Mouse anti human tfr1

Unless otherwise stated, all chemicals including mouse monoclonal anti-β-actin were obtained from the Sigma Chemical Company, St. Louis, MO, USA. Mouse anti-human TfR1, Alexa Fluor 488 goat anti-rabbit IgG, TRIzol reagent, RPMI-1640 medium and fetal bovine serum were purchased from Invitrogen Life Technologies, Carlsbad, CA, USA; rabbit polyclonal anti-mouse Fpn1 from Novus Biologicals, Littleton, CO, USA; rabbit polyclonal anti-FTL (ferritin light chain) from Proteintech, Chicago, IL, USA; rabbit polyclonal anti-FTH (ferritin heavy chain) from Bioworld Technology Inc., Louis Park, MN, USA; rabbit anti-MAP2, rabbit anti-Aβ42, rabbit anti-HO1, rabbit anti-Gpx4, rabbit anti-ApoE, rabbit anti-IRP1 (iron regulatory protein 1) and rabbit anti-IRP2 (iron regulatory protein 2) from Abcam, Cambridge, MA, USA; and goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibody from LI-COR Biosciences, Lincoln, Nebraska, USA. AevertAid First Strand cDNA Synthesis Kit and BCA protein assay kits were bought from Thermo Scientific, Waltham, MA, USA; Faststart Universal SYBR Green Master and LightCycler96 from Roche, Nutley, NJ, USA; and protein RIPA lysis buffer from the Beyotime Institute of Biotechnology, Haimen, JS, China. All solutions were prepared fresh, prior to each assay.

Unless otherwise stated, all chemicals were obtained from the Sigma Chemical., St. Louis, MO, USA. Mouse anti-human TfR1, Alexa Fluor 488 goat anti-rabbit IgG, TRIzol reagent, RPMI-1640 medium and fetal bovine serum were purchased from Invitrogen Life Technologies, Carlsbad, CA, USA; rabbit polyclonal anti-mouse Fpn1 from Novus Biologicals, Littleton, CO, USA; rabbit polyclonal anti-FTL and anti-DMT1 from Proteintech, Chicago, IL, USA; rabbit anti-IRP1, rabbit anti-IRP2, rabbit anti-ApoE, F4/80 antibody and rabbit anti-Nrf2 antibody from Abcam, Cambridge, MA, USA; mouse anti-total p44/42-mitogen-activated protein kinases (MAPK) and rabbit anti-phosphorylated p44/42 MAPK (Thr202/Tyr204) polyclonal antibodies from Cell Signaling Technology Inc., Beverly, MA, USA; Goat anti-IRDye 800 CW secondary antibody from LI-COR Biosciences, Lincoln, Nebraska, USA. AevertAid First Strand cDNA Synthesis Kit and BCA protein assay kits were purchased from Thermo Scientific, Waltham, MA, USA; Faststart Universal SYBR Green Master and LightCycler96 from Roche, Nutley, NJ, USA; and protein RIPA lysis buffer from the Beyotime Institute of Biotechnology, Haimen, JS, China. All solutions were prepared fresh, prior to each assay.