Anti gfp clones 7 1 and 13

Manufactured by Roche

Anti-GFP clones 7.1 and 13.1 are monoclonal antibodies that specifically recognize and bind to green fluorescent protein (GFP). They are useful tools for the detection and localization of GFP-tagged proteins in various applications, such as western blotting, immunoprecipitation, and immunocytochemistry.

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Lab products found in correlation

Goat anti mouse igg h l hrp conjugate, by Bio-Rad (2 mentions) Anti py397 fak, by Thermo Fisher Scientific (1 mentions) Anti phosphotyrosine clone 4g10 05 321, by Merck Group (1 mentions) Goat anti rabbit 800cw, by LI COR (1 mentions) Goat anti mouse 680lt, by LI COR (1 mentions) Rhodamine labeled phalloidin, by Thermo Fisher Scientific (1 mentions) Alexa fluor conjugated secondary, by Thermo Fisher Scientific (1 mentions) Anti cortactin ab 33333, by Abcam (1 mentions) Anti arp2 h 84 sc 15389, by Santa Cruz Biotechnology (1 mentions) Anti tks5 fish m 300 sc 30122, by Santa Cruz Biotechnology (1 mentions) Anti fak clone 77 610088, by BD (1 mentions) Anti β actin clone ac 15 a5441, by Merck Group (1 mentions) Nupage 4 12 bis tris protein gel, by Thermo Fisher Scientific (1 mentions) Anti actin, by Merck Group (1 mentions) Myc tag 9b11, by Cell Signaling Technology (1 mentions) Anti flag m2 peroxidase hrp clone m2, by Merck Group (1 mentions) Nitrocellulose membrane, by GE Healthcare (1 mentions) Anti pgk, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase conjugated anti mouse immunoglobulin g, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-GFP (254 citations) Mouse anti-GFP (clones 7.1 and 13.1, (4 citations) Anti-GFP (7.1 and 13.1, (3 citations) Anti-GFP 7.1/13.1 (3 citations) GFP (7.1/13.1) (2 citations) Anti-GFP antibody from mouse IgG1κ (clones 7.1 and 13.1) (2 citations) Anti-GFP from mouse IgG1κ (clones 7.1 and 13.1) (2 citations)

4 protocols using anti gfp clones 7 1 and 13

Immunofluorescence and Western Blotting Assay

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For immunofluorescence, anti-cortactin (ab-33333) was obtained from Abcam; anti–pY402-Pyk2 and anti–pY397-FAK were obtained from Invitrogen; anti-Arp2 (H-84; SC-15389) and anti-Tks5 (FISH M-300; SC-30122) were obtained from Santa Cruz Biotechnology, Inc.; and antivinculin (clone hVIN1; V9131), anti–pY421-cortactin (C0739), and anti–pY466-cortactin (C0864) were obtained from Sigma-Aldrich. Rhodamine-labeled phalloidin and Alexa Fluor–conjugated secondary antibodies were obtained from Molecular Probes. For Western blotting, anticortactin (clone 4F11; 05-180) and antiphosphotyrosine (clone 4G10; 05-321) were obtained from EMD Millipore; anti-Pyk2 (3480 and 3292) was obtained from Cell Signaling Technology; anti-FAK (clone 77; 610088) was obtained from BD; anti-GFP (clones 7.1 and 13.1; 11814460001) was obtained from Roche; and anti–β-actin (clone AC-15; A5441) was obtained from Sigma-Aldrich. Secondary antibodies (goat anti–mouse 680LT and goat anti–rabbit 800CW) were obtained from LI-COR Biosciences.

Genna A., Lapetina S., Lukic N., Twafra S., Meirson T., Sharma V.P., Condeelis J.S, & Gil-Henn H. (2018). Pyk2 and FAK differentially regulate invadopodia formation and function in breast cancer cells. The Journal of Cell Biology, 217(1), 375-395.

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Western Blot Analysis of Tagged Proteins

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Frozen 6-day-old seedlings were disrupted in a ball mill using metal
beads. Ground tissue was boiled in 2× NuPAGE LDS Sample Buffer (including
1.8% β-mercaptoethanol) for 5 min and separated in NuPAGE 4–12%
Bis–Tris Protein Gels (ThermoFisher Scientific). The following antibodies
were used for immunoblotting: Myc-Tag, 9B11 (1:2,000, catalog no. 2276, Cell
Signaling Technology), anti-HA-peroxidase, high-affinity clone 3F10 (1:2,000,
catalog no. 11867423001, Roche), anti-FLAG M2-peroxidase (HRP) Clone M2
(1:5,000, catalog no. A8592, MilliporeSigma), anti-GFP clones 7.1 and 13.1
(1:5,000, catalog no. 11814460001, Roche), anti-Actin (1:5,000, catalog no.
A0480, MilliporeSigma) and goat anti-mouse IgG (H + L)-HRP conjugate (1:5,000,
catalog no. 1706516, Bio-Rad).

Willige B.C., Zander M., Yoo C.Y., Phan A., Garza R.M., Wanamaker S.A., He Y., Nery J.R., Chen H., Chen M., Ecker J.R, & Chory J. (2021). PHYTOCHROME-INTERACTING FACTORs trigger environmentally responsive chromatin dynamics in plants. Nature genetics, 53(7), 955-961.

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Protein Extraction and Immunoblotting

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Frozen tobacco leaf discs were disrupted in a ball mill using glass beads. Ground tissue was boiled in sample buffer and separated in SDS-PAGEs. Following antibodies were used in this study: Anti-HA-Peroxidase, High Affinity clone 3F10 (1:2000, Roche, 11867423001), Anti-FLAG M2-Peroxidase (HRP) Clone M2 (1:5000, Sigma-Aldrich, A8592), anti-GFP clones 7.1 and 13.1 (1:5000, Roche, 11814460001) and Goat Anti-Mouse IgG (H + L)-HRP Conjugate (1:5000, Bio-Rad, 1706516).

Bürger M., Willige B.C, & Chory J. (2017). A hydrophobic anchor mechanism defines a deacetylase family that suppresses host response against YopJ effectors. Nature Communications, 8, 2201.

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Immunoblotting Analysis of Yeast Proteins

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Yeast cells were grown as above-mentioned and crude protein extracts were prepared as previously described (50) . Nitrocellulose membranes (GE Healthcare Life Sciences) were probed with anti-GFP (clones 7.1 and 13.1, Roche) and anti-PGK (yeast 3-phosphoglycerate kinase, Invitrogen) antibodies, used at 1:3000 and 1:10000 dilutions, respectively. Primary antibodies were detected with horseradish peroxidase-conjugated anti-mouse immunoglobulin G (Sigma) by enhanced chemiluminescence.

Barata-Antunes C., Talaia G., Broutzakis G., Ribas D.M.N., Beule P.D., Casal M., Stefan C.J., Diallinas G., & Paiva S. (2021). Interactions of cytosolic termini of the Jen1 monocarboxylate transporter are critical for trafficking, transport activity and endocytosis.

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