Chitosan oligosaccharide (COS), triethylamine (TEA), N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDAC), N-hydroxy succinimide (NHS), and dimethyl sulfoxide (DMSO) were purchased from Tokyo Chemical Industry (TCI) Co., LTD. (Tokyo, Japan). Chlorin e6 (Ce6) was obtained from Frontier Sci. Co. (Logan, UT, USA). Thioketal dicarboxylic acid (ThdCOOH) was purchased from RuixiBiotech Co. Ltd. (Xi’an, China). Hydrogen peroxide (H2O2), phosphotungstic acid, 4-(aminomethyl) phenylboronic acid pinacol ester hydrochloride (PBAP), 2′,7′-dichlorofluorescin diacetate (DCFH-DA), 3-(4,5-dimethyl2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT), and 2,2,2-tribromoethanol (avertin) were purchased from Sigma Aldrich Chem. Co. (St. Louis, MO, USA). Dialysis membranes with molecular weight cutoffs (MWCO) of 1000 or 2000 Da were purchased from Spectrum Labs., Inc. (Rancho Dominguez, CA, USA). 2,2,2-tribromoethanol (avertin) and tert-amyl alcohol were purchased from Sigma Aldrich Chem. Co. (St. Louis, MO, USA).
5 diphenyl 2h tetrazolium bromide mtt
Manufactured by Merck Group
Sourced in United States
5-diphenyl-2H-tetrazolium bromide (MTT) is a chemical compound used in various laboratory procedures. It functions as a colorimetric assay for assessing cell metabolic activity. The compound undergoes reduction in the presence of metabolically active cells, resulting in the formation of a purple formazan product that can be quantified spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
3 4 5 dimethyl 2 thiazolyl 2, by Merck Group (7 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Bovine serum albumin (bsa), by Solarbio (2 mentions)
Bca protein assay kit, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
Qpcr mastermix plus for sybr green 1 dntp, by Thermo Fisher Scientific (2 mentions)
2 7 dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions)
Avertin 2 2 2 tribromoethanol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Tokyo Chemical Industry (1 mentions)
Chitosan oligosaccharide, by Tokyo Chemical Industry (1 mentions)
N hydroxysuccinimide, by Tokyo Chemical Industry (1 mentions)
Tert amyl alcohol, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Sangon (1 mentions)
Bis acrylamide, by Merck Group (1 mentions)
Anti bax, by Abcam (1 mentions)
Anti caspase 3, by Abcam (1 mentions)
10 protocols using 5 diphenyl 2h tetrazolium bromide mtt
1
Chitosan-based Phototherapeutic Platform
2
Nanocomposite for Tissue Engineering
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PLGA [molecular weight (MW) = 150000, PLA:PGA = 75:25 (mol/mol)] and needle-like HA nanocrystals (HA) of 20−30 nm in diameter and 100−200 nm in length were synthesized by Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (CIAC, China). GO was purchased from Chengdu Organic Chemicals Co. Ltd, China (thickness: 0.55–1.2nm, diameter: 0.5–3μm). Bovine serum albumin (BSA) was obtained from Beijing Solarbio Science & Technology Co., Ltd. 1,1, 1, 3, 3,3-hexafluoroisopropanol (HFIP), 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2- H-tetrazolium bromide (MTT), and BCA protein assay kit were purchased from Sigma-Aldrich (USA). The reagents for cell experiments were purchased from Gibco (USA).
3
Cell Viability and Apoptosis Assay
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Phosphate-buffered saline (PBS, pH = 7.4) was purchased from Sangon Co, Ltd (China), 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT), sodium dodecyl sulfate (SDS), polyacrylamide, bis-acrylamide, and TARMA were purchased from Sigma-Aldrich Co, Ltd (USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin-streptomycin solution were purchased from Invitrogen. Other chemicals were purchased from Sigma-Aldrich Co, Ltd. Anti-Bcl-2, anti-Bax, and anti-Caspase-3 proteins were purchased from Abcam Co. Ltd (Great Britain).
4
Synthetic Curcumin Analogs: Characterization and Evaluation
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RL71, RL100 (3,5-bis(3,4,5-trimethoxybenzylidene)- piperidine-4-one), F36 (3,5-bis(3,4-dimethoxybenzylidene)- piperidine-4-one), LH60 (3, 5- di(3, 4, 5- trimethoxybenzylidene) tetrahydro- 2H- pyran- 4- one), LH40 (3, 4- dihydro- 4, 6- bis(3, 4, 5- trimethoxyphenyl) - 2(1H) - pyrimidinethione) and other synthetic Curcumin analogs were kindly provided by Professor Guang Liang from Wenzhou Medical College, China. These structures were confirmed by comparing MS, 1H NMR and physical data with those reported in the literature [32 (link), 34 (link)]. The purity is higher than 97%. Curcumin (>98% purity), 5-Diphenyl-2H-tetrazolium bromide (MTT) and thapsigargin were purchased from Sigma-Aldrich (St. Louis, MO). ER-tracker Red and Fura-2/AM were purchased from Beyotime (Nanjing).
