Cell proliferation was determined by the MTS [3-(4, 5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] (CellTiter96; Promega) assay. Cells were plated into 96-well plates (3000 cells per well) and incubated with MTS at 37 °C for 4 h. The optical density (OD) at 490 nm was determined with a microplate reader (Bio-Tek EL-311; BioTek Instruments Inc., Winooski, USA). Results are representative of three independent experiments with triplicate samples for each cell line.
Bio tek el 311
Manufactured by Agilent Technologies
Sourced in United States
The Bio-Tek EL-311 is a microplate reader designed for absorbance-based measurements. It is capable of reading microplates with 6 to 384 wells. The device uses a tungsten-halogen lamp as the light source and features a monochromator for wavelength selection. It can measure absorbance in the range of 340 to 750 nanometers.
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Lab products found in correlation
3 protocols using bio tek el 311
1
MTS Assay for Cell Proliferation
2
Cell Proliferation Assay with Vorinostat and Cisplatin
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Cell proliferation was determined using the CellTiter 96TM Aqueous non-radioactive cell proliferation kit (Promega) according to manufacturer instructions. Approximately 5000 cells were plated into 96-well plates in quintuplicate. Cells were treated with control (vehicle), Vorinostat (0.1, 0.25, 0.5, 1.0, 2.5, 5.0 and 10.0 μM), or cisplatin (2.5,5.0, 7.5, 10.0, 12.5, 15.0, 17.5 and 20.0 μg/ml) for 24 h. Cells were incubated with MTS at 37 °C for 4 h, and the results were assessed by absorbance (Bio-Tek EL-311, Bio-Tek Instruments) at 490 nm.
3
Cell Proliferation Assay with Vorinostat and Cisplatin
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