Changes in oligomerization in response to mutations and complex formation were monitored by native (non-denaturing) PAGE on a PhastSystem system (GE Healthcare) with pre-made 8–25% acrylamide gradient gels (GE Healthcare). Separately purified proteins were pre-incubated at 20 °C for 1 h before loading the gel. Coomassie Brilliant Blue was used for staining and detection of the shifted bands.
Phastsystem system
Manufactured by GE Healthcare
The PhastSystem is a compact and automated electrophoresis system designed for protein separation and analysis. It allows for the rapid and efficient separation of proteins using pre-cast polyacrylamide gels. The PhastSystem provides a standardized and reproducible platform for various electrophoresis techniques, enabling researchers to analyze protein samples with consistent results.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using phastsystem system
1
Monitoring Oligomerization Changes by Native PAGE
2
Recombinant α-Galactosidase Purity Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To examine purity and molecular mass of recombinant α-galactosidases, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE) (10% polyacrylamide) was used (Witte et al., 2010 (link)). Samples were run under reduced conditions. After SDS-PAGE analysis, gels were stained with Coomassie Brilliant Blue staining or silver stain using the PhastSystem system (GE Healthcare).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!