Nf kb p65 transcription factor assay kit

Cardiac and serum TNF-α was detected using a mouse TNF-α enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Inc, Minneapolis, MN, USA) according to the manufacturer’s instructions. Cardiomyocyte nuclear NF-κB activity was measured using a NF-kB (p65) Transcription Factor Assay kit (Cayman Chemical, Ann Arbor, MI, USA) as previously described [24 (link)].

Human colorectal cancer cell lines (SW1116 and SW837) and normal human fibroblasts (CRL1554) were obtained from the American Type Culture Collection, ATCC (VA, USA). Leibovitz’s L-15 and EMEM (Eagle Minimum Essential Medium), trypsin, penicillin/streptomycin solution and fetal bovine serum (FBS) were obtained from Mediatech Inc. (Herndon, VA, USA). Primers, Taqman probes and all of the reagents for RT-PCR and real-time quantitative PCR (qPCR) were obtained from Applied Biosystems (Carlsbad, CA). The DNA-prep kit was obtained from Beckman & Coulter (Kendall, FL), and an Annexin V-FITC apoptosis detection kit was obtained from Hoffmann-La Roche Inc. (Nutley, NJ, USA). NFkB (p65) transcription factor assay kit was obtained from Cayman Chemical (Ann Arbor, MI, USA) and nuclear/cytosol fractionation kit was purchased from BioVision Inc. (Milipitas, CA, USA). Organic solvents of high-performance liquid chromatography (HPLC) grade were purchased from Fisher Scientific (Atlanta, GA, USA). Drugs, standard ferulic acid (FA) and other chemicals were obtained from Sigma-Aldrich Chemicals (St Louis, MO, USA).