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Slug 9585

Manufactured by Cell Signaling Technology
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Slug (#9585) is a lab equipment product from Cell Signaling Technology. It is a protein involved in the regulation of cell migration and epithelial-mesenchymal transition.

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Lab products found in correlation

α tubulin sc 23948, by Santa Cruz Biotechnology (2 mentions) Erk1 2 9102, by Cell Signaling Technology (2 mentions) Smad3 9523, by Cell Signaling Technology (2 mentions) E cadherin, by BD (2 mentions) P smad2 3108, by Cell Signaling Technology (2 mentions) Smad2 3103, by Cell Signaling Technology (2 mentions) N cadherin, by BD (2 mentions) β actin, by Merck Group (2 mentions) Akt 4691, by Cell Signaling Technology (2 mentions) Anti mouse 5470 and anti rabbit 5151 secondary antibodies, by Cell Signaling Technology (2 mentions) P38 9212, by Cell Signaling Technology (2 mentions) P smad3 9520, by Cell Signaling Technology (2 mentions) P akt 4058, by Cell Signaling Technology (2 mentions) Perk1 2 9101, by Cell Signaling Technology (2 mentions) Sb431542, by Merck Group (1 mentions) Recombinant human gdf 8, by R&D Systems (1 mentions) Tgf β1, by R&D Systems (1 mentions) Resveratrol, by Merck Group (1 mentions) Star sc 166821, by Santa Cruz Biotechnology (1 mentions) U0126, by Cayman Chemical (1 mentions)

5 protocols using slug 9585

1

Quantification of Epithelial-Mesenchymal Markers

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The MMP2 (#40994), SMAD4 (#38454), Snail (#3895), and Slug (#9585) antibodies were obtained from Cell Signaling Technology. The α-tubulin (#sc-23948) antibody was obtained from Santa Cruz Biotechnology. The recombinant human GDF-8 and TGF-β1 were obtained from R&D systems. The SB431542 was obtained from Sigma.
Chen J., Song T., Yang S., Meng Q., Han X., Wu Z., Cheng J.C, & Fang L. (2023). Snail mediates GDF-8-stimulated human extravillous trophoblast cell invasion by upregulating MMP2 expression. Cell Communication and Signaling : CCS, 21, 93.
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2

TGF-β Signaling Pathway Activation

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Cells were serum-starved overnight and treated with 100pM TGF-β for 60 minutes. Cells were lysed in 2× Laemmli sample buffer, and the proteins were resolved on a 10% SDS-PAGE gel. All primary antibodies (Slug #9585, p-Smad2 #3108, Smad2 #3103, p-Smad3 #9520, Smad3 #9523, p-ERK1/2 #9101, ERK1/2 #9102, p-AKT #4058, AKT #4691, p-p38 #4631, p38 #9212) were purchased from Cell Signaling (Danvers, MA, USA) except for β-actin (Sigma, AA5441) and N-cadherin (#610921) and E-cadherin (#610182) (BD Biosciences). Anti-mouse (#5470) and anti-rabbit (#5151) secondary antibodies were purchased from Cell Signaling. Blots were scanned using the LI-COR Odyssey (LI-COR Biosciences, Lincoln, NE, USA).
Huang J.J., Corona A.L., Dunn B.P., Cai E.M., Prakken J.N, & Blobe G.C. (2019). Increased type III TGF-β receptor shedding decreases tumorigenesis through induction of epithelial-to-mesenchymal transition. Oncogene, 38(18), 3402-3414.
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3

TGF-β Signaling Pathway Activation

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Cells were serum-starved overnight and treated with 100pM TGF-β for 60 minutes. Cells were lysed in 2× Laemmli sample buffer, and the proteins were resolved on a 10% SDS-PAGE gel. All primary antibodies (Slug #9585, p-Smad2 #3108, Smad2 #3103, p-Smad3 #9520, Smad3 #9523, p-ERK1/2 #9101, ERK1/2 #9102, p-AKT #4058, AKT #4691, p-p38 #4631, p38 #9212) were purchased from Cell Signaling (Danvers, MA, USA) except for β-actin (Sigma, AA5441) and N-cadherin (#610921) and E-cadherin (#610182) (BD Biosciences). Anti-mouse (#5470) and anti-rabbit (#5151) secondary antibodies were purchased from Cell Signaling. Blots were scanned using the LI-COR Odyssey (LI-COR Biosciences, Lincoln, NE, USA).
Huang J.J., Corona A.L., Dunn B.P., Cai E.M., Prakken J.N, & Blobe G.C. (2019). Increased type III TGF-β receptor shedding decreases tumorigenesis through induction of epithelial-to-mesenchymal transition. Oncogene, 38(18), 3402-3414.
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4

Signaling Pathways in Cellular Processes

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The p-ERK1/2 (#9106), ERK1/2 (#9102), p-AKT (#9271), AKT (#9272), Snail (#3895), and Slug (#9585) antibodies were purchased from Cell Signaling Technology. The StAR (#sc-166821) and α-tubulin (#sc-23948) antibodies were purchased from Santa Cruz Biotechnology. The StAR anti-body only detects the mature form of StAR (30-kDa). The GPER (#ab154069) antibody was purchased from Abcam. The resveratrol was obtained from Sigma. The G-15 and U0126 were obtained from Cayman.
Song T., Chen J., Yang S., Liu B., Zhang L., Zhang Q., Cheng J.C, & Fang L. (2023). Resveratrol stimulates StAR expression and progesterone production by GPER-mediated downregulation of Snail expression in human granulosa cells. Journal of Food and Drug Analysis, 31(2), 315-325.
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5

Western Blot Analysis of Protein Markers

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Proteins were extracted as previously described. 30 Protein (20 µg of total protein lysate per sample) was subjected to western blot (WB) analysis using standard protocols. EZH2 (#5246) and SLUG (#9585) antibodies were purchased from Cell Signaling Technology (USA); YY1 (ab109237), integrinβ3 (ab75872) and c-myc (ab32072) antibodies were obtained from Abcam (USA). Actin (A2228), used to normalize protein expression, was purchased from Sigma Aldrich (Italy). The secondary antibodies anti-rabbit and anti-mouse were purchased from Santa Cruz Biotechnology (USA).
Dagrada G., Rupel K., Zacchigna S., Tamborini E., Pilotti S., Cavalleri A., Fechner L.E., Laurini E., Smith D.K., Brich S, & Pricl S. (2018). Self-Assembled Nanomicelles as Curcumin Drug Delivery Vehicles: Impact on Solitary Fibrous Tumor Cell Protein Expression and Viability. Molecular pharmaceutics, 15(10).
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