Vivoquant ver 4

CrTac:NCr-Foxn1nu mice (Taconic, Rensselaer, NY, USA) were injected sc with 1 × 10⁶ of Panc1, BxPC3 or AsPC1 human pancreatic carcinoma cells (ATCC) in 200 μL of Matrigel^®. After tumors reached 1 cm³, mice were injected IV with 3.7–5.5 MBq (100–150 μCi) of [¹⁸F]FEPPG 1. Static 60 min PET was acquired 30 min after [¹⁸F]FEPPG 1 injection using Inveon micro PET scanner (Siemens, Munich, Germany). PET images were reconstructed using the 3D-OSEM algorithm with three iterations in a 256 × 256 matrix, without attenuation correction (Inveon, Siemens, Munich, Germany) and analyzed using VivoQuant ver 4 (Invicro, Boston, MA, USA).

[¹⁸F] FSPG: 10–20 min after HIFU treatment, B6 mice were injected i.v. with 4.7–5.7 MBq (128–153 μCi) of [¹⁸F] FSPG. 30 min post [¹⁸F] FSPG injection, mice were placed into a 4-mouse bed, and 30 min static PET images were acquired using a micro PET scanner (Siemens, Munich, Germany) followed by microCT (MIlabs, Houten, The Netherlands) on the same bed for anatomical reference.
[¹¹C] topotecan: 10–20 min post-HIFU treatment, B6 mice were placed into a 4-mouse bed and injected i.v. with 5.4–7.9 MBq (146–214 μCi) of [¹¹C] topotecan. 60 min dynamic PET images were acquired using a micro PET scanner (Siemens, Munich, Germany) followed by microCT (MIlabs, Houten, The Netherlands) on the same bed for anatomical reference.
For both [¹⁸F] FSPG and [¹¹C] topotecan studies, immediately after PET/CT scans, mice were euthanized, and their dissected brains were placed into 4% PFA and imaged for 10 min with static PET. Regions of interest (ROI) were manually drawn over the heart and the right brain hemisphere around the area targeted by HIFU using microCT as a reference. All PET images were reconstructed using the 3D-OSEM algorithm with 3-iterations in 256 × 256 matrix (Inveon, Siemens, Munich, Germany) and analyzed using VivoQuant ver 4 (Invicro, Boston, MA, USA).

72 h after intranasal LPS treatment, B6 mice were injected iv with 436–473 μCi (16.1–17.5 MBq) of ⁶⁸Ga-albumin. 4 to 5 h after ⁶⁸Ga-albumin injection, mice were placed into a 4-mouse bed and 30 min static PET images were acquired using micro PET scanner (Siemens, Germany) followed by microCT (MIlabs, Netherlands) on the same bed for anatomical reference. Immediately after PET/CT acquisition, lungs were dissected, placed in 4% PFA in a 6-well culture plate, and a 30 min PET static image of the isolated lungs was acquired. All PET images were reconstructed using 3D-OSEM algorithm with 3-iterations in 256 × 256 matrix (Inveon, Siemens, Germany) and analyzed using VivoQuant ver 4 (Invicro, MA).