PD173074 (Sigma-P2499, HPLC ≥ 96%) and olaparib (AZD2281/KU0059436, #S1060, HPLC ≥ 99.7%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA) and Selleck Chemicals (Antibody International Inc. Jhubei City, Hsinchu County, Taiwan), respectively. Stock solutions (1 mM) of each drug were prepared by dissolution in phosphate-buffered saline (PBS) and stored in a dark room at −20 °C. PBS, dimethyl sulfoxide (DMSO), sulforhodamine B (SRB) reagent, trypsin/ethylenediaminetetraacetic acid, Tris aminomethane (Tris) base, and acetic acid were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Invitrogen (Invitrogen Life Technologies, Carlsbad, CA, USA).
Tris aminomethane tris base
Manufactured by Merck Group
Sourced in United States
Tris aminomethane (Tris) base is a chemical compound used as a pH buffer in various laboratory applications. It is a white crystalline solid that is highly soluble in water and commonly used to maintain a stable pH environment in biological, biochemical, and analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Sulforhodamine b srb reagent, by Merck Group (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Trypsin ethylenediaminetetraacetic acid, by Merck Group (1 mentions)
Trypsin ethylenediaminetetraacetic acid trypsin edta, by Merck Group (1 mentions)
2 protocols using tris aminomethane tris base
1
Cytotoxicity evaluation of PD173074 and olaparib
2
BX-795 Hydroxide Compound Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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BX-795 hydroxide (#SML0694, HPLC ≥ 98%) was purchased from Sigma Aldrich Co. (St. Louis, MO, USA). Stock solutions of 1 mM were dissolved in dimethyl sulfoxide (DMSO) at 15 mg/mL, and stored in dark room at −20 °C. Phosphate buffered saline (PBS, #P7059), dimethyl sulfoxide (DMSO, #D2650), sulforhodamine B (SRB) reagent (#230162), trypsin/ethylenediaminetetraacetic acid (Trypsin-EDTA, #T4049) solution, trisaminomethane (Tris) base (#93352) and acetic acid (#695092) were purchased from Sigma Aldrich Co. (St. Louis, MO, USA), while GibcoTM Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Invitrogen (#11966025, Invitrogen Life Technologies, Carlsbad, CA, USA).
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