2424 els detector

The known cytotoxic agents 1(35 (link)) and seco-5^{40 (link)} were prepared as previously described. These
compounds were coupled
to known trioxolane^{29 (link)} and dioxolane^{30 (link)} intermediates via activated nitrophenyl carbonate
or isocyanate intermediates as we have described previously^{29 (link)−31 (link)} and as further detailed in the Supporting Information.
All compounds tested in cells or animals were judged to be
of >95%
purity as determined using a Waters Micromass ZQTM, equipped with
Waters 2795 separation module, Waters 2996 photodiode array detector
(254 nm), and Waters 2424 ELS detector. Separations were carried out
with an XTerra MS C18, 5 μm, 4.6 mm × 50 mm column, at
ambient temperature (unregulated) using a mobile phase of water–methanol
containing a constant 0.10% formic acid. Representative LC chromatograms
are provided in the Supporting Information.
Mammalian cell lines were maintained in an atmosphere of
5% CO₂ in RPMI 1640 media purchased from HyClone supplemented
with
10% FBS (Gibco), Pen/Strep (1× final concentration, Gemini Bio-Products),
and nonessential amino acids (UCSF Cell Culture Facility). Unless
otherwise noted, cell lines were obtained from ATCC and verified by
STR profiling. Graphing and analysis of data were done using GraphPad
Prism 6 software and Microsoft Excel 2010. Figures were prepared with
Adobe Design Standard CS6 software.

Known compounds were prepared according to literature procedures
as cited in the main text. The syntheses of new compounds 12a–k are described in the Supporting Information. All compounds tested in parasites or mice were
judged to be of >95% purity as assessed using a Waters Micromass
ZQ
4000 equipped with Waters 2795 Separation Module, Waters 2996 Photodiode
Array Detector (254 nm), and Waters 2424 ELS detector. Separations
were carried out with an XBridge BEH C18, 3.5 μm, 4.6 ×
20 mm column, at ambient temperature (unregulated) using a mobile
phase of water–methanol containing a constant 0.10% formic
acid.