5
Smart Polymer-Based Drug Delivery
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Acrylic acid (AA) as a pH-responsive monomer, Vinylcaprolactam (VCL) as a temperature-responsive monomer, and triethylene glycol dimethacrylate (TEGDMA) were obtained from Merck Chemical Co. (Darmstadt, Germany). Potassium persulfate (KPS) was obtained from Sigma-Aldrich, Chemical Co. (St. Louis, MO USA). Sodium dodecyl sulfate (SDS) was obtained from Merck (Darmstadt, Germany). Doxorubicin (Dox) was obtained from Ebewe (Ebewe pharma, Austria). The cell culture medium (RPMI-1640), penicillin-streptomycin, and FBS were obtained from Gibco Company, Scotland. MCF-7 and HFF-2 cell lines were purchased from the Pasteur Institute, Iran. Dimethyl sulfoxide (DMSO), trypsin, and 5-diphenyl-2-H-tetrazolium bromide (MTT) were obtained from Sigma-Aldrich Chemical Co., USA.
6
Preparation of Graphene Oxide-Embedded PLGA Scaffolds
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PLGA (molecular weight = 80000, LA/GA = 75/25) was purchased from SinoBiomaterials Co., Ltd. BMP-2 was purchased from UB Biotech. HA powder was obtained from Nanjing Emperor Nano Material Co., Ltd. GO was purchased from Chengdu Organic Chemicals Co. Ltd, China (thickness: 0.55–1.2 nm diameter: 0.5–3 μm). Bovine serum albumin (BSA) was obtained from Beijing Solarbio Science & Technology Co., Ltd. Dichloromethane (DCM) was obtained from Beijing Chemical Works. 1,1,1,3,3,3-hexafluoroisopropanol (HFIP), 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2- H-tetrazolium bromide (MTT) and BCA protein assay kit were purchased from Sigma-Aldrich (USA). Cetylpyridinium chloride (CPC) was purchased from Aladdin Chemistry Co. Ltd. The reagents for cell experiments were purchased from Gibco (USA).
7
HCC HepG2 Cell Culture Protocol
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Human HCC HepG2 cells were purchased from the National Cell Bank of Iran-Pasteur Institute and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 100 mL/L fetal bovine serum, 100 U/mL penicillin, 100 U/mL streptomycin at 37°C in a humidified atmosphere containing 50 mL/L CO2. TSA and GE were purchased from Sigma (St. Louis, MO, USA) and dissolved in dimethyl sulfoxide (DMSO; Sigma) at a final concentration of 100 µM in order to prepare a stock solution. By diluting the stock solution, all of the other test concentrations were prepared. DMEM, DMSO, 3-[4,5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide (MTT) and phosphate-buffered saline (PBS) were purchased from Sigma. Total RNA extraction kit (TRIZOL reagent) and real-time polymerase chain reaction (PCR) kits (qPCR MasterMix Plus for SYBR Green I dNTP) were obtained from Applied Biosystems Inc. (Foster, CA, USA).
8
Evaluation of ERL Cytotoxicity and Mechanism
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N‐acetylcysteine (NAC), Hoechst 33342 stain, 3‐(4, 5‐dimethyl‐2‐thiazolyl)‐2, 5‐diphenyl‐2‐H‐tetrazolium bromide (MTT), 5, 5′, 6, 6′‐tetrachloro‐1, 1′, 3, 3′‐tetraethyl‐imidacarbocyanine iodide (JC‐1), and carbonyl cyanide 3‐chlorophenylhydrazone (CCCP) were purchased from Sigma‐Aldrich (St. Louis, USA). ERL was purchased from Sigma Chemicals and dissolved in dimethyl sulfoxide (DMSO) to produce a stock solution. A working solution was diluted from the stock solution using cell culture medium. All antibodies were purchased from (Sigma‐Aldrich, St. Louis, USA).
9
Apoptosis Induction in KG1-a Cells
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From the Pasteur Institute of Iran (Tehran) was provided KG1-a cell line. Fetal bovine serum (FBS) and cell culture media (RPMI 1640) were bought from BioSolutions International (Melbourne, Australia). We bought penicillin and streptomycin from Invitrogen (Grand Island, NY, USA). The caspase-3 test kit and the annexin V-FITC/PI apoptosis detection kit were purchased from BD Biosciences Pharmingen (San Diego, CA, USA). POCH supplied the dimethyl sulfoxide (DMSO) (Gliwice, Poland). Sigma Aldrich provided the following chemicals: Hoechst 33342, 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT), and propidium iodide (PI) (St. Louis, Missouri, USA). Oligo produced qPCR primers (Macrogen, Seoul, South Korea). We bought the RNA isolation kit from SinaClon (Iran). Prime Script RT reagent kit (Takara, Japan) was used for cDNA synthesis and real-time PCR, while AmpliQon provided RealQ Plus 2 Master Mix Green High ROXTM (AmpliQon, Denmark).
10
Culturing Human Colon Cancer Cell Lines
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Human colon cancer SW 480 and SW 948 cell lines were purchased from the National Cell Bank of Iran-Pasteur Institute and cultured and maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with fetal bovine serum 10% and antibiotics in a humidified atmosphere of 5% CO2 in air at 37°C. 5-aza-CdR was provided from Sigma (St. Louis, MO, USA) and dissolved in dimethyl sulfoxide (DMSO; Sigma) at a final concentration of 100 μM to obtain a stock solution. All other working solutions were provided by diluting the stock solution. Antibiotics, DMSO, trypsin-EDTA, 3-[4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide (MTT), Annexin-V-(FITC), propidium iodide (PI), DMEM, and Phosphate-buffered saline (PBS) were purchased from Sigma. Total RNA extraction kit (TRIZOL reagent) and real-time polymerase chain reaction (PCR) kits (qPCR MasterMix Plus for SYBR Green I dNTP) were obtained from Applied Biosystems Inc. (Foster, CA, USA). This work was approved in the Ethics Committee of Jahrom University of Medical science with a code number of IR. JUMS.REC.1397.138.
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