Dionex chromeleon 7 chromatography data system software

Sample analysis was performed using a system comprised of a UHPLC Vanquish pump, Vanquish Autosampler, Vanquish UHPLC+ column compartment, and ISQ EC mass spectrometer (ThermoFisherSci.), utilizing ThermoFisherScientific Dionex Chromeleon 7 Chromatography Data System software, as described [17 (link), 18 (link)]. A C18 column (4.6 mm ID X 100 mm L, 5 μm, 120 Å; Acclaim 120; ThermoFisherSci.) was used with a 0.25 mL/min. flow rate and 0.01–1 μL injection volume; the autosampler needle was washed with 10% (v/v) methanol (10 s) after each sample injection. Mobile phases A and B consisted of 10 mM ammonium acetate and acetonitrile, respectively. The isocratic mobile phase flow was characterized by a uniform concentration in acetonitrile 50% between 0 to 7 or 14 min.

An assembled chromatography system [UHPLC Vanquish binary pump (prod. no. VFP10A01/121345), Vanquish auto-sampler (prod. no. VFA10A02/121345), and temperature-controlled Vanquish UHPLC+ column compartment (prod. no. VHC10A02/121345)] was coupled to an ISQ EC mass spectrometer (prod. no. ISQECLC/121345) (ThermoFisherScientific, Waltham, MA). Two chromatographic [Acclaim TM 120 C18 (4.6 mm ID × 100 mm L, 5μm, 120Å; prod. no. 059147, ThermoFisherSci.) and Shodex™ Asahipak™ NH2P-4 3E (3.0 mm ID × 250 mm L); no. M17T0005, Midland Scientific, Inc., La Vista, NE] columns were used to optimize mass spectrometric parameters for detection of D-(+)-Glucose-PMP. Column and autosampler temperatures were 35° C and 15° C, respectively. The auto-sampler needle was washed with 10% (v/v) methanol (10 sec). ThermoScientific™ Dionex™ Chromeleon™ 7 Chromatography Data System software (prod. no. 7200.0300/121345) was used for mass spectrometric analysis.