H+, K+-ATPase subunits detection was performed as described previously [15 (link)]. For signaling pathway analysis, PANC-1 cell lysate was boiled 3 min at 95 °C. Primary antibodies against gastric HKα1 (Abcam EPR12251, 1:1000), non-gastric HKα2 (C384-M79, 1:1000, kindly donated by J. J. H. H. M. De Pont and H. G. P. Swarts) [56 (link)], HKβ (Sigma A274, RRID:AB_258027, 1:1000), β-Actin (Santa Cruz Sc-47778, 1:1000, RRID: AB_626632), pSTAT3 (Cell Signaling #9145, 1:1000, RRID: AB_2491009), STAT3 (Cell Signaling #9139, 1:1000, RRID: AB_331757) and Cyclin D1 (Millipore #06-137, 1:500, RRID: AB_2070695) were used.
Sc 47778
Manufactured by Cell Signaling Technology
Sc-47778 is a laboratory product manufactured by Cell Signaling Technology. It is a reagent used for research purposes. No further details can be provided in an unbiased and factual manner without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Santa Cruz Biotechnology (1 mentions)
Ki16425, by Merck Group (1 mentions)
Reproneuro, by ReproCELL (1 mentions)
Horseradish peroxidase hrp conjugated anti mouse and anti rabbit secondary antibodies, by Cell Signaling Technology (1 mentions)
Gw9662, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 protocols using sc 47778
1
Immunoblotting Analysis of H+,K+-ATPase Subunits
2
Mature Human Neuron Differentiation from iPSCs
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To obtain mature human matured neurons in a culture system, ReproNeuro, a neuron progenitor derived from human iPSCs, was purchased from ReproCELL (Yokohama, Japan) and maintained in ReproNeuro maturation medium for 14 days according to the manufacturer's instructions. All assays were performed on cells between passages 3 and 12 and were repeated at least 3 times in duplicate or triplicate. LPA (18:1) was purchased from Cayman Chemical (Ann Arbor, MI); LPA was dissolved in phosphate-buffered saline containing 0.1% lipid-free bovine serum albumin (Sigma-Aldrich, MO) to generate a 10-mM stock solution. GW9662 and Ki16425 were purchased from Sigma-Aldrich (St. Louis, MO).
We used antibodies against PPARγ (rabbit monoclonal, sc-7196) and KLF9 (rabbit monoclonal, GTX129316), horseradish peroxidase (HRP)-conjugated anti-mouse and antirabbit secondary antibodies (Cell Signaling Technologies, Danvers, MA), and an antibody against β-actin (mouse monoclonal, sc-47778).
We used antibodies against PPARγ (rabbit monoclonal, sc-7196) and KLF9 (rabbit monoclonal, GTX129316), horseradish peroxidase (HRP)-conjugated anti-mouse and antirabbit secondary antibodies (Cell Signaling Technologies, Danvers, MA), and an antibody against β-actin (mouse monoclonal, sc-47778).
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