Tcs sp5 x aobs

3-nitro-1,8-napthalimide and formic hydrazide were purchased from Sigma-Aldrich and used as received. The solvents used for synthesis and photophysical studies were of spectroscopic grade. For photophysical studies double-distilled water was used and pH of water was 6.7 and conductivity was 30 dS m⁻¹. For stock solution of all anions tetrabutylammonium salts were purchased and for all cations, chloride salts were used. Bruker DRX-300, Agilent Unity INOVA-500 and Bruker Ascend 500 NMR spectrometer were used for NMR spectral characterization. UV-Vis spectra were obtained using Agilent 8453 UV-Vis spectrometer and Shimadzu UV-2600 spectrophotometer. Fluorescence spectra measurements were obtained from Hitachi F-7000 fluorescence spectrophotometer and Shimadzu RF5301pc spectrofluorophotometer. The potentiometric titration was measured using Bio-logic SP-150 cyclic-voltammeter (CV). Lifetime measurement studies were done at Horiba Delta time instrument coupled with TCSPC. Fluorescent images were taken on a Leica TCS SP5 X AOBS and Leica TCS SP5 II Confocal fluorescence microscope. The cells RAW264.7 were provided by the Food Industry Research and Development Institute (Taiwan). The cell imaging and zebrafish imaging experiments were conducted in strict accordance with the guidelines provided by Govt. of Taiwan and was approved by Institutional committee (NCTU, Taiwan).

The cells were seeded on a 35 mm Petri dish at a density of 5 × 10⁴ cells per well and cultured for 24 h. TPE samples of various concentrations (10–50 μM) were treated with 3A6 cells and incubated for 24 hours. After staining for suitable time, the cells were washed with phosphate-buffered saline for three times (pH = 7.4) and images were acquired using laser-confocal microscope (Leica TCS-SP5-X AOBS).