Icrt14

Manufactured by MedChemExpress

Sourced in United States

ICRT14 is a laboratory instrument used for cell-based assays. It functions as an integrated cell reporter system that enables real-time monitoring and quantification of cellular responses. The core function of ICRT14 is to provide a platform for researchers to study cellular processes and evaluate the effects of various treatments or stimuli on live cells.

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Lab products found in correlation

4 protocols using icrt14

NDRG3 Regulation by iCRT14

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Inhibitor of β-catenin-responsive transcription 14 is abbreviated as iCRT14 (MedChemExpress). K/G cells were transfected, and the groups were NDRG3+DMSO (Solarbio) and NDRG3+iCRT14. The concentration of iCRT14 was 1 mM (dissolved in DMSO), added iCRT14 after transfection 24 hours, and the final concentration was 25 µM. The experiment was performed after another 24 h.

Wang X., Rong S., Sun Y., Yin B., Yang X., Lu X., Sun H., Yan Y., Sun G., Liang Y., Wang P., Xie S, & Li Y. (2023). NDRG3 regulates imatinib resistance by promoting β‑catenin accumulation in the nucleus in chronic myelogenous leukemia. Oncology Reports, 50(2), 152.

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Investigating JNK/c-Jun Signaling Pathway

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The following chemical reagents were used in this study: iCRT14 (MedChemExpress, cat# HY16665), sp600125 (Cell Signaling Technology, cat#8177). The following antibodies were used in this study: phospho(p)-c-Jun (Ser73) rabbit monoclonal antibody (Cell Signaling Technology, cat# 3270), c-Jun rabbit mAb (Cell Signaling Technology, cat# 9165), p-JNK (Thr183/Tyr185) rabbit mAb (Cell Signaling Technology, cat# 9251), JNK rabbit polyclonal antibody (pAb) (Cell Signaling Technology, cat# 9252). p-β-catenin (Ser552) rabbit mAb (Cell Signaling Technology, cat# 9566). β-catenin (Ser552) rabbit mAb (Abcam, cat# ab32572), mouse control IgG (ABclonal, cat# AC011), rabbit control IgG (ABclonal, cat# AC005), laminA/C mouse mAb (Santa Cruz Biotechnology, cat# sc-376248), β-Tubulin rabbit pAb (Abclonal, cat# AC015), GAPDH mouse mAb (Cell Signaling Technology, cat# 2118), β-actin rabbit mAb (Cell Signaling Technology, cat# 4970), Alexa Fluor 488®-conjugated goat anti-rabbit IgG (H + L) (Invitrogen, cat# A-11008), HRP- (horseradish peroxidase-) conjugated goat anti-mouse IgG (Cell Signaling Technology, cat# 7076), and goat anti-rabbit IgG (Cell Signaling Technology, cat# 7074). Goat anti-BoHV-1 serum was purchased from VMDR Inc (cat# 20PAB-IBR).

Chang L., Yuan W, & Zhu L. (2020). β-cantenin is potentially involved in the regulation of c-Jun signaling following bovine herpesvirus 1 infection. Veterinary Microbiology, 248, 108804.

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Molecular Mechanisms of HMGB1-RAGE Signaling

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iCRT14 (MedChemExpress, cat# HY16665), Glycyrrhizin acid (MedChemExpress, cat# HY-N0184). HMGB1 rabbit monoclonal antibody (mAb) (ABclonal, cat# A19529), RAGE rabbit polyclonal antibody (pAb) (ABclonal, cat# A1395), β-catenin rabbit mAb (Abcam, cat# ab32572), laminA/C mouse mAb (Santa Cruz Biotechnology, cat# sc-376,248), Cox IV rabbit pAb (Cell Signaling Technology, cat# 4844), β-Tubulin rabbit pAb (Abclonal, cat# AC015), GAPDH mouse mAb (Cell Signaling Technology, cat# 2118), BoHV-1 gC mouse mAb (VMDR Inc, cat# F2), BoHV-1 gD mouse mAb (VMDR Inc, cat# 1B8-F11), Alexa Fluor 488®-conjugated goat anti-rabbit IgG (H + L) (Invitrogen, cat# A-11,008), HRP- (horseradish peroxidase-) conjugated goat anti-mouse IgG (Cell Signaling Technology, cat# 7076), and goat anti-rabbit IgG (Cell Signaling Technology, cat# 7074).

Fan W., Yuan W., Ding X, & Zhu L. (2021). β-catenin has potential effects on the expression, subcellular localization, and release of high mobility group box 1 during bovine herpesvirus 1 productive infection in MDBK cell culture. Virulence, 12(1), 1345-1361.

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Wnt3a Signaling Pathway Modulation

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Rabbit anti-Wnt3a and anti-Flag tag antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA (2721s, and 2368s). Rabbit anti-GAPDH and goat anti-rabbit IgG antibodies were purchased from GeneTex (GTX100118 and GTX213110-01). Mouse anti-β-catenin was procured from BD Biosciences (BD610154). Rabbit anti-HA tag antibody was purchased from Millipore (04-902). Normal rabbit IgG was purchased from SantaCruz (sc-2027). Lipofectamine™ 2000 Transfection Reagent (Invitrogen, Carlsbad, CA, USA) and TransIT^®-LT1 Transfection Reagent (Mirus Bio, Madison, WI, USA) were used for plasmid transfection of H1650 and HEK293T cells, respectively. iCRT-14 and LGK-974 were purchased from MedChemExpress, Monmouth Junction, NJ, USA. The control and Wnt3a-conditioned media (CM) were prepared as previously described [35 (link)].

Melano I., Chen H.J., Ngwira L., Hsu P.H., Kuo L.L., Noriega L, & Su W.C. (2023). Wnt3a Facilitates SARS-CoV-2 Pseudovirus Entry into Cells. International Journal of Molecular Sciences, 25(1), 217.

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