Cdk2 mouse monoclonal antibody

Protein levels of PCBP2 and CDK2 were detected using western blotting, which was performed essentially as described previously 16, 17. PCBP2 rabbit polyclonal antibody, CDK2 mouse monoclonal antibody (both dilution 1 : 1000; Proteintech Group, Inc.) and GAPDH rabbit polyclonal antibody (dilution 1 : 10 000; Proteintech Group, Inc.) were used. GAPDH was examined as a control.

Immunohistochemistry was carried out to analyze the protein levels of PCBP2 and CDK2 in paraffin sections of human gastric cancer tissues or adjacent normal gastric tissues. As described previously, a two‐step histostaining method (Maixin, Fuzhou, China) and PCBP2 Rabbit Polyclonal antibody (1 : 200; Proteintech Group, Inc., Chicago, IL, USA)/CDK2 Mouse Monoclonal antibody (dilution 1 : 100; Proteintech Group, Inc.) were used in the immunohistochemical study 16, 17. The stained sections were evaluated using an Olympus microscope (Olympus America, Inc., Melville, NY, USA). At least 10% of cells positively stained in the sections were designated as PCBP2‐positive and less than 10% cells positively stained in the sections were designated as PCBP2‐negative. Regarding the semiquantitative analysis of the staining intensity distribution of PCBP2 levels in HGC: 30–60% cells positively stained in the sections were designated as PCBP2‐moderate, less than 30% cells positively stained in the sections were designated as PCBP2‐weak and more than 60% cells positively stained in the sections were designated as PCBP2‐strong. The average staining intensity of gastric cancer tissue samples was 58.1% for PCBP2. For Kaplan–Meier analysis, less than 58.1% cells positively stained in the sections were designated as PCBP2‐low and more than 58.1% cells stained were designated as PCBP2‐high.