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2 protocols using tetrabutylammonium borohydride

1

Synthesis of Organic Compounds

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Toluene (99.8%), methyl alcohol (99.9%), and nitric acid (68.0–70.0%) were purchased from Samchun. Tetrahydrofuran (THF) was obtained from Duksan. Hydrochloric acid (36.5–38.0%) was ordered from J.T.Baker. Silk fibroin (5% solution) was purchased from Advanced BioMatrix. Sodium nitrite, 6-bromo-1-hexene, chloroplatinic acid hexahydrate (H2PtCl6 ∙ 6H2O), decanoic acid, hydrazine monohydrate, silver acetate, dodecylamine (DDA), lead(II) bromide, methylammonium bromide (MABr), 4-methoxyaniline, sodium hydride, and tetrabutylammonium borohydride (TBAB) were obtained from Sigma-Aldrich. AZ-5214E was used as photoresist (PR). Sodium hydroxide, phenol, and petroleum ether were ordered from Daejung. N,N-dimethylformamide (DMF) was purchased from Honeywell. Silicone elastomer base and silicone elastomer curing agent (Sylgard 184) were obtained from Dow Corning.
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2

Buffers for Immunoassay Development

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All buffers were prepared in Milli-Q water and stored in amber glass bottles at room temperature (RT, 22 ± 1 °C) unless stated otherwise. The pH values were adjusted using 6 M hydrochloric acid (Merck) or 5 M sodium hydroxide solution (J.T.Baker, Phillipsburg, NJ, USA).

Phosphate-buffered saline (PBS), pH 7.6: 10 mM sodium phosphate monobasic dihydrate (Sigma-Aldrich), 70 mM sodium phosphate dibasic dihydrate (Sigma-Aldrich), 145 mM sodium chloride (Sigma-Aldrich).

Washing buffer, pH 7.6: 0.75 mM potassium phosphate monobasic (Sigma-Aldrich), 6.25 mM potassium phosphate dibasic (Sigma-Aldrich), 0.025 mM potassium sorbate (Sigma-Aldrich), 0.05% Tween 20 (Serva, Heidelberg, Germany).

Assay buffer (Tris–EDTA), pH 7.6, storage at 4 °C: 125 mM tris(hydroxymethyl)-aminomethane (Tris, Merck), 187.5 mM sodium chloride, 13.375 mM ethylenediaminetetraacetic acid disodium salt dihydrate (Na2EDTA·2H2O, Sigma-Aldrich).

Citrate buffer, pH 4.0, storage at 4 °C: 220 mM sodium citrate monobasic (Sigma-Aldrich).

TMB stock solution in dry N,N-dimethylacetamide (DMA, Sigma-Aldrich), storage under argon at 4 °C: 8 mM tetrabutylammonium borohydride (Sigma-Aldrich), 40 mM 3,3’,5,5’-tetramethylbenzidine (TMB, Serva).

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