Stearoylcarnitine

Fenofibrate and bezafibrate was obtained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). GW6471, ANIT, formic acid, chlorpropamide, lauroylcarnitine (12:0-carnitine), myristoylcarnitine (14:0-carnitine), palmitoylcarnitine (16:0-carnitine), stearoylcarnitine (18:0-carnitine), CA, UDCA, HDCA, CDCA, DCA, TCA, GCA, THDCA, TCDCA, TDCA, TLCA, glycochendeoxycholic acid (GCDCA), glycodeoxycholic acid (GDCA), dehydrocholic acid (DHCA), and glycolithocholic acid (GLCA) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Hyocholic acid (HCA), TβMCA, and ωMCA were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). TUDCA and LCA was purchased from Medchemexpress (Monmouth Junction, NJ, USA). TαMCA and βMCA were purchased from Steraloids (Newport, RI). All solvents and organic reagents were of the highest grade commercially available.

Gradient grade water and LC-MS grade methanol for liquid chromatography plus the non LC-MS grade rinsing solvents 2-propanol and acetonitrile as well as formic acid (puriss.) and ammonia fluoride (puriss.) were purchased from Carl Roth (Carl Roth, Rothenfels, Germany). A collection of all l-amino acids and carnitine, acetylcarnitine, propionylcarnitine, butyrylcarnitine, valerylcarnitine, hexanoylcarnitine, octanoylcarnitine, decanoylcarnitine, lauroylcarnitine, myristoylcarnitine, palmitoylcarnitine and stearoylcarnitine, were purchased from Sigma-Aldrich (St. Louis, MO, USA).
The deuterated internal standards (IS) Cortisol ²H₄, Progesterone ²H₉, 17-Hydroxyprogesterone ²H₈, 11-Deoxycortisol ²H₅, 21-Deoxycortisol ²H₈, Androstenedione ¹³C₃, Corticosterone ²H₄, 11-Deoxycorticosterone ¹³C₃ were also products of Sigma-Aldrich (St. Louis, MO, USA). The deuterated amino acids and acylcarnitines were obtained from the MassChrom^® Amino Acids and Acylcarnitines from Dried Blood kit from Chromsystems (Munich, Germany). Dried blood spot cards (TFN filter paper) were provided by CAMAG (Muttenz, Switzerland). Fresh whole blood was obtained from the local blood donation center (Basel, Switzerland).