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2 protocols using 2 4 dimethoxybenzylamine

1

Photocurable Hydrogel Synthesis

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Poly(ethylene glycol) diacrylate (PEGDA, MW=700 Da), butanediol diacrylate (BDDA), decylamine, isobutylamine, benzylamine, 2,4-Dimethoxybenzylamine, diphenyl(2,4,6-trimethylbenzoyl) phosphine oxide (TPO), dimethyl sulfoxide (DMSO) and tetrahydrofuran (THF) were purchased from Sigma-Aldrich. Benzhydrazide and phenylacetic hydrazide were purchased from Alfa Aesar. Dulbecco’s phosphate buffered saline (PBS) was obtained from Corning.
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2

Synthesis of Fluorescent Conjugates

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Bromoethanol, p-nitrophenyl
chloroformate, 3-toluenesulfonyl chloride, triethylamine, 2,4-quinolinediol,
phenylphosphonyl dichloride, p-xylylendiamine, valeroyl
chloride, calcium oxide, di-tert-butyl decarbonate,
deuterated solvents, ethyl acetate, dimethyl sulfoxide (DMSO), sodium
hydroxide (NaOH), hydrochloric acid (HCl), 2,4-dimethoxybenzyl amine,
trifluoroacetic acid (TFA), platinum on carbon (10% Pt/C), benzylamine,
benzyl alcohol, dibenzylamine, 4-dimethylaminopyridine (DMAP), 2,2′-azobis(2-methylpropionitrile)
(AIBN), n-butylamine, N,N-dimethylacrylamide (DMA), sodium bicarbonate (NaHCO3), cholesterol, acryloxyethyl thiocarbamoyl rhodamine B, 2-mercaptoethanol,
2,2′-dipyridyl disulfide, and N,N′-dicyclohexylcarbodiimide (DCC) were obtained from Sigma-Aldrich.
Potassium sulfite, sulfur, tris(2-carboxyethyl)phosphin (TCEP), and
sodium sulfate were purchased from Acros Organics, Alfa Aesar, TCI
Chemicals, and Carl Roth GmbH+ Co. KG, respectively. N,N-Dimethylformamide (DMF), chloroform, dichloromethane
(DCM), tetrahydrofuran (THF), succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate
(SMCC), and Gibco Dulbecco’s phosphate-buffered saline (DPBS)
were obtained from Thermo Fisher Scientific Inc., and methanol (MeOH),
acetone, and ethanol were obtained from Honeywell International.
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