647).
Kim J., Sturgill D., Sebastian R., Khurana S., Tran A.D., Edwards G.B., Kruswick A., Burkett S., Hosogane E.K., Hannon W.W., Weyemi U., Bonner W.M., Luger K, & Oberdoerffer P. (2017). Replication stress shapes a protective chromatin environment across fragile genomic regions. Molecular cell, 69(1), 36-47.e7.
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2

ChIP-Seq and Western Blot Antibodies

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The following antibodies were used for ChIP: α-macroH2A1.2 (Millipore MABE61), α-phospho-S139-H2AX (Millipore 05–636), α-H2B (Abcam ab52484), α-H2A (Abcam ab18255), TRF2 (Novus Biologicals IMG-124A) and normal mouse IgG (Millipore 12–371). Primary antibodies for western blotting were: α-macroH2A1.2 (Millipore MABE61), α-phospho-S139-H2AX (S139) (Millipore 05–636), α-BRCA1 (Santa Cruz sc-6954), α-ATRX (Cell signaling 14820s), α-GAPDH (Santa Cruz sc-32233), α-H2AX (Abcam ab20669). Secondary antibodies were goat anti-mouse IgG (H+L)-HRP (Invitrogen 31430) and goat anti-rabbit IgG (H+L)-HRP (Invitrogen 31460). Primary antibodies for IF were α-BRCA1 (Santa Cruz sc-6954), α-γ-H2AX (Millipore 05–636, Abcam ab11174), α-BrdU (BD Biosciences 555627), and TRF2 (Novus Biologicals IMG-124A), secondary antibodies were goat-anti-mouse or goat-anti-rabbit IgG (H+L) coupled to Alexa Fluor 488 or 647 (Life Technologies).
Kim J., Sun C., Tran A.D., Chin P.J., Ruiz P.D., Wang K., Gibbons R.J., Gamble M.J., Liu Y, & Oberdoerffer P. (2019). The macroH2A1.2 histone variant links ATRX loss to alternative telomere lengthening.. Nature structural & molecular biology, 26(3), 213-219.
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3

Western Blot and Immunofluorescence Antibodies

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The following antibodies were used for western blot: α-macroH2A1.2 (Millipore, MABE61), α-macroH2A1.1 (CST, 12455), α-Lig3 (GeneTex, GTX103172), α-FLAG (Agilent, 200472), α-PCNA (Santa Cruz, sc-56), α-H2B (Abcam, ab1790), α-GAPDH (Santa Cruz, sc-32233), α-PARP1 (CST, 9542S), α-H3 (CST, 9715). Secondary antibodies were goat anti-mouse IgG-HRP (Santa Cruz, sc-2005) and goat anti-rabbit IgG-HRP (Santa Cruz, sc-2004). Primary antibodies for IF were: α-macroH2A1.1 (CST, 12455), α-macroH2A1.2 (Millipore, MABE61), α-centromere (Antibodies incorporated, 15-234-0001), α-PAR (Trevigen, 4335-MC-100), H3K27me3 (Abcam, ab6002), phosphor RPA32 (Ser4 / Ser8) (Bethyl, A300–245A). Secondary antibodies were from Life Technologies (goat-α-mouse, goat-α-rabbit or goat-α-human conjugated to Alexa Fluor 488 or 568).
Sebastian R., Hosogane E.K., Sun E.G., Tran A.D., Reinhold W.C., Burkett S., Sturgill D.M., Gudla P.R., Pommier Y., Aladjem M.I, & Oberdoerffer P. (2020). Epigenetic regulation of DNA repair pathway choice by macroH2A1 splice variants ensures genome stability. Molecular cell, 79(5), 836-845.e7.
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4

ChIP-Seq and Western Blot Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for ChIP: α-macroH2A1.2 (Millipore MABE61), α-phospho-S139-H2AX (Millipore 05–636), α-H2B (Abcam ab52484), α-H2A (Abcam ab18255), TRF2 (Novus Biologicals IMG-124A) and normal mouse IgG (Millipore 12–371). Primary antibodies for western blotting were: α-macroH2A1.2 (Millipore MABE61), α-phospho-S139-H2AX (S139) (Millipore 05–636), α-BRCA1 (Santa Cruz sc-6954), α-ATRX (Cell signaling 14820s), α-GAPDH (Santa Cruz sc-32233), α-H2AX (Abcam ab20669). Secondary antibodies were goat anti-mouse IgG (H+L)-HRP (Invitrogen 31430) and goat anti-rabbit IgG (H+L)-HRP (Invitrogen 31460). Primary antibodies for IF were α-BRCA1 (Santa Cruz sc-6954), α-γ-H2AX (Millipore 05–636, Abcam ab11174), α-BrdU (BD Biosciences 555627), and TRF2 (Novus Biologicals IMG-124A), secondary antibodies were goat-anti-mouse or goat-anti-rabbit IgG (H+L) coupled to Alexa Fluor 488 or 647 (Life Technologies).
Kim J., Sun C., Tran A.D., Chin P.J., Ruiz P.D., Wang K., Gibbons R.J., Gamble M.J., Liu Y, & Oberdoerffer P. (2019). The macroH2A1.2 histone variant links ATRX loss to alternative telomere lengthening.. Nature structural & molecular biology, 26(3), 213-219.
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