The largest database of trusted experimental protocols

22 protocols using oleic acid

1

Synthesis of Metal Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Iron (III) chloride hexahydrate (FeCl3·6H2O, ACS), iron(III) acetylacetonate [Fe(III)(acac)3, 98%], copper (II) chloride dihydrate (CuCl2·2H2O, 99.99%), copper(II) acetylacetonate [Cu(II)(acac)2, 97%], 1-dodecanethiol (DT, 98.0%), sodium diethyldithiocarbamate trihydrate (DDTC, 99.0%), thiourea (CH4N2S, 99.0%), oleylamine (OAM, 80–90%) and 1-octadecene (ODE, >90.0%) were purchased from Shanghai Aladdin Industrial Corporation, China. Oleic acid (OA, >85.0%) were purchased from Tokyo Chemical Industry Co. Absolute ethanol was purchased from Kelong Chemical Reagent Factory, China.
+ Open protocol
+ Expand
2

Dutasteride Formulation Development

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stearic and oleic acid were purchased from Tokyo Chemical Industry (Oxford, UK). Dutasteride (purity > 98.0%) was obtained from Carbosynth (Compton, UK). Ethanol (96% v/v analytical grade) and Sephadex G-50 was obtained from Sigma-Aldrich (Dorset, UK). Phosal® 53 MCT and Lutrol® micro 68 were supplied by Lipoid GmbH (Ludwigshafen, Germany) and BASF Group (Ludwigshafen, Germany), respectively. Water (HPLC grade) was purchased from Fisher Scientific (Loughborough, UK). Deionised water was prepared in-house (PURELAB, ELGA, London, UK).
+ Open protocol
+ Expand
3

Adipogenic Differentiation of CD29+ Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following cell expansion, 1 × 105 CD29+ cells were seeded in a 96-well U-bottom plate (IWAKI, Chiyoda City, Japan) to form spheroids. Subsequently, the plate was centrifuged at 4 × 400× g for 3 min. After 3 days of incubation, spheroids were cultured in a myogenic differentiation medium comprising DMEM with GlutaMax (Life Technologies) supplemented with 5% horse serum (Life Technologies) for 5 days. Next, spheroids were cultured in adipogenic differentiation medium comprising DMEM with GlutaMax (Life Technologies), supplemented with 5% horse serum (Life Technologies), 1 μM IBMX, 1 μM dexamethasone, 1 mM insulin-transferrin-selenium, 50 μM indomethacin, and 500 μM oleic acid (Tokyo Chemical Industry) for 3 days. Subsequently, the medium was replaced with DMEM supplemented with GlutaMax (Life Technologies), 5% horse serum (Life Technologies), 1 μM IBMX, 1 μM dexamethasone, 50 μM indomethacin, and 500 μM oleic acid. Cells were cultured for 2 weeks to facilitate adipogenic differentiation. All chemicals used in the experiment were for research purposes only.
+ Open protocol
+ Expand
4

Formulation and Evaluation of Dexamethasone Eye Drops

Check if the same lab product or an alternative is used in the 5 most similar protocols
NAR was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China), glycerol triacetate was bought from J&K Scientific Ltd. (Beijing, China), oleic acid was obtained from Tokyo Chemical Industries Co., Ltd. (Tokyo, Japan), propylene glycol dicaprylate and castor oil were purchased from Hunan Er-Kang Pharmaceutical Co., Ltd. (Changsha, China), PEG-40 hydrogenated castor oil (Cremophor RH40, CRH40), Cremophor EL35, and Kolliphor HS15 were bought from BASF SE (Ludwigshafen, Germany). Tween 80 was purchased from Sichuan Jinshan Pharmaceutical Co., Ltd. (Guangyuan, China), PEG400 was obtained from Solarbio Life Science (Beijing, China), and dexamethasone (DXMS) sodium phosphate eye drops were obtained from Xinxiang Huaqing Pharmaceutical Co., Ltd. (Xinxiang, China). The other reagents and solvents were analytical grade and high-performance liquid chromatography (HPLC) grade, respectively.
+ Open protocol
+ Expand
5

Lipid Membrane Fluidity Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oxacillin sodium salt (catalog number: sc-224180B, Santa Cruz Biotechnology Inc., Dallas, TX, USA) was dissolved in distilled water. Ricinoleic acid (catalog number: R7257, Sigma-Aldrich Inc., Seoul, Republic of Korea), palmitic acid (catalog number: 129702500, Acros Organics, ThermoFisher Scientific Korea Ltd., Seoul, Republic of Korea), stearic acid (catalog number: S0163, Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), linoleic acid (catalog number: L07949, ThermoFisher Scientific Korea), and oleic acid (catalog number: O0180, Tokyo Chemical Industry) were dissolved in ethanol (catalog number: 000E0243, Samchun Chemicals). Laurdan (6-Dodecanoyl-2-Dimethylaminonaphthalene, catalog number: D250) was purchased from Invitrogen (ThermoFisher Scientific Korea). Phosphate-buffered saline (PBS, catalog number: P5493-1L) was purchased from Sigma-Aldrich Inc.
+ Open protocol
+ Expand
6

