Mice were killed at the indicated times, and pulmonary homogenates were lysed in RIPA lysis buffer (Beyontime, China). Lysates were stored at −80 °C. Cytokine levels were detected using a mouse ELISA kit (Solarbio, Beijing) and read on a Luminex 100 (Bio‐Rad), as described in the manufacturer’s instructions.
Mouse elisa kit
Manufactured by Solarbio
Sourced in China
The Mouse ELISA kit is a laboratory equipment designed for the quantitative measurement of specific proteins or analytes in mouse biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte. The kit provides the necessary reagents and protocols to perform the assay.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Luminex 100, by Bio-Rad (1 mentions)
Ab197742, by Abcam (1 mentions)
Ab222503, by Abcam (1 mentions)
Ab199076, by Abcam (1 mentions)
Ab208348, by Abcam (1 mentions)
Microplate reader, by Molecular Devices (1 mentions)
Mouse lipopolysaccharides lps elisa kit, by Cusabio (1 mentions)
7 protocols using mouse elisa kit
1
Pulmonary Cytokine Profiling in Mice
2
Mouse Serum Biomarker Quantification
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To measure mouse serum HMGB1, IL-1α, IL-1β, IL-6, TNF-α and oxidative 8-OHdG DNA damage we used the mouse ELISA kit (Cat#SEKM-0145, Solarbio Science & Technology, Beijing, China), IL-1α (ab199076, Abcam, Cambridge, MA), IL-1β (ab197742, Abcam, Cambridge, MA), IL-6 (ab222503, Abcam, Cambridge, MA), TNF-α (ab208348, Abcam, Cambridge, MA), and oxdative 8-OHdG DNA damage (8-OHdG Quantitation) (STA-210-T, Cell biolabs, San Diego, CA) according to each manufacturer’s instructions. Absorbance levels of concentrations were measured at 450 nm using a microplate reader (Molecular Devices, Sunnyvale, CA).
3
Investigating Inflammatory Response to OP-I Exposure
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RAW264.7 cells were seeded in a 96-well cell-culture plate and treated with 100 µL of the P0OP-I or P15OP-I solution at gradient concentrations (100, 50, 25, 12.5, 6.25, and 0 µg/mL) for 24 h. NO in the culture supernatant was detected using an NO Reagent Assay Kit (Elabscience Biotechnology Co., Ltd., Wuhan, China). TNF-α in the culture supernatant was detected following the ELISA method with a Mouse ELISA kit (Solarbio, Beijing, China). Detection was performed following the manufacturers’ protocols.
4
MP3RT-specific IgG Quantification
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Blood samples were collected from the mice when they were sacrificed on day 91 after the first immunization. The collected blood sample was centrifuged at 1500rpm for 20 min, and then the supernatant was gently transferred into a new tube. The serum separated from each blood sample was linearly diluted (2 times) with PBS from a minimum dilution of 100 to a maximum dilution of 204800 to determine the optimum dilution by a Mouse ELISA Kit (Solarbio, Beijing, China) following the instruction given by the manufacturer. After that, the rest of the serum was diluted in the optimal dilution, and the levels of MP3RT specific IgG were determined by Goat anti-Mouse IgG/HRP (Cat. No. SE131, Solarbio, Beijing, China). Finally, the OD450 values of MP3RT specific IgG were detected with a microplate reader (Thermo Fisher Scientific, Shanghai, China).
5
Serum Cytokine Quantification by ELISA
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IL-1β and IL-6 in serum obtained on the last day were measured by mouse ELISA kit (Solarbio, China). The assays were executed according to the manufacturers' instructions. The absorbance of the specimen was detected at 450 nm by a microplate reader.
6
ATDC5 Cell Cytokine Analysis
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After the indicated treatment, culture supernatant of ATDC5 cells was collected from 6-well plates, and the concentrations of TNF-α, IL-1β, IL-6, and iNOS were, respectively, measured by using a mouse ELISA Kit (Solarbio, Beijing, China) according to the manufacturer's instructions.
7
Biomarkers of Liver Injury and Inflammation
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Serum aspartate transaminase (AST) and alanine transaminase (ALT) were measured using the In nity ALT Kit (Thermo Fisher Scienti c). Serum LPS was determined using the Mouse Lipopolysaccharides (LPS) ELISA Kit (Cusabio, Wuhan, China). The serum levels of TNF-α, IL-1β, and IL-6, as well as the hepatic triglyceride (TG) and hepatic lipid peroxidation (MDA) level, were determined using a Mouse ELISA Kit (Solarbio, Beijing, China). All the assays were performed in triplicate according to the manufacturer's instructions.
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