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Phospho stat1 tyr 701 alexa fluor 488 conjugated rabbit antibody

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The Phospho-STAT1 (Tyr 701) Alexa Fluor 488 conjugated rabbit antibody is a laboratory reagent used to detect and visualize the phosphorylation of the STAT1 protein at tyrosine 701 in cellular samples. The antibody is conjugated with the Alexa Fluor 488 fluorescent dye, enabling fluorescence-based detection and analysis.

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Lab products found in correlation

F4 80, by Bio-Rad (2 mentions) Mouse anti mrc 1, by Abcam (2 mentions) Gallios flow cytometer, by Cell Signaling Technology (2 mentions) Gata3, by Bio-Rad (2 mentions) Alexa fluor 488 goat anti mouse secondary antibody, by Thermo Fisher Scientific (2 mentions) Cd11b, by Bio-Rad (2 mentions)

Close spelling variants of this product

Phospho-Tyr 701 STAT1 Alexa Fluor 488-conjugated antibody Alexa Fluor 488 antibody Phospho-STAT1 antibody Alexa Fluor 488 Phospho-STAT1 STAT1 antibody Alexa 488 STAT1

2 protocols using phospho stat1 tyr 701 alexa fluor 488 conjugated rabbit antibody

1

Multiparametric Immune Profiling by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed with 4 % PFA and stained with a mouse anti-MRC-1 (Abcam) and a goat-anti mouse Alexa Fluor 488 secondary antibody (Life Technologies). For intracellular staining, cells were fixed with 4% PFA and permeabilized with ice cold methanol. Cells were stained with phospho-STAT1 (Tyr 701) Alexa Fluor 488 conjugated rabbit antibody (Cell Signalling), rabbit anti-SOCS1 (ab135718) and assessed by flow cytometry using the Gallios Flow Cytometer. In addition, the following antibodies from Biolegend were used in this study : CD3 (100235), CD4 (100405), CD8a (100707), CD11b (101207), CD19 (152403), CD45 (103111), CD69 (104507), CD115 (135523), CD206 (MRC-1) (141707), F4/80 (123107), FOXP3 (126403), GATA3 (653805), Gr-1 (108411), MARCO (BioRad ED31), RORγt (654301), T-bet (644809). Antibodies were conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), PE-Cy7 or allophycocyanin (APC) and appropriate isotype controls were included in all experiments.
Subramanian K., Neill D.R., Malak H., Spelmink L., Khandaker S., Marchiori G.D., Dearing E., Kirby A., Yang M., Achour A., Nilvebrant J., Nygren P.Å., Plant L., Kadioglu A, & Henriques-Normark B. (2018). Pneumolysin binds to the Mannose-Receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival. Nature microbiology, 4(1), 62-70.
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2

Multiparametric Immune Profiling by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed with 4 % PFA and stained with a mouse anti-MRC-1 (Abcam) and a goat-anti mouse Alexa Fluor 488 secondary antibody (Life Technologies). For intracellular staining, cells were fixed with 4% PFA and permeabilized with ice cold methanol. Cells were stained with phospho-STAT1 (Tyr 701) Alexa Fluor 488 conjugated rabbit antibody (Cell Signalling), rabbit anti-SOCS1 (ab135718) and assessed by flow cytometry using the Gallios Flow Cytometer. In addition, the following antibodies from Biolegend were used in this study : CD3 (100235), CD4 (100405), CD8a (100707), CD11b (101207), CD19 (152403), CD45 (103111), CD69 (104507), CD115 (135523), CD206 (MRC-1) (141707), F4/80 (123107), FOXP3 (126403), GATA3 (653805), Gr-1 (108411), MARCO (BioRad ED31), RORγt (654301), T-bet (644809). Antibodies were conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), PE-Cy7 or allophycocyanin (APC) and appropriate isotype controls were included in all experiments.
Subramanian K., Neill D.R., Malak H., Spelmink L., Khandaker S., Marchiori G.D., Dearing E., Kirby A., Yang M., Achour A., Nilvebrant J., Nygren P.Å., Plant L., Kadioglu A, & Henriques-Normark B. (2018). Pneumolysin binds to the Mannose-Receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival. Nature microbiology, 4(1), 62-70.
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