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Impact 2 esi qtof

Manufactured by Bruker
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Sourced in Germany, United States

The Impact II ESI-QTOF is a high-resolution mass spectrometer designed for accurate mass measurement and confident compound identification. It features an electrospray ionization (ESI) source and a quadrupole time-of-flight (QTOF) mass analyzer.

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Lab products found in correlation

Nanoelute column, by Bruker (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Dionex ultimate 3000, by Thermo Fisher Scientific (1 mentions) Lys c, by Promega (1 mentions) Glu c, by Promega (1 mentions) Dataanalysis, by Bruker (1 mentions) Myc trap beads, by Proteintech (1 mentions) Ms grade trypsin, by Thermo Fisher Scientific (1 mentions)

5 protocols using impact 2 esi qtof

1

Proteomic Analysis of Biological Samples

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Samples were analyzed on a Bruker Impact II ESI-QTOF (Bruker Daltonics, Bremen, Germany) mass spectrometer with an on-line Dionex Ultimate 3000 chromatography system (Thermo Fisher Scientific, Waltham, Massachusetts, U.S.A.) equipped with a Bruker Nanoelute column (15 cm, 75 µm ID). Peptides were eluted using a solvent gradient over 65 min, using acetonitrile with 0.1% formic acid at a flow rate of 300 nl min−1. The MS scan range was 150–2200 m/z with a cycle time of 3 min using a MS sampling rate of 2 Hz followed by intensity-based data-dependent MS/MS (4–16 Hz).
Pinheiro-Machado E., Milkewitz Sandberg T.O., PIHL C., Hägglund P.M, & Marzec M.T. (2020). In silico approach to predict pancreatic β-cells classically secreted proteins. Bioscience Reports, 40(2), BSR20193708.
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2

Nitrative Stress Proteome Analysis

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AN (25 μL, 5 μM) exposed to ONOOH as described above (section 2.3) was mixed with 75 μL 8 M urea (in 100 mM Tris, pH 8.0) and 1 μL LysC (20 ng μL−1; Promega) then incubated at 21 °C for 4 h. Subsequently 150 μL 100 mM Tris (pH 8.0), 500 μL H2O and 2 μL GluC (20 ng μL−1; Promega) were added, and the samples incubated overnight at 21 °C. Following solid phase extraction with Stage-tips, samples were analyzed on a Bruker Impact II ESI-QTOF (Bruker Daltonics) mass spectrometer in the positive ion mode with a Captivespray ion source connected on-line to a Dionex Ultimate 3000RSnano chromatography systems (Thermo Fisher Scientific). Peptides were separated on a Bruker Nanoelute column (15 cm × 75 μm ID) with a solvent gradient over 65 min, using acetonitrile with 0.1% formic acid at a flow rate of 300 nL min−1. The MS scan range was 150–1750 m/z with a cycle time of 2 s using MS and MS/MS sampling rates of 2 Hz. Database searches were performed with MaxQuant v1.6.1.0 using the following parameters: enzyme: GluC and LysC, with three missed cleavages; variable modification: Tyr and Trp nitration (+44.985 Da) and dinitration (89.97 Da); mass tolerance: 0.07 and 0.005 Da (first and main searches, respectively); MS/MS mass tolerance: 40 ppm (first and main searches).
He J., Becares E.R., Thulstrup P.W., Gamon L.F., Pedersen J.N., Otzen D., Gourdon P., Davies M.J, & Hägglund P. (2020). Peroxynitrous acid (ONOOH) modifies the structure of anastellin and influences its capacity to polymerize fibronectin. Redox Biology, 36, 101631.
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3

Protein Desalting and Mass Spectrometry

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2 μl of protein preparations at 10 μM were desalted on a Shim-pack Scepter C4-300 (G) column (3 μM) by washing with 1% ACN in the presence of 0.1% formic acid. Subsequently, increasing concentrations of acetonitrile (1–95%) eluted the proteins into an Impact II ESI-Q-TOF (Bruker) mass spectrometer. Protein signatures in the mass spectra were integrated and deconvoluted using the maximum entropy function of DataAnalysis (Bruker).
Tran Q.H., Eder O.M, & Winkler A. (2024). Dynamics-driven allosteric stimulation of diguanylate cyclase activity in a red light-regulated phytochrome. The Journal of Biological Chemistry, 300(5), 107217.
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4

GRP94 and FKBP2 Interactome Analysis

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INS-1E cells were transfected at 60% confluence using Lipofectamine™ 3000 (ThermoFisher Scientific, Denmark) with plasmids coding for GFP-tagged GRP94, PI, ER-localized GFP [23 (link),24 (link)] or myc-tagged FKBP2, according to the manufacturer’s protocol. Proteins were immunoprecipitated (IP) using the magnetic GFP- or myc-trap beads (Chromotek, Germany). IP samples were reduced and alkylated, digested with trypsin/LysC and the resulting peptides were analyzed on a Bruker Impact II ESI-QTOF (Bruker Daltonics, USA) mass spectrometer (supplementary procedures).
Hoefner C., Bryde T.H., Pihl C., Tiedemann S.N., Bresson S.E., Hotiana H.A., Khilji M.S., Santos T.D., Puglia M., Pisano P., Majewska M., Durzynska J., Klindt K., Klusek J., Perone M.J., Bucki R., Hägglund P.M., Gourdon P.E., Gotfryd K., Urbaniak E., Borowiak M., Wierer M., MacDonald P.E., Mandrup-Poulsen T, & Marzec M.T. (2023). FK506-Binding Protein 2 Participates in Proinsulin Folding. Biomolecules, 13(1), 152.
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5

UPLC-MS/MS Tryptic Peptide Analysis

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After the samples were trypsinized with 0.0044 μg/μL MS grade trypsin (Thermo Fisher Scientific, PI90057) with an incubation of at least 4 h at 37 °C, the samples were run on the Bruker Impact II ESI QTOF. Samples were first passed through an ACUITY UPLC Peptide BEH C18 column (300 Å, 1.7 μM, 2.1 mm × 100 mM, 186003686 Waters Corp.) equipped with a 10 mm guard column of identical packing (186004629 Waters Corp.). Then, the samples were eluted at a flow rate of 0.5 mL/min and a 40 °C column temperature starting at a 1 min hold of 95% solvent A (100% water and 0.1% formic acid) and 5% solvent B (100% acetonitrile and 0.1% formic acid) with a linear gradient up to 0% solvent A and 100% solvent B for a total run time of 9 min.
Thames A.H., Moons S.J., Wong D.A., Boltje T.J., Bochner B.S, & Jewett M.C. (2023). GlycoCAP: A Cell-Free, Bacterial Glycosylation Platform for Building Clickable Azido-Sialoglycoproteins. ACS synthetic biology, 12(4), 1264-1274.
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