Synthesis of Gold Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
DCN was provided by Consolidated Chemical Laboratories (Lahore, Pakistan) as a gift sample. Stearic acid, lauric acid, and oleic acid were sourced from Tokyo Chemical Industry (Tokyo, Japan). Gold chloride, Brij 98 (polyoxyethylene oleyl ether), Tween 80 (polysorbate 80), and soy lecithin were purchased from Acros Organics (Geel, Belgium). Chloroform (high-performance liquid chromatography grade) was purchased from Alfa Aesar (Ward Hill, MA, USA). All materials were of analytical grade.
+ Open protocol
+ Expand
7

Fatty Acid and Surfactant Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lauric acid (C12) and oleic acid (C18:1) were purchased from Tokyo Chemical Industry Co, Ltd (Tokyo, Japan). Caprylic acid (C8), capric acid (C10), linoleic acid (C18:2), SLS, potassium hydroxide (KOH), and Hank's balanced salt solution (HBSS) (+) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). SLES was purchased from the NOF Corporation (Tokyo, Japan).
+ Open protocol
+ Expand
8

Transdermal Delivery System Development

Check if the same lab product or an alternative is used in the 5 most similar protocols
CoQ10, isopropyl myristate (IPM), and oleic acid (OA) were purchased from Tokyo Chemical Industry (TCI, Tokyo, Japan). Labrafil M1944, Labrasol, and Transcutol® HP were provided by Gattefosse (Gattefosse, France). Cottonseed oil was obtained from Daejung Chemicals (Daejung, Korea). Tween 80, propylene glycol (PG), and polyethylene glycol (PEG) 400 were procured from the Duksan Corporation (Duksan, Korea). Cremophor EL® (Kolliphor) was purchased from Sigma–Aldrich (St. Louis, MO, USA). Ethyl alcohol and isopropyl alcohol were of HPLC grade (Duksan, Korea). For the in vitro cell experiments, fetal bovine serum (Gibco®, Grand Island, NY, USA), bovine calf serum (Gibco®, Penrose, AKL, NZ), penicillin–streptomycin (Corning®, Manassas, VA, USA), 0.05% trypsin 0.53 mM EDTA (Corning®, Manassas, VA, USA), and Dulbecco’s modified Eagle’s medium (Corning®, Manassas, VA, USA) were used. For the cell proliferation test, a cell counting kit (CCK-8) was used (Dojindo Molecular Technologies, Inc., Kumamoto, Japan). All other purchased reagents were of analytical grade.
+ Open protocol
+ Expand
9

Synthesis of Pd-Pb Core-Shell Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents and solvents were commercially available. Sodium tetrachloropalladate(II) (Na2PdCl4, 98%), polyvinylpyrrolidone (PVP, Mw ≈ 55,000), ascorbic acid (AA, 99 + %), potassium bromide (KBr, ≥ 99%), oleylamine (OAm, 80–90%), 1-octadecene (ODE, 90%), indium(III) chloride (InCl3, 98%), indium powder (In, >99.99%), eicosane (99%), ammonium hydrogen solution (NH3aq, 28–30%), tetraethyl orthosilicate (TEOS, 98%) and polyoxyethylene nonylphenylether (IGEPAL@CO-520, Mw = 441) were purchased from Sigma-Aldrich. Iron pentacarbonyl (Fe(CO)5, 95%) was purchased from Kanto Chemical Corporation. Oleic acid (OA, > 85%) was purchased from Tokyo Chemical Industry. Lead (II) acetate trihydrate (Pb(OAc)2·3H2O), ethanol, acetone, n-hexane, chloroform, and cyclohexane were purchased from Wako Pure Chemical Industries. Boron nitride (BN, > 99.5%) powder was purchased from Nacalai Tesque. Chemical reagents were used as received without further purification except for the synthetic process of Pd–Pb@FeOy NPs.
+ Open protocol
+ Expand
10

Lipid Analysis of Human Skin Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Linoleic acid (1st Grade, Fujifilm Wako Pure Chemical Corporation, Osaka, Japan), squalene (Special Grade, Fujifilm Wako Pure Chemical Corporation, Osaka, Japan), and oleic acid (O0180, Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) were selected as lipid samples. Human skin tissue derived from the abdomen of a 42-year-old Caucasian female was purchased from Biopredic International (Rennes, France) via KAC (Kyoto, Japan).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!