Plasma clinical biochemistry was analyzed as described in detail previously46 (link) biochemical parameters were measured using an Olympus AU640 automatic analyzer. The measurements for each of the samples for biochemical parameters were replicated 3 times.
Au640 automatic analyzer
Manufactured by Olympus
Sourced in Japan
The AU640 automatic analyzer is a clinical chemistry analyzer designed for high-throughput laboratory testing. It is capable of performing a wide range of biochemical assays, including quantitative analysis of various analytes in clinical samples. The AU640 utilizes advanced analytical techniques to deliver reliable and accurate results, supporting the diagnostic needs of healthcare professionals.
Automatically generated - may contain errors
8 protocols using au640 automatic analyzer
1
Plasma Biochemistry Measurement Protocol
2
Metabolic Profile and Kidney Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After three weeks, the rats were housed individually in metabolic cages for 24 h urinary collection and the body weight was measured. The kidney weight index was calculated by kidney weight/body weight. BUN, Scr, cholesterol, triglyceride, uric acid, potassium, sodium, chloridion, phosphorus, calcium and creatine kinase were measured using an Olympus AU640 automatic analyzer and Easylyte plus Analyzer. Plasma aldosterone concentration was measured using an Aldosterone Kit (Shanghai Meilian Biological Technology Co., LTD., Shanghai, China) following the manufacturer's instructions. Blood parameters were determined by HF-3800 analyzer.
3
Protein Expression Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood analyses were performed on an Olympus AU640 automatic analyzer. The histological protocol was described in detail previously [12 (link)]. The Western blot protocol was described previously [13 (link)]. The following primary antibodies were used: anti-collagen I (ab34710, Abcam, Cambridge, UK), anti-fibronectin (ab45688, Abcam), anti-α-SMA (ab124964, Abcam), anti-GAPDH (HRP-60004, Proteintech, Rosemont, IL, USA), anti-PPAR-γ (ab209350, Abcam), anti-SREBP-1 (ab28481, Abcam), anti-TGF-β1 (21898-1-AP, Proteintech), anti-Smad2 (#5339, CST, Danvers, MA, USA), anti-Smad3 (#9523, CST), anti-Smad7 (MAB2029, R&D, Minneapolis, MN, USA), anti-β-catenin (#8480, CST), anti-12-LO (C-5) (sc-365194, Santa Cruz, Santa Cruz, CA, USA), anti-Rac1 (66122-1-Ig, Proteintech), and anti-NOX4 (14347-1-AP, Proteintech) antibodies, and the Wnt Signaling Antibody Sampler Kit (#2915, CST). Semiquantitative analysis of each protein was performed by using ImageJ software (version 1.48), and the band densities were normalized to those of GAPDH.
4
Metabolic and Organ Analysis in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the end of the experiment, rats were housed individually for 24 h in metabolic cages for urinary collection. Body weights were recorded after the 24 h time-period. Upon collection of their urine samples, rats were allowed free access to water. Urine samples were stored at −80°C prior to analysis, and kidneys were weighed to calculate organ indexes (organ index = organ weight/body weight ×100%). Serum levels of creatinine, urea, cholesterol, triglyceride, low density lipoprotein-cholesterol (LDL-C), and high density lipoprotein-cholesterol (HDL-C) were measured using an Olympus AU640 automatic analyzer.
5
D-Dimer Assay for Blood Clot Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Patients’ blood samples were obtained at presentation and processed immediately. The D-dimer was assayed with a latex-enhanced immunoturbidimetric assay in the clinical laboratory of the Shanghai Cancer Center using an Olympus AU640 automatic analyzer (Olympus Corporation, Tokyo, Japan). Commercially available reagents were used to measure the D-dimer levels (Daiichi Seiyaku Co Ltd, Tokyo, Japan). The reference value for D-dimer was less than 0.5 μg/mL. The normal plasma levels of D-dimer range from 0 to 0.5 μg/mL. Hence, a serum plasma D-dimer concentration of >0.5 μg/mL was considered positive.
6
Routine Lipid Profile Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum TC, TG, LDL-C, and HDL-C levels were measured using an AU640 automatic analyzer (Olympus, Tokyo, Japan) according to the manufacturer's instructions.
7
Plasma Biochemistry Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma biochemistry was analyzed as described in detail previously (Zhao et al., 2013c (link)). Biochemical parameters were measured using an Olympus AU640 automatic analyzer.
8
Renal Function and Inflammation Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Baseline information included sex, age, body mass index (BMI), blood pressure, primary renal diseases, and medication histories. Blood samples were obtained after an overnight fasting. Serum was immediately separated by centrifugation and stored at -80 °C. The eGFR was calculated using the modified equation of Diet of Renal Disease. Serum biochemistry was determined by Olympus AU640 automatic analyzer. Serum high sensitivity C-reactive protein (CRP) was measured by an automated immunoturbidimetric assay. Serum interleukin-6 and tumor necrosis factor-α (TNF-α) were measured using the commercially available ELISA kits.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!