Calcium peroxide

Manufactured by Merck Group

Sourced in United States

Calcium peroxide is a chemical compound with the formula CaO2. It is a white, odorless powder that is used as an oxidizing agent in various industrial and laboratory applications.

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Lab products found in correlation

5 protocols using calcium peroxide

Wound Healing Biomaterial Composition

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Sodium percarbonate (SPO) (20–30% H₂O₂), calcium peroxide (CPO) (75% purity), fibrinogen from bovine plasma (type I-S, 65–85% protein; ≥75% of protein was clottable), hyaluronic acid sodium salt (HA) from Streptococcus equi, glycerol, gelatin from porcine skin (gel strength: ~175 g Bloom, type A), thrombin from bovine plasma, aprotinin from bovine lung, and catalase from bovine liver (2000–5000 U/mg) were purchased from Sigma Aldrich (St. Louis, MO, USA).

Ke D., Kengla C., Lee S.J., Yoo J.J., Zhu X, & Murphy S.V. (2022). Release Kinetics and In Vitro Characterization of Sodium Percarbonate and Calcium Peroxide to Oxygenate Bioprinted Tissue Models. International Journal of Molecular Sciences, 23(12), 6842.

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Photocrosslinkable Gelatin Hydrogel

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Gelatin (Type A, 300 bloom from porcine skin), methacrylic anhydride (MA), calcium peroxide (CPO), catalase (obtained from bovine liver, 2000 – 5000 units mg⁻¹ protein), 2-hydroxy-4’-(2-hydroxyethoxy)-2-methyl propiophenone (photoinitiator, PI), phosphate-buffered saline (PBS) and Alizarin red S were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s phosphate buffered saline (DPBS), trypsin-EDTA, and penicillin−streptomycin were purchased from Fisher Scientific. Alpha-modified Eagle’s medium (Gibco 11965–092, Invitrogen Co., USA was supplied by Invitrogen (Grand Island, NY, USA). FBS and precleaned microscope slides were obtained from Fisher Scientific (Waltham, MA, USA). The live/dead viability/cytotoxicity kit was obtained from Invitrogen (Grand Island, NY, USA). All reagents were used without further purification.

Erdem A., Darabi M.A., Nasiri R., Sangabathuni S., Ertas Y.N., Alem H., Hosseini V., Shamloo A., Nasr A.S., Ahadian S., Dokmeci M.R., Khademhosseini A, & Ashammakhi N. (2020). Three Dimensional Bioprinting of Oxygenated Cell-Laden Gelatin Methacryloyl Constructs. Advanced healthcare materials, 9(15), e1901794.

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Suture Coating with PLGA and Calcium Peroxide

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PLGA (Lakeshore Biomaterials, Short Hills, New Jersey, USA) 15 per cent w/v (85 : 15) was dissolved for 3 h in dimethyl sulphoxide (DMSO) (Fisher Scientific, Pittsburgh, Pennsylvania, USA). Calcium peroxide (Sigma‐Aldrich, Burlington, Massachusetts, USA) 10 per cent w/w was added to the PLGA solution. The mixture was stirred for 2 h. Once all components had dissolved, the solution was transferred to a 15‐ml conical tube and stored at −80°C until use.
Vicryl™ 6/0 coated sutures with a P‐1 needle (Ethicon) were wrapped around 1‐cm glass rods and secured with tape. The glass rods were suspended into the oxygenated polymer solution for 5 min. To disperse the CPO particles evenly, samples were vortexed every minute. Sutures were placed on glass trays and allowed to dry overnight. They were then dipped into a 100 per cent ethanol solution to remove any residual DMSO. The samples were air‐dried on plastic trays for a further hour. Sutures were repackaged into the original containers and sterilized using γ radiation (1 mrad, 1 h).
A methylthiazolyldiphenyl‐tetrazolium bromide (Invitrogen™; Fisher Scientific) colorimetric assay, used to evaluate compatibility of human BJ (3T3; American Type Culture Collection (ATCC), Wesel, Germany) fibroblasts with the different suture materials, showed no toxicity (data not shown).

Inglin R.A., Brügger L.E., Candinas D., Harrison B.S, & Eberli D. (2019). Effect of oxygen‐producing suture material on hypoxic colonic anastomoses in an experimental model. BJS Open, 3(6), 872-881.

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Preparation of Polymeric Drug Delivery Systems

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The following chemical reagents were obtained: pectin (from citrus, TCI Europe), polylactic acid (PLA, Goodfellow, granular, 3 mm nominal granule size, Sigma Aldrich), ethyl lactate ((S)-(-)-ethyl lactate for synthesis, Merck KGaA, Sigma Aldrich), ethanol (ACS reagent, ≥99.5%, Sigma Aldrich), indomethacin (analytical grade, ≥99% purity, Sigma Aldrich), diclofenac sodium salt (diclofenac, 10 µg/mL in acetonitrile, 98.0% purity, CHEMOS GmbH & Co. KG), calcium peroxide (75%, −200 mesh, Sigma Aldrich), sodium percarbonate (avail. H₂O₂ 20–30%, Sigma Aldrich). All other chemical reagents were of analytical grade (Sigma Aldrich), and deionized water was used throughout.

Horvat G., Žvab K., Knez Ž, & Novak Z. (2023). Hybrid Polylactic-Acid–Pectin Aerogels: Synthesis, Structural Properties, and Drug Release. Polymers, 15(2), 407.

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Fabrication and Characterization of Gelatin-based Hydrogel

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Porcine skin gelatin, methacrylic anhydride (MAA), and calcium peroxide were obtained from Sigma-Aldrich (St. Louis, MO). Polycaprolactone (PCL) was procured from Fisher Scientific. Dulbecco's phosphate buffered saline (DPBS), Dulbecco's Modified Eagle's Medium (DMEM – low glucose), fetal bovine serum (FBS), trypsin-ethylenediaminetetraacetic acid (EDTA) 0.25%, and penicillin/streptomycin (P/S) were procured from Gibco (Thermo Fisher Scientific, Waltham, MA). Alamar Blue reagent was obtained from Invitrogen (Grand Island, NY). 2-hydroxy-1-[4-(hydroxyethoxy) phenyl]-2-methyl-1propanone (Irgacure 2959) was acquired from BASF Corporation (Florham Park, NJ). Lactate Dehydrogenase (LDH) activity kit was purchased from Thermo Fisher Scientific (Waltham, MA). Caspase Glo 3/7 assay kit was procured from Promega (Madison, WI). NeoFox Oxygen sensing probe was purchased from Ocean Optics Inc (Orlando, FL). These reagents were used in the formulation as received without further purification of the products.

Suvarnapathaki S., Wu X., Zhang T., Nguyen M.A., Goulopoulos A.A., Wu B, & Camci-Unal G. (2021). Oxygen generating scaffolds regenerate critical size bone defects. Bioactive Materials, 13, 64-81.

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