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3 protocols using curcumin

1

Curcumin Dendrosome Formulation Protocol

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Curcumin was purchased from Merck KGaA (Darmstadt, Germany) with purity of 95%. Materials for the preparation of dendrosome were oleoyl chloride and polyethylene glycol 400 that was purchased from Sigma-Aldrich (St Louis, MO, USA), and chloroform and triethyl amine were purchased from Merck. All the solvents used were high-performance liquid chromatography (HPLC) grade from Merck; 85% formic acid was provided by Carlo Erba Reagents (Cornaredo, Italy). Water was purified by a Milli-Q® Plus system from EMD Millipore (Billerica, MA, USA). Phosphate-buffered saline (PBS) solution was from Sigma-Aldrich. Curcumin reference standard (10 mg) was provided by Extrasynthese (Genay Cedex, France).
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2

Bioactive Phytochemical Formulations

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Lutein and Zeaxanthin (in the following denoted simply as lutein) in micellated formulation as well as the corresponding oil were obtained from Lipoid (Ludwigshafen, Germany), while lutein in powdered formulation was received from Divi’s Laboratories Europe AG (Basel, Switzerland). Analytical (all-E)-lutein and meso-zeaxanthin standards were purchased from Sigma Aldrich (St. Louis, LO, USA). Isoflavone in micellated formulation was obtained from Lipoid (Ludwigshafen, Germany), while the bulk isoflavone was retrieved from Frutarom (Londerzeel, Belgium). Analytical standards for the aglycones glycitein, daidzein and genistein as well as for the glycosylated forms (glycitin, daidzin, genistin) were purchased from Extrasynthese (Lyon, France). Curcuma extract complexed with γ-cyclodextrin was obtained from Wacker Chemie (Munich, Germany), while pure curcuma extract was purchased from Sigma Aldrich (St. Louis, LO, USA) and analytical curcuminoid standards (curcumin, demethoxycurcumin, bisdemethoxycurcumin) were purchased from Extrasynthese (Lyon, France). Black pepper extract was provided by Sabinsa Europe GmbH (Langen, Germany) and ginger extract was obtained from Vivatis Pharma (Hamburg, Germany).
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3

Curcumin Cell Culture Assay

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Curcumin was purchased from Extrasynthese (France). Reagents for cell culture (RPMI 1640, penicillin/streptomycin, Trypsin/EDTA, Foetal Bovine Serum) were purchased from PAN biotech (Germany). Gelatin, paraformaldehyde (PFA), FITC-dextran, dimethylsulfoxide (DMSO), antiprotease cocktail, anti-phosphatase cocktail, Nonidet P40 and DAPI were obtained from Sigma-Aldrich (France). Recombinant-human-TNF- and MCP-1 were obtained from R&D systems and Miltenyi Biotec SAS (Paris, France) respectively.
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